Next-Generation Breeding Strategies for Climate-Ready Crops

Frontiers in Plant Science, Год журнала: 2021, Номер 12

Опубликована: Июль 21, 2021

Climate change is a threat to global food security due the reduction of crop productivity around globe. Food matter concern for stakeholders and policymakers as population predicted bypass 10 billion in coming years. Crop improvement via modern breeding techniques along with efficient agronomic practices innovations microbiome applications, exploiting natural variations underutilized crops an excellent way forward fulfill future requirements. In this review, we describe next-generation tools that can be used increase production by developing climate-resilient superior genotypes cope challenges security. Recent genomic-assisted (GAB) strategies allow construction highly annotated pan-genomes give snapshot full landscape genetic diversity (GD) recapture lost gene repertoire species. Pan-genomes provide new platforms exploit these unique genes or variation optimizing programs. The advent clustered regularly interspaced short palindromic repeat/CRISPR-associated (CRISPR/Cas) systems, such prime editing, base de nova domestication, has institutionalized idea genome editing revamped improvement. Also, availability versatile Cas orthologs, including Cas9, Cas12, Cas13, Cas14, improved efficiency. Now, CRISPR/Cas systems have numerous applications research successfully edit major develop resistance against abiotic biotic stress. By adopting high-throughput phenotyping approaches big data analytics like artificial intelligence (AI) machine learning (ML), agriculture heading toward automation digitalization. integration speed genomic phenomic rapid identifications ultimately accelerate addition, multidisciplinary open exciting avenues climate-ready

Язык: Английский

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Features and applications of haplotypes in crop breeding

Communications Biology, Год журнала: 2021, Номер 4(1)

Опубликована: Ноя. 4, 2021

Abstract Climate change with altered pest-disease dynamics and rising abiotic stresses threatens resource-constrained agricultural production systems worldwide. Genomics-assisted breeding (GAB) approaches have greatly contributed to enhancing crop efficiency delivering better varieties. Fast-growing capacity affordability of DNA sequencing has motivated large-scale germplasm projects, thus opening exciting avenues for mining haplotypes applications. This review article highlights ways mine apply them complex trait dissection in GAB including haplotype-GWAS, haplotype-based breeding, haplotype-assisted genomic selection. Improvement strategies that efficiently deploy superior hasten progress will be key safeguarding global food security.

Язык: Английский

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Molecular Markers and Their Applications in Marker-Assisted Selection (MAS) in Bread Wheat (Triticum aestivum L.)

Agriculture, Год журнала: 2023, Номер 13(3), С. 642 - 642

Опубликована: Март 8, 2023

As one of the essential cereal crops, wheat provides 20% calories and proteins consumed by humans. Due to population expansion, dietary shift climate change, it is challenging for breeders develop new varieties meeting production requirements. Marker-assisted selection (MAS) has distinct advantages over conventional in plant breeding, such as being time-saving, cost-effective goal-oriented. This review makes attempts give a description different molecular markers: sequence tagged site (STS), simple repeat (SSR), genotyping sequencing (GBS), single nucleotide polymorphism (SNP) arrays, exome capture, Kompetitive Allele Specific PCR (KASP), cleaved amplified polymorphic (CAPS), semi-thermal asymmetric reverse (STARP) target (GBTS). We also summarize some quantitative trait loci (QTL)/genes well their linked markers, which are potentially useful MAS. paper updated information on markers critical traits potential applications breeding programs.

Язык: Английский

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Trends in plant research using molecular markers

Planta, Год журнала: 2017, Номер 247(3), С. 543 - 557

Опубликована: Дек. 14, 2017

Язык: Английский

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Can genomics deliver climate-change ready crops?

Current Opinion in Plant Biology, Год журнала: 2018, Номер 45, С. 205 - 211

Опубликована: Апрель 21, 2018

Development of climate resilient crops with accelerating genetic gains in will require integration different disciplines/technologies, to see the impact farmer's field. In this review, we summarize how are utilizing our germplasm collections identify superior alleles/haplotypes through NGS based sequencing approaches and genomics-enabled technologies together precise phenotyping being used crop breeding. Pre-breeding genomics-assisted breeding contributing more efficient development climate-resilient crops. It is anticipated that several disciplines/technologies result delivery change ready less time.

