Journal of Nanoparticle Research, Год журнала: 2024, Номер 26(8)
Опубликована: Авг. 1, 2024
Язык: Английский
Neuroscience, Год журнала: 2024, Номер 565, С. 63 - 79
Опубликована: Ноя. 26, 2024
Язык: Английский
Процитировано
6
Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 10
Опубликована: Апрель 20, 2022
Considerable progress has been made in our knowledge of the morphological and functional varieties anchoring junctions. Cell-cell adhesion contacts consist discrete junctional structures responsible for mechanical coupling cytoskeletons allow transmission signals across cell collective. The three main complexes are adherens junctions, tight desmosomes. Microscopy played a fundamental role understanding these on different levels both physiological pathological conditions. In this review, we discuss light electron microscopy techniques used to unravel structure composition cell-cell epithelial endothelial cells. It functions as guide pick appropriate imaging technique(s) interest. We also point out latest that have emerged. At end, problems investigators encounter during their research using microscopic techniques.
Язык: Английский
Процитировано
22
Cell Reports, Год журнала: 2024, Номер 43(5), С. 114170 - 114170
Опубликована: Май 1, 2024
During cell fate transitions, cells remodel their transcriptome, chromatin, and epigenome; however, it has been difficult to determine the temporal dynamics cause-effect relationship between these changes at single-cell level. Here, we employ heterokaryon-mediated reprogramming system as a model dissect key events during early stages of pluripotency conversion using super-resolution imaging. We reveal that, following heterokaryon formation, somatic nucleus undergoes global chromatin decompaction removal repressive histone modifications H3K9me3 H3K27me3 without acquisition active H3K4me3 H3K9ac. The gene OCT4 (POU5F1) shows nascent mature RNA transcription within first 24 h after fusion requiring an initial open configuration its locus. NANOG, conversely, significant only 48 but, strikingly, exhibits genomic reopening on. These findings suggest that compaction activation cellular is context dependent.
Язык: Английский
Процитировано
5
Trends in Food Science & Technology, Год журнала: 2024, Номер 152, С. 104674 - 104674
Опубликована: Авг. 18, 2024
Язык: Английский
Процитировано
5
Science Advances, Год журнала: 2024, Номер 10(39)
Опубликована: Сен. 25, 2024
Over the past decades, single-molecule and super-resolution microscopy have advanced represent essential tools for life science research. There is, however, a growing gap between state of art what is accessible to biologists, biochemists, medical researchers, or labs with financial constraints. To bridge this gap, we introduce Brick-MIC, versatile affordable open-source 3D-printed microspectroscopy imaging platform. Brick-MIC enables integration various fluorescence techniques resolution within single platform exchange different modalities minutes. We here present variants that facilitate detection, correlation spectroscopy, time-correlated single-photon counting (STORM PAINT). Detailed descriptions hardware software components, as well data analysis routines, are provided, allow non-optics specialists operate their own minimal effort investments. foresee our affordable, flexible, will be valuable tool many laboratories worldwide.
Язык: Английский
Процитировано
5
Frontiers of Optoelectronics, Год журнала: 2023, Номер 16(1)
Опубликована: Ноя. 10, 2023
Abstract The development of super-resolution technology has made it possible to investigate the ultrastructure intracellular organelles by fluorescence microscopy, which greatly facilitated life sciences and biomedicine. To realize imaging living cells, both advanced systems excellent fluorescent probes are required. Traditional have good availability, but that is not case for live-cell imaging. In this review, we first introduce principles various technologies their probe requirements, then summarize existing designs delivery strategies imaging, finally provide a brief conclusion overview future. Graphical
Язык: Английский
Процитировано
10
Optics Letters, Год журнала: 2023, Номер 49(1), С. 77 - 77
Опубликована: Дек. 4, 2023
Structured illumination microscopy (SIM) achieves super-resolution imaging using a series of phase-shifted sinusoidal patterns to down-modulate high spatial-frequency information samples. Digital micromirror devices (DMDs) have been increasingly used generate SIM due their speed and moderate cost. However, DMD array's blazed grating structure causes strong angular dispersion for different wavelengths light, thus severely hampering its application in multicolor imaging. We developed multi-color DMD-SIM setup that employs diffraction compensate the DMD's demonstrate both fluorescent beads live cells samples with four color channels. This simple but effective approach can be readily scaled more channels, thereby greatly expanding study complex multi-component structures dynamics soft matter systems.
Язык: Английский
Процитировано
10
Current Opinion in Chemical Biology, Год журнала: 2024, Номер 80, С. 102445 - 102445
Опубликована: Март 14, 2024
Super-resolution microscopy (SRM) has transformed our understanding of proteins' subcellular organization and revealed cellular details down to nanometers, far beyond conventional microscopy. While localization precision is independent the number fluorophores attached a biomolecule, labeling density decisive factor for resolving complex biological structures. The average distance between adjacent should be less than half desired spatial resolution optimal clarity. this was not major limitation in recent decades, success modern approaching molecular single-digit nanometer range will depend heavily on advancements fluorescence labeling. This review highlights advances challenges strategies SRM, focusing site-specific technologies. These are crucial improving SRM expanding interactions.
Язык: Английский
Процитировано
4
The Journal of Cell Biology, Год журнала: 2024, Номер 224(1)
Опубликована: Ноя. 1, 2024
Late endosomes/lysosomes (LELs) are crucial for numerous physiological processes and their dysfunction is linked to many diseases. Proteomic analyses have identified hundreds of LEL proteins; however, whether these proteins uniformly present on each LEL, or if there cell-type-dependent subpopulations with unique protein compositions unclear. We employed quantitative, multiplexed DNA-PAINT super-resolution imaging examine the distribution seven key (LAMP1, LAMP2, CD63, Cathepsin D, TMEM192, NPC1, LAMTOR4). While LAMP1, D were abundant across LELs, marking a common population, most analyzed associated specific subpopulations. Our approach up eight different based membrane composition. Additionally, our analysis spatial relationships between mitochondria revealed cell-type-specific tendency NPC1-positive LELs be closely positioned mitochondria. will broadly applicable determining organelle heterogeneity single resolution in biological contexts.
Язык: Английский
Процитировано
4
Biophysics Reviews, Год журнала: 2023, Номер 4(2)
Опубликована: Май 22, 2023
Neutron scattering methods are powerful tools for the study of structure and dynamics lipid bilayers in length scales from sub Å to tens hundreds nm time ps μs. These techniques also nondestructive and, perhaps most importantly, require no additives label samples. Because neutron intensities very different hydrogen- deuterium-containing molecules, one can replace hydrogen atoms a molecule with deuterium prepare on demand contrast without significantly altering physical properties Moreover, recent advances techniques, membrane theories, analysis tools, sample preparation technologies allow researchers various aspects bilayer dynamics. In this review, we focus individual lipids collective as well hydration water.
Язык: Английский
Процитировано
9