Military Medical Research,
Год журнала:
2023,
Номер
10(1)
Опубликована: Март 10, 2023
Abstract
The
rapid
development
of
genome
editing
technology
has
brought
major
breakthroughs
in
the
fields
life
science
and
medicine.
In
recent
years,
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)-based
toolbox
been
greatly
expanded,
not
only
with
emerging
CRISPR-associated
protein
(Cas)
nucleases,
but
also
novel
applications
through
combination
diverse
effectors.
Recently,
transposon-associated
programmable
RNA-guided
systems
have
uncovered,
adding
myriads
potential
new
tools
to
toolbox.
CRISPR-based
revolutionized
cardiovascular
research.
Here
we
first
summarize
advances
involving
newly
identified
Cas
orthologs,
engineered
variants
systems,
then
discuss
CRISPR-Cas
precise
editing,
such
as
base
prime
editing.
We
highlight
progress
research
using
technologies,
including
generation
genetically
modified
vitro
animal
models
diseases
(CVD)
well
treating
different
types
CVD.
Finally,
current
limitations
future
prospects
technologies
are
discussed.
Analytical Chemistry,
Год журнала:
2022,
Номер
94(23), С. 8277 - 8284
Опубликована: Май 30, 2022
CRISPR/Cas12,
a
highly
efficient
and
specific
nucleic
acid
recognition
system,
has
been
broadly
employed
to
detect
amplified
DNA
products.
However,
most
reported
methods
adopt
two-step
detection
mode
that
needs
liquid
transfer
step,
thus
complicating
the
procedure
posing
risk
of
aerosol
contamination.
A
one-pot
method
can
obviate
these
problems,
but
it
suffers
from
poor
efficiency
due
loss
amplification
templates
elicited
by
CRISPR/Cas12
cleavage.
In
this
study,
we
discovered
glycerol
additive
dramatically
promoted
recombinase
polymerase
(RPA)-CRISPR/Cas12a
method.
Compared
with
glycerol-free
version,
its
sensitivity
was
nearly
100-fold
higher
close
canonical
Further
investigation
displayed
enhanced
attributed
phase
separation
RPA
CRISPR/Cas12a
system
during
initial
reaction
caused
viscosity.
This
triumphantly
harnessed
for
African
swine
fever
virus
(ASFV)
SARS-CoV-2,
achieving
naked-eye
readout
through
smartphone-equipped
device.
The
currently
developed
glycerol-enhanced
RPA-CRISPR/Cas12a
be
an
advantageous
point-of-care
platform
on
account
simplicity,
high
sensitivity,
universality.
Proceedings of the National Academy of Sciences,
Год журнала:
2022,
Номер
119(30)
Опубликована: Июль 18, 2022
The
rediscovery
of
Mendel's
work
showing
that
the
heredity
phenotypes
is
controlled
by
discrete
genes
was
followed
reconciliation
Mendelian
genetics
with
evolution
natural
selection
in
middle
last
century
Modern
Synthesis.
In
past
two
decades,
dramatic
advances
genomic
methods
have
facilitated
identification
loci,
genes,
and
even
individual
mutations
underlie
phenotypic
variants
are
putative
targets
selection.
Moreover,
these
also
changed
how
we
can
study
adaptation
flipping
problem
around,
allowing
us
to
first
examine
what
loci
show
evidence
having
been
under
selection,
then
connecting
genetic
variation.
As
a
result,
now
an
expanding
list
actual
changes
potentially
adaptive
Here,
synthesize
considering
effects
context
cellular
environments,
genomes,
organisms,
populations
has
provided
new
insights
architecture
adaptation.
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(23)
Опубликована: Апрель 5, 2023
Abstract
The
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
system
is
a
promising
platform
for
nucleic
acid
detection.
Regulating
the
CRISPR
reaction
would
be
extremely
useful
to
improve
detection
efficiency
and
speed
of
diagnostic
applications.
Here,
we
have
developed
light‐start
CRISPR‐Cas12a
by
employing
caged
RNA
(crRNA).
When
combined
with
recombinase
polymerase
amplification,
robust
photocontrolled
one‐pot
assay
achieved.
simpler
50‐fold
more
sensitive
than
conventional
assay.
This
improved
also
facilitates
development
faster
method.
clinical
samples
demonstrated
that
10–20
min
sufficient
effective
detection,
which
much
current
gold‐standard
technique
PCR.
We
expect
this
advance
in
diagnostics
promote
its
widespread
applications
biomedicine,
agriculture,
food
safety.
Nature Biotechnology,
Год журнала:
2023,
Номер
41(10), С. 1446 - 1456
Опубликована: Фев. 16, 2023
Most
short
sequences
can
be
precisely
written
into
a
selected
genomic
target
using
prime
editing;
however,
it
remains
unclear
what
factors
govern
insertion.
We
design
library
of
3,604
various
lengths
and
measure
the
frequency
their
insertion
four
sites
in
three
human
cell
lines,
different
editor
systems
varying
DNA
repair
contexts.
find
that
length,
nucleotide
composition
secondary
structure
sequence
all
affect
rates.
also
discover
3'
flap
nucleases
TREX1
TREX2
suppress
longer
sequences.
Combining
features
machine
learning
model,
we
predict
relative
insertions
site
with
R
=
0.70.
Finally,
demonstrate
how
our
accurate
prediction
user-friendly
software
help
choose
codon
variants
common
fusion
tags
insert
at
high
efficiency,
provide
catalog
empirically
determined
rates
for
over
hundred
useful
