Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
165, С. 115252 - 115252
Опубликована: Авг. 2, 2023
Intervertebral
disc
degeneration
(IVDD)
leads
to
a
series
of
degenerative
spine
diseases.
Clinical
treatment
IVDD
is
mainly
surgery,
lacking
effective
drugs
alleviate
intervertebral
degeneration.
In
this
study,
we
analysed
the
mRNA
sequencing
dataset
human
tissues
and
revealed
participation
ferroptosis
in
IVDD.
Furthermore,
confirmed
that
TNF-α,
an
important
cytokine
IVDD,
induces
nucleus
pulposus
cells.
Subsequently,
inhibitors
screening
strategy
using
multiple
indicators
was
developed.
Through
screen
various
natural
compounds,
cynarin,
product
enriched
Artichoke,
discovered
inhibit
Cynarin
can
dose-dependently
catabolism
cells,
increase
expression
key
ferroptosis-inhibiting
genes
(GPX4
NRF2),
increment
cellular
Fe2+,
lipid
peroxides,
reactive
oxygen
species.
It
also
prevent
mitochondria
shrinkage,
reduce
cristae
density
ferroptosis,
rat
model.
conclusion,
cynarin
potential
candidate
for
drug
development
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Июль 3, 2024
Ferroptosis
is
a
form
of
non-apoptotic
regulated
cell
death
(RCD)
that
depends
on
iron
and
characterized
by
the
accumulation
lipid
peroxides
to
lethal
levels.
involves
multiple
pathways
including
redox
balance,
regulation,
mitochondrial
function,
amino
acid,
lipid,
glycometabolism.
Furthermore,
various
disease-related
signaling
also
play
role
in
regulating
process
oxidation.
In
recent
years,
with
emergence
concept
ferroptosis
in-depth
study
its
mechanisms,
closely
associated
biological
conditions
related
kidney
diseases,
organ
development,
aging,
immunity,
cancer.
This
article
reviews
development
ferroptosis,
mechanisms
(including
GSH-GPX4,
FSP1-CoQ1,
DHODH-CoQ10,
GCH1-BH4,
MBOAT1/2
pathways),
latest
research
progress
involvement
diseases.
It
summarizes
diseases
within
frameworks
metabolism,
reactive
oxygen
biology,
biology.
The
introduces
key
regulatory
factors
as
well
important
concepts
major
open
questions
natural
compounds.
hoped
future
research,
further
breakthroughs
can
be
made
understanding
regulation
mechanism
utilizing
promote
treatments
for
such
acute
injury(AKI),
chronic
disease
(CKD),
diabetic
nephropathy(DN),
renal
carcinoma.
paves
way
new
approach
prevent,
treat
clinical
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 3, 2025
Early
missed
abortion
is
defined
as
a
pregnancy
of
≤
12
weeks
in
which
there
cessation
life
the
developing
embryo
or
fetus,
leading
to
its
retention
within
uterine
cavity
without
being
spontaneously
expelled
promptly.
This
condition
commonly
observed
and
significantly
impacts
human
reproductive
health.
study
aimed
identify
key
genes
related
ferroptosis
that
could
serve
novel
biomarkers
for
early
abortion.
Findings
from
gene
ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
enrichment
analyses
indicate
correlation
between
iron-
DEFRGS
modules
p53
signaling,
mitophagy-animal,
protein
digestion
absorption
pathways.
An
analysis
protein-protein
interaction
(PPI)
network
was
conducted
on
DEFRGs,
identifying
five
central
(TP53,
EZH2,
TIMP1,
SLC3A2,
GABARAPL2)
using
STRING
Cytohubba
ROC
curves.
The
expression
pivotal
both
missed-abortion
control
groups
verified
by
RT-qPCR.
CIBERSORT
revealed
notable
increase
infiltration
levels
CD8
+
T
lymphocytes
M2
macrophages
among
individuals
group.
Additionally,
ceRNA
constructed
predict
interactions
mRNA,
miRNA,
lncRNA
genes.
However,
interacting
miRNAs
predicted
SLC3A2
miRanda,
miRDB,
TargetScan
databases
were
limited
hsa-miR-661
hsa-miR-4311,
with
no
lncRNAs
found
spongeScan
database.
research
has
identified
be
targeted
detection
management
abortions.
To
explore
the
intergenerational
cardiotoxicity
of
nanoplastics,
maternal
mice
were
exposed
to
60
nm
polystyrene
nanoplastics
(PS-NP)
during
pregnancy
and
lactation.
The
results
showed
that
PS-NP
can
enter
hearts
offspring
induce
myocardial
fiber
arrangement
disorder,
acidophilic
degeneration
cardiomyocytes,
elevated
creatine
kinase
isoenzymes
(CK-MB)
lactate
dehydrogenase
(LDH)
levels
after
exposure
at
100
mg/kg
Mechanistically,
KEGG
analysis
RNA
sequencing
participation
hypoxia-inducible
factor-1
(HIF-1)
ferroptosis
in
PS-NP-induced
cardiotoxicity.
Key
features
ferroptosis,
including
Fe2+
accumulation,
mitochondrial
injury,
oxidative
stress,
GPX4
downregulation,
FTH1,
ACSL4,
SLC7A11
upregulation,
detected.
Furthermore,
treatment
upregulated
expressions
HIF-1α
HO-1,
be
alleviated
by
inhibition
using
si-HIF-1α.
This
study
provided
an
insightful
reference
for
assessment
PS-NP.
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
165, С. 115252 - 115252
Опубликована: Авг. 2, 2023
Intervertebral
disc
degeneration
(IVDD)
leads
to
a
series
of
degenerative
spine
diseases.
Clinical
treatment
IVDD
is
mainly
surgery,
lacking
effective
drugs
alleviate
intervertebral
degeneration.
In
this
study,
we
analysed
the
mRNA
sequencing
dataset
human
tissues
and
revealed
participation
ferroptosis
in
IVDD.
Furthermore,
confirmed
that
TNF-α,
an
important
cytokine
IVDD,
induces
nucleus
pulposus
cells.
Subsequently,
inhibitors
screening
strategy
using
multiple
indicators
was
developed.
Through
screen
various
natural
compounds,
cynarin,
product
enriched
Artichoke,
discovered
inhibit
Cynarin
can
dose-dependently
catabolism
cells,
increase
expression
key
ferroptosis-inhibiting
genes
(GPX4
NRF2),
increment
cellular
Fe2+,
lipid
peroxides,
reactive
oxygen
species.
It
also
prevent
mitochondria
shrinkage,
reduce
cristae
density
ferroptosis,
rat
model.
conclusion,
cynarin
potential
candidate
for
drug
development
