Baicalin attenuates LPS-induced periodontal inflammation response by inhibiting autophagy

BMC Oral Health, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 10, 2025

Periodontal disease causes gradual damage to the periodontal ligament and alveolar bone, ultimately resulting in tooth loss. This condition results from intricate interaction between bacterial infections host's inflammatory responses, driving progression. Autophagy, an essential process for cellular balance under stress, plays a vital role response pathogens. Baicalin (BA), flavonoid extracted Scutellaria baicalensis, is recognized its potent anti-inflammatory effects. However, influence on autophagy health not fully characterized, representing gap therapeutic understanding. study investigates potential of BA by examining regulatory effects inflammation PDLCs. cells (PDLCs) were exposed various concentrations BA, cell proliferation was measured using CCK-8 assay. Anti-inflammatory responses analyzed quantitative real-time PCR (qRT-PCR) enzyme-linked immunosorbent assay (ELISA). Autophagy levels quantified immunofluorescence, transmission electron microscopy (TEM), Western blotting. To identify targets integrated approach combining network pharmacology RNA sequencing (RNA-Seq) employed. These analyses subsequently validated qRT-PCR, molecular docking dynamics simulations. significantly reduced lipopolysaccharide (LPS)-induced PDLCs, as evidenced decrease interleukin (IL)-1β IL-6. RNA-Seq analysis indicated that these associated with autophagy-related processes. Notably, decreased Beclin-1 levels, LC3BII/I ratio, diminished LC3B protein staining, number autophagosomes. Furthermore, triggered activation PI3K/AKT/mTOR pathway, demonstrated increased phosphorylation proteins. acts protective agent against LPS-induced modulating autophagy, positioning it promising candidate future therapies.

Язык: Английский

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Astragalus Polysaccharide Relieves Inflammatory Responses in Guinea Pigs with Allergic Rhinitis via Ameliorating NF-kB-Mediated Treg/Th17 Imbalance

American Journal of Rhinology and Allergy, Год журнала: 2022, Номер 36(5), С. 638 - 648

Опубликована: Май 18, 2022

Allergic rhinitis (AR) is regarded as a prevalent and non-infectious inflammation in nasal mucosa, astragalus polysaccharide (APS) could mitigate inflammation.Herein, this study probed the specific mechanism of APS inflammatory responses AR.Firstly, AR guinea pig models were established through stimulation sensitization ovalbumin (OVA) received treatment. Changes symptoms assessed counting sneezing rubbing times pigs. The change patterns OVA-specific immunoglobulin-E (OVA-sIgE), immunoglobulin-G1 (OVA-sIgG1), tumor necrosis factor (TNF)-α, interleukin (IL)-6 serum identified. Meanwhile, levels IL-17, transforming growth (TGF)-β, IL-10, forkhead box protein P3 (Foxp3) tissues or examined, CD25+Foxp3+Treg CD4+IL17+Th17 cell proportion was detected. Afterwards, nuclear factor-kappa B (NF-kB) expression mucosa examined. Rescue experiments designed to probe role NF-kB overexpression Treg/Th17 imbalance pigs.APS treatment reduced pigs suppressed OVA-sIgE, OVA-sIgG1, TNF-α, IL-6 serum, meanwhile, increased while tissues, dose-dependent manner. highly-expressed down-regulated after facilitated AR.APS via suppressing expression, thereby ameliorating AR.

Язык: Английский

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Integrated Pharmacology Reveals the Molecular Mechanism of Gegen Qinlian Decoction Against Lipopolysaccharide-induced Acute Lung Injury

