Journal of Proteome Research,
Год журнала:
2023,
Номер
22(12), С. 3773 - 3779
Опубликована: Ноя. 1, 2023
Accurate
measurements
of
the
molecular
composition
single
cells
will
be
necessary
for
understanding
relationship
between
gene
expression
and
function
in
diverse
cell
types.
One
most
important
phenotypes
that
differs
is
their
size,
which
was
recently
shown
to
an
determinant
proteome
populations
similarly
sized
cells.
We,
therefore,
sought
test
if
effects
size
on
protein
concentrations
were
also
evident
single-cell
proteomics
data.
Using
relative
a
set
reference
proteins
estimate
cell's
DNA-to-cell
volume
ratio,
we
found
differences
explain
significant
amount
cell-to-cell
variance
two
published
data
sets.
Nature Structural & Molecular Biology,
Год журнала:
2023,
Номер
30(10), С. 1549 - 1560
Опубликована: Сен. 7, 2023
To
maintain
stable
DNA
concentrations,
proliferating
cells
need
to
coordinate
replication
with
cell
growth.
For
nuclear
DNA,
eukaryotic
achieve
this
by
coupling
cell-cycle
progression,
ensuring
that
is
doubled
exactly
once
per
cycle.
By
contrast,
mitochondrial
typically
not
strictly
coupled
the
cycle,
leaving
open
question
of
how
correct
amount
during
Here,
we
show
in
budding
yeast,
copy
number
increases
volume,
both
asynchronously
cycling
populations
and
G1
arrest.
Our
findings
suggest
cell-volume-dependent
maintenance
achieved
through
nuclear-encoded
limiting
factors,
including
polymerase
Mip1
packaging
factor
Abf2,
whose
proportion
volume.
directly
linking
protein
synthesis
thus
growth,
constant
concentrations
can
be
robustly
maintained
without
a
for
cell-cycle-dependent
regulation.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Фев. 17, 2025
Dynamic
changes
in
cell
size
are
associated
with
development
and
pathological
conditions,
including
aging.
Although
enlargement
is
a
prominent
morphological
feature
of
cellular
senescence,
its
functional
implications
unknown;
moreover,
how
senescent
cells
maintain
their
state
less
understood.
Here
we
show
that
an
extensive
remodeling
actin
cytoskeleton
necessary
for
establishing
senescence-associated
pro-inflammatory
secretory
phenotype
(SASP).
This
attributed
to
balancing
act
between
the
SASP
regulator
GATA4
mechanosensor
YAP
on
expression
Rho
family
GTPase
RHOU.
Genetic
or
pharmacological
interventions
reduce
attenuate
minimal
effect
senescence
growth
arrest.
Mechanistically,
couples
nuclear
localization
NF-κB
via
Linker
Nucleoskeleton
Cytoskeleton
(LINC)
complex.
RhoU
protein
accumulates
mouse
adipose
tissue
under
senescence-inducing
conditions.
Furthermore,
RHOU
correlates
during
human
Thus,
our
study
highlights
unexpected
instructive
role
modulating
reveals
mechanical
branch
regulatory
network.
Senescent
accumulate
aging
exhibit
enlargement,
function
which
has
been
unclear
decades.
Here,
authors
identify
antagonistic
genetic
circuit
hypertrophy
reveal
SASP.
Annual Review of Genetics,
Год журнала:
2022,
Номер
56(1), С. 165 - 185
Опубликована: Авг. 17, 2022
Though
cell
size
varies
between
different
cells
and
across
species,
the
nuclear-to-cytoplasmic
(N/C)
ratio
is
largely
maintained
species
within
types.
A
maintains
a
relatively
constant
N/C
by
coupling
DNA
content,
nuclear
size,
size.
We
explore
how
couple
division
growth
to
content.
In
some
cases,
use
as
molecular
yardstick
control
availability
of
cycle
regulators.
other
sets
limit
for
biosynthetic
capacity.
Developmentally
programmed
variations
in
given
type
suggest
that
specific
required
respond
physiological
demands.
Recent
observations
connecting
decreased
ratios
with
cellular
senescence
indicate
maintaining
proper
essential
functioning.
Together,
these
findings
causative,
not
simply
correlative,
role
regulating
progression.
Abstract
How
the
production
of
biomass
is
controlled
as
cells
increase
in
size
and
proceed
through
cell
cycle
events
important
for
understanding
regulation
global
cellular
growth.
This
has
been
studied
decades
but
not
yielded
consistent
results,
probably
due
to
perturbations
induced
by
synchronisation
methods
used
most
previous
studies.
To
avoid
this
problem,
we
have
developed
a
system
analyse
unperturbed
exponentially
growing
populations
fission
yeast
cells.
We
generated
thousands
fixed
single‐cell
measurements
size,
stage
levels
translation
transcription.
show
that
scales
with
additionally,
increases
at
late
S‐phase/early
G2
early
mitosis
decreases
later
mitosis,
suggesting
controls
are
also
operative
over
translation.
Transcription
both
amount
DNA,
level
transcription
may
be
result
dynamic
equilibrium
between
number
RNA
polymerases
associating
disassociating
from
DNA.
Current Biology,
Год журнала:
2023,
Номер
33(9), С. 1744 - 1752.e7
Опубликована: Апрель 19, 2023
Although
polyploidization
is
frequent
in
development,
cancer,
and
evolution,
impacts
on
animal
metabolism
are
poorly
understood.
In
Xenopus
frogs,
the
number
of
genome
copies
(ploidy)
varies
across
species
can
be
manipulated
within
a
species.
Here,
we
show
that
triploid
tadpoles
contain
fewer,
larger
cells
than
diploids
consume
oxygen
at
lower
rate.
Drug
treatments
revealed
major
processes
accounting
for
tadpole
energy
expenditure
include
cell
proliferation,
biosynthesis,
maintenance
plasma
membrane
potential.
While
inhibiting
proliferation
did
not
abolish
consumption
difference
between
triploids,
altered
cellular
biosynthesis
or
electrical
potential
did.
Combining
these
results
with
simple
mathematical
framework,
propose
decrease
total
surface
area
lowered
production
activity
components
including
Na
