Irigenin Alleviates Blue Light‐Induced Retinal Damage by Upregulating Antioxidative Defense System via Nrf2 Pathway In Vivo and In Vitro
Environmental Toxicology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 27, 2025
ABSTRACT
This
research
aimed
to
assess
the
potential
of
irigenin
attenuate
blue
light
(BL)‐induced
apoptosis
in
human
adult
retinal
pigment
epithelial
(hARPE‐19)
cells
loaded
with
N
‐retinylidene‐
‐retinylethanolamine
(A2E,
DA50062).
Furthermore,
study
investigated
associated
molecular
mechanisms.
Cell
viability
was
assessed
using
MTT
assay,
and
flow
cytometry
employed
evaluate
reactive
oxygen
species
(ROS)
production,
alterations
mitochondrial
membrane
potential,
cytochrome
c
release.
Lipid
peroxidation
levels,
as
well
activities
superoxide
dismutase
(SOD),
catalase
(CAT),
glutathione
peroxidase
(GSH‐Px),
caspase
enzymes,
were
quantified
commercially
available
assay
kits.
Bcl‐2,
Bax,
heme
oxygenase‐1
(HO‐1),
nuclear
factor
erythroid
2‐related
2
(Nrf2)
expression
through
western
blotting.
Moreover,
animal
experiments
performed
BL‐induced
damage.
The
results
revealed
that
protected
against
cytotoxicity
DA50062‐laden
hARPE‐19
cells.
upregulated
Bcl‐2
downregulated
Bax
BL‐exposed
Hence,
prevented
release
inhibited
caspase‐3
caspase‐9
activation
Irigenin
also
effectively
lipid
ROS
production
Notably,
Nrf2
expression,
which,
turn,
several
antioxidative
defense
system,
such
SOD,
CAT,
GSH‐Px,
HO‐1
Animal
studies
showed
damage,
indicated
by
increased
thickness
outer
inner
layers
irigenin‐treated
groups
compared
untreated
controls.
Taken
together,
suggest
inhibits
intrinsic
apoptotic
pathway
activating
Nrf2‐mediated
system.
Язык: Английский
MicroRNA transcriptome analysis reveals the immune regulatory mechanism of Crassostrea hongkongesis against Vibrio harveyi infection
Fish & Shellfish Immunology,
Год журнала:
2024,
Номер
145, С. 109354 - 109354
Опубликована: Янв. 1, 2024
Язык: Английский
Programmed cell death: molecular mechanisms, biological functions, diseases, and therapeutic targets
MedComm,
Год журнала:
2024,
Номер
5(12)
Опубликована: Ноя. 28, 2024
Programmed
cell
death
represents
a
precisely
regulated
and
active
cellular
demise,
governed
by
complex
network
of
specific
genes
proteins.
The
identification
multiple
forms
programmed
has
significantly
advanced
the
understanding
its
intricate
mechanisms,
as
demonstrated
in
recent
studies.
A
thorough
grasp
these
processes
is
essential
across
various
biological
disciplines
study
diseases.
Nonetheless,
despite
notable
progress,
exploration
relationship
between
disease,
well
clinical
application,
are
still
nascent
stage.
Therefore,
further
development
corresponding
therapeutic
methods
strategies
holds
substantial
potential.
Our
review
provides
detailed
examination
primary
mechanisms
behind
apoptosis,
autophagy,
necroptosis,
pyroptosis,
ferroptosis.
Following
this,
discussion
delves
into
functions
diseases
associated
dysregulated
death.
Finally,
we
highlight
existing
potential
targets
focused
on
cancers
neurodegenerative
This
aims
to
summarize
latest
insights
from
more
reliable
approach
for
transformation.
Язык: Английский
Safe Concentration, Unsafe Effects: Impact of BPA on Antioxidant Function in the Hepatopancreas and Ovarian Gene Expression in Oriental River Prawns (Macrobrachium nipponense)
Aquatic Toxicology,
Год журнала:
2024,
Номер
276, С. 107103 - 107103
Опубликована: Сен. 16, 2024
Язык: Английский
Safe Concentration, Unsafe Effects: Impact of Bpa on Antioxidant Function in the Hepatopancreas and Ovarian Gene Expression in Oriental River Prawns ( Macrobrachium Nipponense )
Опубликована: Янв. 1, 2024
Язык: Английский
Insights into Structure and Function of Growth Arrest Specific 2 (GAS2)
Journal of Cancer,
Год журнала:
2024,
Номер
16(1), С. 146 - 156
Опубликована: Дек. 11, 2024
Growth
arrest
specific
2
(GAS2)
is
a
microfilament-associated
protein,
which
widely
distributed
in
human
tissues.
It
exerts
pivotal
influence
on
various
cellular
processes,
including
cytoskeletal
regulation,
cell
cycle
progression,
apoptosis,
and
senescence.
GAS2
has
dual
function
cancer
growth:
the
one
hand,
it
enhances
sensitivity
of
cells
to
chemoradiotherapy
prevents
malignant
transformation
normal
cells;
but
other
maintains
growth
cells.
regulates
activity
Calpain-2,
calcium-dependent
protease,
by
acting
as
an
endogenous
inhibitor
enzyme.
The
N-terminus
binds
while
its
C-terminus
acts
protease
Calpain-2.
functional
outcome
highly
dependent
substrates
Calpain-2
environment,
particularly
within
tumor
Despite
garnering
increasing
attention
growing
body
related
research,
systematic
review
remains
absent.
This
aims
elucidate
structural
aspects
GAS2,
with
particular
emphasis
implications
cancer.
By
comprehensively
detailing
role
research
progress
malignancies,
this
endeavors
furnish
novel
insights
for
enhancing
therapeutic
strategies
against
diseases,
cancers.
Язык: Английский
Humic Substances Suppresses the Proliferation of TC-1 Cells, the Lung Cancer Cell
Journal of Experimental & Biomedical Sciences/Biomedical Science Letters,
Год журнала:
2023,
Номер
29(4), С. 280 - 286
Опубликована: Дек. 31, 2023
In
humic
substances,
fulvic
acid
(FA)
is
a
subclass
of
diverse
compounds
known
as
which
are
byproducts
organic
degradation
from
microorganisms.FA
can
suppress
the
proliferation
tumor
cells.Despite
numerous
studies,
exact
mechanism
for
various
effects
FA
not
clearly
understood.Based
on
results
demonstrating
anti-proliferation
human
cancer,
we
investigated
whether
has
similar
lung
cancer
in
this
study.Firstly,
anti-cancer
effect
pulmonary
epithelial
cell
lines
(TC-1
cells)
was
examined
by
confirming
its
inhibitory
TC-1
cells.TC-1
reduced
dose-dependent
and
time-dependent
manner.After
24
hours
treatment,
morphological
changes
such
volume
decrease,
non-adherence
increased
number
apoptotic
cells
were
observed.In
addition,
induced
DNA
ladder
pattern
fragments
cells.In
intracellular
regulatory
pathway
FA,
confirmed
that
reduction
anti-apoptotic
protein,
Bcl-2
protein
levels.These
indicate
anticancer
inducing
pathway.Further
research
will
be
basic
data
development
potential
drugs.
Язык: Английский