MitoMAMMAL: a genome scale model of mammalian mitochondria predicts cardiac and BAT metabolism DOI Creative Commons
Stephen P. Chapman,

Theo Brunet,

Arnaud Mourier

и другие.

Bioinformatics Advances, Год журнала: 2024, Номер 5(1)

Опубликована: Ноя. 5, 2024

Abstract Motivation Mitochondria are essential for cellular metabolism and inherently flexible to allow correct function in a wide range of tissues. Consequently, dysregulated mitochondrial affects different tissues ways leading challenges understanding the pathology diseases. System-level metabolic modelling is useful studying tissue-specific metabolism, yet despite mouse being common model organism research, no specific currently available. Results Building upon similarity between human we present mitoMammal, genome-scale that contains gene-product reaction rules. MitoMammal able metabolism. To demonstrate this, using an adapted E-Flux algorithm, integrated proteomic data from mitochondria isolated cardiomyocytes brown adipocyte tissue, as well transcriptomic vitro differentiated adipocytes modelled context flux balance analysis. In all three simulations, mitoMammal made mostly accurate, some novel predictions relating energy adipocytes. This demonstrates its usefulness research cardiac disease diabetes both contexts. Availability implementation The Jupyter Notebook available at: https://gitlab.com/habermann_lab/mitomammal.

Язык: Английский

Insights on post-translational modifications in fatty liver and fibrosis progression DOI

C. Nageswara Raju,

Kavitha Sankaranarayanan

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2025, Номер 1871(3), С. 167659 - 167659

Опубликована: Янв. 7, 2025

Язык: Английский

Процитировано

1
Lactate-induced protein lactylation in cancer: functions, biomarkers and immunotherapy strategies DOI Creative Commons
Wenjuan Wang, Hong Wang, Qi Wang

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 10, 2025

Lactate, long viewed as a byproduct of glycolysis and metabolic waste. Initially identified within the context yogurt fermentation, lactate's role extends beyond culinary applications to its significance in biochemical processes. Contemporary research reveals that lactate functions not merely terminal product but also nexus for initiating physiological pathological responses body. Lysine lactylation (Kla), novel post-translational modification (PTM) proteins, has emerged pivotal mechanism by which exerts regulatory influence. This epigenetic potential alter gene expression patterns, thereby impacting Increasing evidence indicates correlation between adverse prognosis various malignancies. Consequently, this review article aims encapsulate proteins interact with lactate, elucidate tumorigenesis progression, explore therapeutic targets afforded modulation lactylation. The objective is clarify oncogenic provide strategic framework future directions burgeoning field.

Язык: Английский

Процитировано

1
Total Saponins from Panax japonicus Reduced Lipid Deposition and Inflammation in Hepatocyte via PHD2 and Hepatic Macrophage-derived Exosomal miR-463-5p DOI

Jingjie Yang,

Yibei Gui,

Ying Zheng

и другие.

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119376 - 119376

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Conjugated linoleic acid (CLA) reduces intestinal fatty acid uptake and chylomicron formation in HFD-fed mice associated with the inhibition of DHHC7-mediated CD36 palmitoylation and the downstream ERK pathway DOI
Fenglin Zhang, Yiming Fu, Junfeng Wang

и другие.

Food & Function, Год журнала: 2024, Номер 15(9), С. 5000 - 5011

Опубликована: Янв. 1, 2024

The anti-obesity effect of conjugated linoleic acid (CLA) has been well elucidated, but whether CLA affects fat deposition by regulating intestinal dietary absorption remains largely unknown. Thus, this study aimed to investigate the effects on fatty uptake and chylomicron formation explore possible underlying mechanisms. We found that supplementation reduced in HFD (high diet)-fed mice accompanied decreased serum TG level, increased fecal lipids expression ApoB48 MTTP. Correspondingly, c9, t11-CLA, not t10, c12-CLA induced reduction content PA (palmitic acid)-treated MODE-K cells. In mechanism uptake, t11-CLA inhibited binding CD36 with palmitoyltransferase DHHC7, thus leading decreases palmitoylation level localization cell membrane PA-treated formation, CD36/FYN/LYN complex activation ERK pathway vivo verification, DHHC7-mediated total suppressed jejunum HFD-fed mice. Altogether, these data showed associated inhibition downstream pathway.

Язык: Английский

Процитировано

4
Conjugated Linoleic Acid (CLA) Mitigates High-Fat Diet (HFD)-Induced Mammary Gland Development Impairment of Pubertal Mice via Regulating CD36 Palmitoylation and Downstream JNK–ERK Pathway DOI
Fenglin Zhang, Yiming Fu,

Jisong Zheng

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 3, 2025

Conjugated linoleic acid (CLA) is known for antiobesity. However, the role of CLA in regulating high-fat diet (HFD)-impaired pubertal mammary gland development remains undefined. Here, female mice and HC11 cells were treated with HFD or palmitic (PA), supplemented without CLA, respectively. We found that prevented impaired exposed to HFD. In vitro, c9, t11-CLA, but not t10, c12-CLA, promoted PA-suppressed proliferation, accompanied by hindered CD36 palmitoylation localization on plasma membrane. Moreover, t11-CLA reduced formation CD36/Fyn/Lyn complex inhibited JNK pathway while activated ERK PA-treated HC11. mechanism, activation inhibition abolished t11-CLA-stimulated proliferation vivo verification, total cell membrane palmitoylation, suppressed CD36/FYN/LYN complex, HFD-fed mice. conclusion, mitigated HFD-impaired via downstream JNK–ERK pathway. These data suggested potential application ameliorating obesity-impaired development.

