Cell Size Contributes to Single-Cell Proteome Variation DOI
Michael C. Lanz, Lucas Fuentes Valenzuela, Joshua E. Elias

et al.

Journal of Proteome Research, Journal Year: 2023, Volume and Issue: 22(12), P. 3773 - 3779

Published: Nov. 1, 2023

Accurate measurements of the molecular composition single cells will be necessary for understanding relationship between gene expression and function in diverse cell types. One most important phenotypes that differs is their size, which was recently shown to an determinant proteome populations similarly sized cells. We, therefore, sought test if effects size on protein concentrations were also evident single-cell proteomics data. Using relative a set reference proteins estimate cell's DNA-to-cell volume ratio, we found differences explain significant amount cell-to-cell variance two published data sets.

Language: Английский

Regulation with cell size ensures mitochondrial DNA homeostasis during cell growth DOI Creative Commons

Anika Seel,

Francesco Padovani,

Moritz Mayer

et al.

Nature Structural & Molecular Biology, Journal Year: 2023, Volume and Issue: 30(10), P. 1549 - 1560

Published: Sept. 7, 2023

To maintain stable DNA concentrations, proliferating cells need to coordinate replication with cell growth. For nuclear DNA, eukaryotic achieve this by coupling cell-cycle progression, ensuring that is doubled exactly once per cycle. By contrast, mitochondrial typically not strictly coupled the cycle, leaving open question of how correct amount during Here, we show in budding yeast, copy number increases volume, both asynchronously cycling populations and G1 arrest. Our findings suggest cell-volume-dependent maintenance achieved through nuclear-encoded limiting factors, including polymerase Mip1 packaging factor Abf2, whose proportion volume. directly linking protein synthesis thus growth, constant concentrations can be robustly maintained without a for cell-cycle-dependent regulation.

Language: Английский

Citations

27
The bidirectional relationship between metabolism and cell cycle control DOI Creative Commons

Frances F. Diehl,

Kiera M. Sapp, Matthew G. Vander Heiden

et al.

Trends in Cell Biology, Journal Year: 2023, Volume and Issue: 34(2), P. 136 - 149

Published: June 27, 2023

Language: Английский

Citations

26
Metabolic remodeling in cancer and senescence and its therapeutic implications DOI
Yeonju Kim, Yeji Jang, Mi-Sung Kim

et al.

Trends in Endocrinology and Metabolism, Journal Year: 2024, Volume and Issue: 35(8), P. 732 - 744

Published: March 6, 2024

Language: Английский

Citations

12
Deep proteomic analysis of microglia reveals fundamental biological differences between model systems DOI Creative Commons
Amy Lloyd, Anna Martínez‐Muriana, Emma Davis

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(11), P. 114908 - 114908

Published: Oct. 25, 2024

Language: Английский

Citations

11
Cell enlargement modulated by GATA4 and YAP instructs the senescence-associated secretory phenotype DOI Creative Commons

J. P. W. Joung,

Y.‐A. Heo,

Yeonju Kim

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 17, 2025

Dynamic changes in cell size are associated with development and pathological conditions, including aging. Although enlargement is a prominent morphological feature of cellular senescence, its functional implications unknown; moreover, how senescent cells maintain their state less understood. Here we show that an extensive remodeling actin cytoskeleton necessary for establishing senescence-associated pro-inflammatory secretory phenotype (SASP). This attributed to balancing act between the SASP regulator GATA4 mechanosensor YAP on expression Rho family GTPase RHOU. Genetic or pharmacological interventions reduce attenuate minimal effect senescence growth arrest. Mechanistically, couples nuclear localization NF-κB via Linker Nucleoskeleton Cytoskeleton (LINC) complex. RhoU protein accumulates mouse adipose tissue under senescence-inducing conditions. Furthermore, RHOU correlates during human Thus, our study highlights unexpected instructive role modulating reveals mechanical branch regulatory network. Senescent accumulate aging exhibit enlargement, function which has been unclear decades. Here, authors identify antagonistic genetic circuit hypertrophy reveal SASP.

Language: Английский

Citations

1
Trends in Mass Spectrometry-Based Single-Cell Proteomics DOI

Ximena Sanchez-Avila,

Raphaela Menezes de Oliveira, Siqi Huang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

