Trends in biotechnology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Plant Cell Reports, Год журнала: 2025, Номер 44(1)
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
Plant Biotechnology Journal, Год журнала: 2025, Номер unknown
Опубликована: Фев. 12, 2025
Summary The integration of haploid induction and genome editing, termed HI‐Edit/IMGE, is a promising tool for generating targeted mutations crop breeding. However, the technical components stacking suitable maize seed industry have yet to be fully characterised tested. Here, we developed assessed three HI‐Edit/IMGE lines: Edit Wx , Sh & using inducer CHOI3 lines engineered CRISPR‐Cas9 system targeting Waxy1 ( Wx1 ) Shrunken2 Sh2 genes. We meticulously systems, focusing on copy numbers mutant alleles mtl dmp which facilitate induction. Using B73 six other parental major commercial varieties as recipients, demonstrated maternal efficiencies ranging from 8.55% 20.89% mutation rates between 0.38% 1.46%. Comprehensive assessment verified identification, target gene editing accuracy, background integrity, related agronomic traits. Notably, successfully combined distinct systems induce multiple desired mutations, highlighting potential in accelerating edited traits into varieties. Our findings underscore importance meticulous Cas9 number characterisation highlight challenges somatic chimerism. also validated performance single‐cross haploids derived process. results confirm industrial applicability through pollination provide critical insights further optimising this technology.
Язык: Английский
Процитировано
1
Nature Reviews Molecular Cell Biology, Год журнала: 2025, Номер unknown
Опубликована: Март 7, 2025
Язык: Английский
Процитировано
1
New Phytologist, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Genome editing for the development of improved varieties is supported by possibility segregating out editor T-DNA cassette after genome in many crop species. Removal prevents potential continuous activity transformed plant and furthermore facilitates regulatory approval. While transgene outcrossing exogenous sequences possible crops, this not case vegetatively propagated sterile such as Cavendish bananas. Therefore, gene techniques leading to transgene-free edited plants are essential untap those crops. Here, we present a method banana (Musa spp.) through co-editing strategy. A novel Agrobacterium tumefaciens-mediated approach combining embryogenesis chlorsulfuron selection was established validated whole sequencing. Editing acetolactate synthase (MaALS) genes using base allows effective plants. Moreover, plantlets were regenerated with mutations at two target sites, indicating that strategy can be used multiple genomic sites. The presented efficient represents first report
Язык: Английский
Процитировано
1
Sustainability, Год журнала: 2024, Номер 16(14), С. 6026 - 6026
Опубликована: Июль 15, 2024
As valuable sources of plant-based protein, leguminous vegetables (grain legumes) are essential for global food security and contribute to body growth development in humans as well animals. Climate change is a major challenge agriculture that creates problems the plants. However, legume productivity threatened by climate factors, including rising temperatures, shifting precipitation patterns, increased atmospheric carbon dioxide levels, intensified extreme events, altered pest/pathogen activity. This review synthesizes approximately 136 studies assess effects on crops. Under all emissions trajectories, mean temperatures projected rise beyond optimal growing thresholds 2050, carrying yield reductions between 10 49% beans, soybeans, cowpeas, lentils without adaptation measures. The elevated may transiently enhance yields up 18%, but benefits dramatically decline above 550 ppm cannot offset other impacts. Altered rainfall along with recurrent drought heat waves also expected decrease crop yields, seed quality, soil nitrogen levels worldwide. Furthermore, proliferation pests fungal diseases poses significant risks, amplified shifts 84% reviewed studies. These multifaceted impacts threaten gains sustainably meeting protein demand. Realizing resilience will require accelerated heat/drought-tolerant varieties, enhanced climate-informed agronomic practices, strong policy interventions, social safety nets explicitly supporting producers, addition policies/steps governments taking address challenges crisis. highlights adaptations mechanisms required crops thrive fulfill their roles nutrition. It explores how these can be improved better withstand environmental stresses, nutritional profiles, increase yields. Additionally, discusses importance legumes sustainable security, emphasizing potential future feeding population. By focusing critical aspects, aims underscore ensuring healthy supply.
Язык: Английский
Процитировано
7
Journal of Integrative Plant Biology, Год журнала: 2024, Номер unknown
Опубликована: Окт. 28, 2024
ABSTRACT The past decade has witnessed rapid developments in gene discovery, biological big data (BBD), artificial intelligence (AI)‐aided technologies, and molecular breeding. These advancements are expected to accelerate crop breeding under the pressure of increasing demands for food. Here, we first summarize current methods discuss need new ways support efforts. Then, review how combine BBD AI technologies genetic dissection, exploring functional genes, predicting regulatory elements domains, phenotypic prediction. Finally, propose concept intelligent precision design (IPDB) driven by technology offer ideas about implement IPDB. We hope that IPDB will enhance predictability, efficiency, cost compared with technologies. As an example IPDB, explore possibilities offered CropGPT, which combines techniques, bioinformatics, art from breeders, presents open, shareable, cooperative system. provides integrated services communication platforms biologists, bioinformatics experts, germplasm resource specialists, dealers, farmers, should be well suited future
Язык: Английский
Процитировано
5
Plant Cell & Environment, Год журнала: 2024, Номер unknown
Опубликована: Окт. 28, 2024
ABSTRACT Heterotrophic microbes rely on host‐derived carbon sources for their growth and survival. Depriving pathogens of plant is therefore a promising strategy protecting plants from disease reducing yield losses. Importantly, this starvation‐mediated resistance expected to be more broad‐spectrum durable than race‐specific R ‐gene‐mediated resistance. Although sugars are well characterized as major bacteria, emerging evidence suggests that plant‐derived lipids likely an essential source some fungal microbes, particularly biotrophs. Here, we comprehensively discuss the dual roles (mainly lipids) transport processes in immune signalling microbial nutrition. We summarize recent findings revealing crucial susceptibility factors at all stages pathogen infection. In particular, potential pathways by which other delivered biotrophs, including protein‐mediated transport, vesicle trafficking autophagy. Finally, highlight knowledge gaps offer suggestions clarifying mechanisms underlie nutrient uptake providing guidance future research application starvation–mediated
Язык: Английский
Процитировано
4
Trends in Plant Science, Год журнала: 2025, Номер 30(3), С. 232 - 234
Опубликована: Янв. 2, 2025
Язык: Английский
Процитировано
0
Current Plant Biology, Год журнала: 2025, Номер unknown, С. 100433 - 100433
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Industrial Crops and Products, Год журнала: 2025, Номер 225, С. 120446 - 120446
Опубликована: Янв. 8, 2025
Язык: Английский
Процитировано
0