Язык: Английский

Процитировано

0
TaoHeChengQi Decotion Alleviate Chronic renal failure via Regulation of PHD2/UCP1 and RIPK3/AKT/TGF-β Pathway DOI
Wei Liu, Cheng Hu,

Xiaojing Qian

и другие.

Phytomedicine, Год журнала: 2025, Номер 141, С. 156548 - 156548

Опубликована: Март 13, 2025

Язык: Английский

Процитировано

0
PHD1-3 oxygen sensors in vivo—lessons learned from gene deletions DOI Creative Commons
Agnieszka E. Jucht, Carsten C. Scholz

Pflügers Archiv - European Journal of Physiology, Год журнала: 2024, Номер 476(9), С. 1307 - 1337

Опубликована: Март 21, 2024

Oxygen sensors enable cells to adapt limited oxygen availability (hypoxia), affecting various cellular and tissue responses. Prolyl-4-hydroxylase domain 1-3 (PHD1-3; also called Egln1-3, HIF-P4H 1-3, HIF-PH 1-3) proteins belong the Fe

Язык: Английский

Процитировано

1
The P124A mutation of SRP14 alters its migration on SDS-PAGE without impacting its function DOI Creative Commons

Yaofu Liu,

Jin‐Qiu Zhou

Acta Biochimica et Biophysica Sinica, Год журнала: 2024, Номер 56(2), С. 315 - 322

Опубликована: Янв. 15, 2024

SRP14 is a crucial protein subunit of the signal recognition particle (SRP), ribonucleoprotein complex essential for co-translational translocation to endoplasmic reticulum. During our investigation expression across diverse cell lines, we observe variations in its migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with some cells exhibiting slower and others migrating faster. However, cause this phenomenon remains elusive. Our research rules out alternative splicing as and, instead, identifies presence P124A mutation (SRP14

Язык: Английский

Процитировано

0
MitoMAMMAL: a genome scale model of mammalian mitochondria predicts cardiac and BAT metabolism DOI Creative Commons
Stephen P. Chapman,

Theo Brunet,

Arnaud Mourier

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 26, 2024

Abstract Mitochondria perform several essential functions in order to maintain cellular homeostasis and mitochondrial metabolism is inherently flexible allow correct function a wide range of tissues. Dysregulated can therefore affect different tissues ways which presents experimental challenges understanding the pathology diseases. System-level metabolic modelling useful gaining in-depth insights into tissue-specific metabolism, yet despite mouse being common model organism used research, there currently no specific available. In this work, building upon similarity between human we have created mitoMammal, genome-scale that contains gene-product reaction rules. MitoMammal able metabolism. To demonstrate feature, using an adapted E-Flux2 algorithm, first integrated proteomic data extracted from mitochondria isolated cardiomyocytes brown adipocyte tissue. We then transcriptomic vitro differentiated adipose cells modelled context flux balance analysis. all three simulations, mitoMammal made mostly accurate, some novel predictions relating energy adipocytes. This demonstrates its usefulness research cardiac disease diabetes both contexts.

Язык: Английский

Процитировано

0
MitoMAMMAL: a genome scale model of mammalian mitochondria predicts cardiac and BAT metabolism DOI Creative Commons
Stephen P. Chapman,

Theo Brunet,

Arnaud Mourier

и другие.

Bioinformatics Advances, Год журнала: 2024, Номер 5(1)

Опубликована: Ноя. 5, 2024

Abstract Motivation Mitochondria are essential for cellular metabolism and inherently flexible to allow correct function in a wide range of tissues. Consequently, dysregulated mitochondrial affects different tissues ways leading challenges understanding the pathology diseases. System-level metabolic modelling is useful studying tissue-specific metabolism, yet despite mouse being common model organism research, no specific currently available. Results Building upon similarity between human we present mitoMammal, genome-scale that contains gene-product reaction rules. MitoMammal able metabolism. To demonstrate this, using an adapted E-Flux algorithm, integrated proteomic data from mitochondria isolated cardiomyocytes brown adipocyte tissue, as well transcriptomic vitro differentiated adipocytes modelled context flux balance analysis. In all three simulations, mitoMammal made mostly accurate, some novel predictions relating energy adipocytes. This demonstrates its usefulness research cardiac disease diabetes both contexts. Availability implementation The Jupyter Notebook available at: https://gitlab.com/habermann_lab/mitomammal.

Язык: Английский

Процитировано

0