InfoMetrics Analytical ChemistryASAPArticle CiteCitationCitation and abstractCitation referencesMore citation options ShareShare onFacebookXWeChatLinkedInRedditEmailBlueskyJump toExpandCollapse ReviewMarch 16, 2025Trends in Mass Spectrometry-Based Single-Cell ProteomicsClick to copy article linkArticle link copied!Ximena Sanchez-AvilaXimena Sanchez-AvilaDepartment of Chemistry Biochemistry, Brigham Young University, Provo, Utah 84602, United StatesMore by Ximena Sanchez-AvilaView BiographyRaphaela M. de OliveiraRaphaela OliveiraDepartment Raphaela OliveiraView BiographySiqi HuangSiqi HuangDepartment Siqi HuangView BiographyChao WangChao WangDepartment Chao WangView Biographyhttps://orcid.org/0009-0008-6197-2985Ryan T. Kelly*Ryan KellyDepartment States*Email: [email protected]More Ryan KellyView Biographyhttps://orcid.org/0000-0002-3339-4443Other Access OptionsAnalytical ChemistryCite this: Anal. Chem. 2025, XXXX, XXX, XXX-XXXClick citationCitation copied!https://pubs.acs.org/doi/10.1021/acs.analchem.5c00661https://doi.org/10.1021/acs.analchem.5c00661Published March 2025 Publication History Received 28 January 2025Accepted February 2025Revised 23 2025Published online 16 2025review-article© American Chemical SocietyRequest reuse permissionsACS Publications© SocietySubjectswhat are subjects Article automatically applied from the ACS Subject Taxonomy describe scientific concepts themes article. Cells Isolation spectrometry Peptides proteins Sample preparation Note: In lieu an abstract, this is article's first page. Read To access article, please review available below. Get instant Purchase for 48 hours. Check out below using your ID or as a guest. Restore my guest Recommended through Your Institution You may have institution. institution does not content. Add change let them know you'd like include access. Through Recommend Name Loading Institutional Login Options... Change Explore subscriptions institutions Log with if you previously purchased it member benefits. hours $48.00 cart Checkout Cited By Click section linkSection copied!This has yet been cited other publications.Download PDF e-AlertsGet e-AlertsAnalytical copied!https://doi.org/10.1021/acs.analchem.5c00661Published 2025© permissionsArticle Views6Altmetric-Citations-Learn about these metrics closeArticle Views COUNTER-compliant sum full text downloads since November 2008 (both HTML) across all individuals. These regularly updated reflect usage leading up last few days.Citations number articles citing calculated Crossref daily. Find more information counts.The Altmetric Attention Score quantitative measure attention that research received online. Clicking on donut icon will load page at altmetric.com additional details score social media presence given how calculated.Recommended Articles

Language: Английский

Citations

1
The Nuclear-to-Cytoplasmic Ratio: Coupling DNA Content to Cell Size, Cell Cycle, and Biosynthetic Capacity DOI Open Access

Shruthi Balachandra,

Sharanya Sarkar, Amanda A. Amodeo

et al.

Annual Review of Genetics, Journal Year: 2022, Volume and Issue: 56(1), P. 165 - 185

Published: Aug. 17, 2022

Though cell size varies between different cells and across species, the nuclear-to-cytoplasmic (N/C) ratio is largely maintained species within types. A maintains a relatively constant N/C by coupling DNA content, nuclear size, size. We explore how couple division growth to content. In some cases, use as molecular yardstick control availability of cycle regulators. other sets limit for biosynthetic capacity. Developmentally programmed variations in given type suggest that specific required respond physiological demands. Recent observations connecting decreased ratios with cellular senescence indicate maintaining proper essential functioning. Together, these findings causative, not simply correlative, role regulating progression.

Language: Английский

Citations

38
The cell cycle and cell size influence the rates of global cellular translation and transcription in fission yeast DOI Creative Commons
Clovis Basier, Paul Nurse

The EMBO Journal, Journal Year: 2023, Volume and Issue: 42(9)

Published: March 23, 2023

Abstract How the production of biomass is controlled as cells increase in size and proceed through cell cycle events important for understanding regulation global cellular growth. This has been studied decades but not yielded consistent results, probably due to perturbations induced by synchronisation methods used most previous studies. To avoid this problem, we have developed a system analyse unperturbed exponentially growing populations fission yeast cells. We generated thousands fixed single‐cell measurements size, stage levels translation transcription. show that scales with additionally, increases at late S‐phase/early G2 early mitosis decreases later mitosis, suggesting controls are also operative over translation. Transcription both amount DNA, level transcription may be result dynamic equilibrium between number RNA polymerases associating disassociating from DNA.

Language: Английский

Citations

18
Polyploidy in Xenopus lowers metabolic rate by decreasing total cell surface area DOI Creative Commons
Clotilde Cadart, Julianne Bartz,

Gillian Oaks

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(9), P. 1744 - 1752.e7

Published: April 19, 2023

Although polyploidization is frequent in development, cancer, and evolution, impacts on animal metabolism are poorly understood. In Xenopus frogs, the number of genome copies (ploidy) varies across species can be manipulated within a species. Here, we show that triploid tadpoles contain fewer, larger cells than diploids consume oxygen at lower rate. Drug treatments revealed major processes accounting for tadpole energy expenditure include cell proliferation, biosynthesis, maintenance plasma membrane potential. While inhibiting proliferation did not abolish consumption difference between triploids, altered cellular biosynthesis or electrical potential did. Combining these results with simple mathematical framework, propose decrease total surface area lowered production activity components including Na

Language: Английский

Citations

18
Genome dilution by cell growth drives starvation-like proteome remodeling in mammalian and yeast cells DOI
Michael C. Lanz, Shuyuan Zhang, Matthew P. Swaffer

et al.

Nature Structural & Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Language: Английский

Citations

9