Frontiers in Pharmacology, Год журнала: 2022, Номер 13

Опубликована: Май 13, 2022

ALI is a severe inflammatory disease of the lungs. In previous studies, we found that GQD was effective against ALI, but specific molecular mechanism still unclear. Therefore, this study to examine effect on LPS-induced rats and underlying mechanisms using multi-omics methods. The results showed significantly improved lung tissue damage, reduced pulmonary edema, inhibited MPO activity, respiratory function in rat. Additionally, levels TNF-α, IL-1β, IL-6 serum BALF. Furthermore, metabolomic analysis inflammation by improving metabolic remodeling. Moreover, transcriptomic activation complement pathway regulated Th17 Treg cells balance. expression C3, C5a, IL-17, promoted TGF-β CYP1A1 at mRNA protein levels. Gut microbial assay treatment increased relative abundance Firmicutes their genera intestinal microbiota, short-chain fatty acids concentration. Overall, treated remodeling, affecting immune-related pathways regulating microbiota. This provides solid scientific basis for promoting clinical use treating ALI.

Язык: Английский

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Baicalin Alleviates Short-Term Lincomycin-Induced Intestinal and Liver Injury and Inflammation in Infant Mice

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(11), С. 6072 - 6072

Опубликована: Май 28, 2022

The adverse effects of short-term megadose antibiotics exposure on the gastrointestinal and liver tissue reactions in young children have been reported. Antibiotic-induced intestinal are usually unpredictable present a poorly understood pathogenesis. It is, therefore, necessary to develop strategies for reducing antibiotics. Studies harm rescue measures from perspective gut-liver system lacking. Here, we demonstrate that lincomycin reduced body weight, disrupted composition gut microbiota morphology, triggered immune-mediated injury inflammation, caused dysfunction, affected lipid metabolism. However, baicalin administration attenuated lincomycin-induced changes. Transcriptome analysis showed improved immunity mice, as evidenced by decreased levels inflammatory cytokines expression genes regulate Th1, Th2, Th17 cell differentiation, inhibited mucin type O-glycan biosynthesis pathways. In addition, function upregulating involved bile acid secretion degradation, downregulating synthesis lincomycin-treated mice. Bile acids can strengthen hepatoenteric circulation. also anti-inflammatory bacteria abundance (Blautia Coprobacillus) pathogenic (Proteobacteria, Klebsiella, Citrobacter) Thus, ameliorate antibiotic-induced its associated complications such disease.

Язык: Английский

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Luteolin restored Treg/Th17 balance to ameliorate allergic rhinitis in a mouse model

Immunopharmacology and Immunotoxicology, Год журнала: 2023, Номер 45(4), С. 461 - 468

Опубликована: Март 22, 2023

Luteolin (LO) has been reported to be a potential drug for allergic rhinitis (AR). This paper explored the mechanism of LO in AR.Mice were treated with ovalbumin (OVA) construct an AR model vivo before or 3-methyladenine (3-MA) treatment. The frequency nasal sneezing was counted. mucosa thickness assessed by hematoxylin-eosin staining assay. levels anti-OVA-immunoglobulin E (IgE)/IgG2a, autophagy-related factors (Beclin1, LC3II/LC3I), and T helper cell 17 (Th17)/regulatory (Treg) markers (interleukin (IL)-17A, retinoic acid receptor-related orphan nuclear receptor γt (RORγt)/IL-10, forkhead box P3 (Foxp3)) detected through enzyme-linked immunosorbent assay, western blot, quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Flow cytometry assay performed test percentage Th17 Treg cells.The frequency, thickness, anti-OVA-IgE, Beclin1, LC3II/LC3I, IL-17A as well RORγt enhanced whereas anti-OVA-IgG2a, IL-10, Foxp3 inhibited mouse OVA-induced AR, which reversed 3-MA treatment.LO restored Treg/Th17 balance ameliorate model.