Язык: Английский

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Genomic resources in plant breeding for sustainable agriculture

Journal of Plant Physiology, Год журнала: 2020, Номер 257, С. 153351 - 153351

Опубликована: Дек. 17, 2020

Climate change during the last 40 years has had a serious impact on agriculture and threatens global food nutritional security. From over half million plant species, cereals legumes are most important for Although systematic breeding relatively short history, conventional coupled with advances in technology crop management strategies increased yields by 56 % globally between 1965−85, referred to as Green Revolution. Nevertheless, demand food, feed, fiber, fuel necessitates need break existing yield barriers many plants. In first decade of 21st century we witnessed rapid discovery, transformative technological development declining costs genomics technologies. second decade, field turned towards making sense vast amount genomic information subsequently moved accurately predicting gene-to-phenotype associations tailoring plants climate resilience this review focus resources, genome germplasm sequencing, sequencing-based trait mapping, genomics-assisted approaches aimed at developing biotic stress resistant, abiotic tolerant high nutrition varieties six major (rice, maize, wheat, barley, sorghum pearl millet), (soybean, groundnut, cowpea, common bean, chickpea pigeonpea). We further provide perspective way forward use including marker-assisted selection, backcrossing, haplotype based prediction machine learning artificial intelligence, speed approaches. The overall goal is accelerate genetic gains deliver resilient sustainable agriculture.

Язык: Английский

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Development of multiple SNP marker panels affordable to breeders through genotyping by target sequencing (GBTS) in maize

Molecular Breeding, Год журнала: 2019, Номер 39(3)

Опубликована: Фев. 20, 2019

Язык: Английский

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Fine mapping and gene cloning in the post-NGS era: advances and prospects

Theoretical and Applied Genetics, Год журнала: 2020, Номер 133(5), С. 1791 - 1810

Опубликована: Фев. 10, 2020

Abstract Improvement in traits of agronomic importance is the top breeding priority crop improvement programs. Majority these show complex quantitative inheritance. Identification trait loci (QTLs) followed by fine mapping QTLs and cloning candidate genes/QTLs central to analysis. Advances genomic technologies revolutionized our understanding genetics traits, regions associated with were employed marker-assisted or QTLs/genes. Next-generation sequencing (NGS) have enabled genome-wide methodologies for development ultra-high-density genetic linkage maps different crops, thus allowing placement within few kbs genomes. In this review, we compare marker systems used QTL pre- post-NGS era. We then discuss how NGS platforms combination advanced experimental designs improved analysis mapping. opine that efficient genotyping/sequencing assays may circumvent need cumbersome procedures earlier A deeper architectures agricultural significance will be crucial accelerate improvement.

Язык: Английский

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From markers to genome-based breeding in wheat

Theoretical and Applied Genetics, Год журнала: 2019, Номер 132(3), С. 767 - 784

Опубликована: Янв. 23, 2019

Язык: Английский

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Genome-wide association mapping of black point reaction in common wheat (Triticum aestivum L.)

BMC Plant Biology, Год журнала: 2017, Номер 17(1)

Опубликована: Ноя. 23, 2017

Black point is a serious threat to wheat production and can be managed by host resistance. Marker-assisted selection (MAS) has the potential accelerate genetic improvement of black resistance in breeding. We performed genome-wide association study (GWAS) using high-density 90 K 660 single nucleotide polymorphism (SNP) assays better understand basis identify associated molecular markers. reactions were evaluated 166 elite cultivars five environments. Twenty-five unique loci identified on chromosomes 2A, 2B, 3A, 3B (2), 3D, 4B 5A (3), 5B 6A, 6B, 6D, 7A (5), 7B 7D respectively, explaining phenotypic variation ranging from 7.9 18.0%. The highest number was detected A genome (11), followed B (10) D (4) genomes. Among these, 13 two or more Seven coincided with known genes quantitative trait locus (QTL), whereas other 18 potentially novel loci. Linear regression showed clear dependence scores favorable alleles, suggesting that QTL pyramiding will an effective approach increase In silico analysis sequences resistance-associated SNPs 6 possibly involved oxidase, signal transduction stress as candidate reaction. SNP markers significantly accessions larger alleles used further enhance This provides new insights into architecture

Язык: Английский

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