Язык: Английский

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Circadian rhythm disruption exacerbates Th2‐like immune response in murine allergic airway inflammation

International Forum of Allergy & Rhinology, Год журнала: 2021, Номер 12(5), С. 757 - 770

Опубликована: Ноя. 24, 2021

Chronic jet lag (CJL)-induced circadian rhythm disruption (CRD) is positively correlated with an increased risk of allergic diseases. However, little known about the mechanism involved in rhinitis (AR).Aberrant light/dark cycles-induced CRD mice were randomly divided into negative control (NC) group, AR CRD+NC and CRD+AR group (n = 8/group). After ovalbumin (OVA) challenge, nasal symptom scores recorded. The expression Occludin ZO-1 both mucosa lung tissues was detected by reverse transcription-quantitative polymerase chain reaction (RT-PCR) immunohistochemical staining. level OVA-specific immunoglobulin E (sIgE) T-helper (Th)-related cytokines plasma measured enzyme-linked immunosorbent assay (ELISA), proportion Th1, Th2, Th17, regulatory T cell (Treg) splenocytes evaluated flow cytometry.The score significantly higher than those respect to eosinophil infiltration, mast degranulation, goblet hyperplasia. lower group. Furthermore, Th2 Th17 counts from OVA-sIgE, interleukin 4 (IL-4), IL-6, IL-13, IL-17A levels whereas Th1 Treg count interferon γ (IFN-γ) decreased group.CRD experimentally mimicked CJL human activities, could exacerbate local systemic reactions mice, partially through decreasing respiratory increasing Th2-like immune response splenocytes.

Язык: Английский

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Allergen induces CD11c+ dendritic cell autophagy to aggravate allergic rhinitis through promoting immune imbalance

International Immunopharmacology, Год журнала: 2022, Номер 106, С. 108611 - 108611

Опубликована: Фев. 11, 2022

Язык: Английский

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Lidocaine relieves murine allergic rhinitis by regulating the NF‑κB and p38 MAPK pathways

Experimental and Therapeutic Medicine, Год журнала: 2022, Номер 23(3)

Опубликована: Янв. 5, 2022

Allergic rhinitis (AR) is one of the most common chronic inflammatory diseases and its main feature nasal mucositis. It has been recently revealed that lidocaine demonstrates optimal effects in treatment various diseases. However, a limited number studies have examined association between AR. In present study, AR mouse model was established to explore further analyze molecular mechanism. Subsequently, different concentrations were provided animals by intranasal administration series indices assessed. The data indicated frequencies sneezing nose rubbing suppressed following an increase concentration. cells measured. Wright's‑Giemsa staining results significantly decreased numbers leukocytes, eosinophils, neutrophils lymphocytes lavage fluid (NLF) mice. addition, expression levels ovalbumin (OVA)‑specific immunoglobulin E (IgE), leukotriene C4 (LTC4) certain factors assessed ELISA. Lidocaine reduced OVA‑specific IgE LTC4 NLF plasma derived from also IL‑4, IL‑5, IL‑13, IL‑17 TNF‑α. caused upregulation IFN‑γ IL‑2 levels. western blot analysis phosphorylated (p)‑p38 p‑p65 Collectively, NF‑κB p38 MAPK signaling pathways involved lidocaine‑mediated relief order verify mice, inhibitor IMD‑0354 SB 203580 on these two compounds exhibited similar inhibitory mice as those noted with use lidocaine. These findings suggested represented novel therapeutic agent for

Язык: Английский

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Sanfeng Tongqiao Dripping Pills alleviate House Dust Mite-induced allergic rhinitis in mice by inhibiting Th2 differentiation and repairing the nasal epithelial barrier

Phytomedicine, Год журнала: 2024, Номер 132, С. 155899 - 155899

Опубликована: Июль 18, 2024

Язык: Английский

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Recent advances in understanding the effects of T lymphocytes on mucosal barrier function in allergic rhinitis

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Сен. 12, 2023

Allergic rhinitis is a non-infectious chronic inflammatory disease of the nasal mucosa that affects T cells and their cytokines. play significant roles in development allergic diseases by orchestrating mechanisms underlying innate adaptive immunity. Although many studies on have focused helper cells, molecular makeup, pathogenesis-related transduction pathways, pathological not yet been completely explored. Recent suggested cell status may an important role interaction between mucosal barrier rhinitis. This study aimed to explore interactions barriers review therapeutic modalities pertinent biological agents involving cells.

Язык: Английский

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