Horticultural Plant Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 1, 2024
Tomato
is
an
important
economic
crop
all
over
the
world.
Volatile
flavors
in
tomato
fruit
are
key
factors
influencing
consumer
liking
and
commercial
quality.
However,
regulatory
mechanism
controlling
volatile
of
tomatoes
still
not
clear.
Here,
we
integrated
metabolome
transcriptome
to
explore
flavor
formation,
using
wild
cultivated
with
significant
differences
flavors.
A
total
35
compounds
were
identified,
based
on
solid
phase
microextraction-gas
chromatography-mass
spectrometry
(SPME-GC-MS).
The
content
volatiles,
affecting
flavor,
significantly
increased
transition
from
breaker
red
ripe
stage.
Moreover,
volatiles
was
much
higher
than
that
tomatoes.
variations
clustered
into
10
groups
by
hierarchical
cluster
Pearson
correlation
analysis
(PCC).
also
patterned
groups,
both
mature
green
stage
Combining
same
developmental
fruits
co-expression
analysis,
found
expression
level
1
182
genes
highly
correlated
compounds,
thereby
constructing
two
pathways
One
pathway
tetrahydrothiazolidine
N-hydroxylase
(SlTNH1)-dependent,
which
regulated
transcription
(TFs)
bHLH
AP2/ERF
families,
synthesis
2-isobutylthiazole
amino
acid
metabolism.
other
lipoxygenase
(SlLOX)-dependent,
one
TF
HD-Zip
family,
hexanal
(Z)-2-heptenal
fatty
Dual-luciferase
assay
confirmed
binding
their
structural
genes.
findings
this
study
provide
new
insights
formation
fruit,
can
be
useful
for
improvement.
New Phytologist,
Год журнала:
2023,
Номер
238(5), С. 2064 - 2079
Опубликована: Фев. 27, 2023
Kiwifruit
(Actinidia
chinensis)
is
one
of
the
popular
fruits
world-wide,
and
its
quality
mainly
determined
by
key
metabolites
(sugars,
flavonoids,
vitamins).
Previous
works
on
kiwifruit
are
mostly
done
via
a
single
omics
approach
or
involve
only
limited
metabolites.
Consequently,
dynamic
metabolomes
during
development
ripening
underlying
regulatory
mechanisms
poorly
understood.
In
this
study,
using
high-resolution
metabolomic
transcriptomic
analyses,
we
investigated
metabolic
landscapes
at
11
different
developmental
stages
revealed
parallel
classification
515
their
co-expressed
genes
into
10
distinct
vs
gene
modules
(MM
GM).
Through
integrative
bioinformatics
coupled
with
functional
genomic
assays,
constructed
global
map
uncovered
essential
transcriptional
networks
for
all
major
changes
that
occurred
throughout
growth
cycle.
Apart
from
known
MM
GM
such
as
soluble
sugars,
identified
novel
transcription
factors
regulate
accumulation
procyanidins,
vitamin
C,
other
important
Our
findings
thus
shed
light
network
provide
valuable
resource
designed
improvement
quality.
Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
66(4), С. 810 - 823
Опубликована: Фев. 20, 2024
ABSTRACT
The
structural
and
functional
diversity
of
plant
metabolites
is
largely
created
via
chemical
modification
a
basic
backbone.
However,
metabolite
modifications
in
plants
have
still
not
been
thoroughly
investigated
by
metabolomics
approaches.
In
this
study,
widely
targeted
modificomics
(WTMM)
strategy
was
developed
based
on
ultra‐high
performance
liquid
chromatography‐quadrupole‐linear
ion
trap
(UHPLC‐Q‐Trap)
UHPLC‐Q‐Exactive‐Orbitrap
(UHPLC‐QE‐Orbitrap),
which
greatly
improved
the
detection
sensitivity
efficiency
identification
modified
metabolites.
A
study
carried
out
using
tomato
as
model,
over
34,000
signals
with
MS2
information
were
obtained
from
approximately
232
neutral
loss
transitions.
Unbiased
profiling
also
performed
utilizing
high‐resolution
mass
spectrometry
data
to
annotate
total
2,118
125
types;
these,
165
identified
study.
Next,
WTMM
database
used
assess
diseased
tissues
29
biomarkers
analyzed.
summary,
only
capable
large‐scale
quantitative
analysis
plant‐modified
plants,
but
can
be
for
biomarker
development.
Globally,
medicinal
plant
natural
products
(PNPs)
are
a
major
source
of
substances
used
in
traditional
and
modern
medicine.
As
we
human
race
face
the
tremendous
public
health
challenge
posed
by
emerging
infectious
diseases,
antibiotic
resistance
surging
drug
prices
etc.,
harnessing
healing
power
plants
gifted
from
mother
nature
is
more
urgent
than
ever
helping
us
survive
future
sustainable
way.
PNP
research
efforts
pre-genomic
era
focus
on
discovering
bioactive
molecules
with
pharmaceutical
activities,
identifying
individual
genes
responsible
for
biosynthesis.
Critically,
systemic
biological,
multi-
inter-disciplinary
approaches
integrating
interrogating
all
accessible
data
genomics,
metabolomics,
structural
biology,
chemical
informatics
necessary
to
accelerate
full
characterization
biosynthetic
regulatory
circuitry
producing
PNPs
plants.
In
this
review,
attempt
provide
brief
update
current
focusing
how
different
state-of-the-art
biotechnologies
facilitate
their
discovery,
molecular
basis
biosynthesis,
as
well
synthetic
biology.
Finally,
humbly
foresight
trend
understanding
biology
coming
decades.
The Plant Cell,
Год журнала:
2023,
Номер
36(2), С. 298 - 323
Опубликована: Окт. 17, 2023
Abstract
The
high-yielding
Green
Revolution
varieties
of
cereal
crops
are
characterized
by
a
semidwarf
architecture
and
lodging
resistance.
Plant
height
is
tightly
regulated
the
availability
phosphate
(Pi),
yet
underlying
mechanism
remains
obscure.
Here,
we
report
that
rice
(Oryza
sativa)
R2R3-type
Myeloblastosis
(MYB)
transcription
factor
MYB110
Pi-dependent
negative
regulator
plant
height.
direct
target
PHOSPHATE
STARVATION
RESPONSE
2
(OsPHR2)
regulates
OsPHR2-mediated
inhibition
Inactivation
increased
culm
diameter
bending
resistance,
leading
to
enhanced
resistance
despite
Strikingly,
grain
yield
myb110
mutants
was
elevated
under
both
high-
low-Pi
regimes.
Two
divergent
haplotypes
based
on
single
nucleotide
polymorphisms
in
putative
promoter
corresponded
with
its
transcript
levels
response
Pi
availability.
Thus,
fine-tuning
expression
may
be
potent
strategy
for
further
increasing
crop
varieties.
The Plant Cell,
Год журнала:
2024,
Номер
36(9), С. 3362 - 3377
Опубликована: Май 27, 2024
Abstract
The
phytohormone
jasmonate
(JA)
plays
a
central
role
in
plant
defenses
against
biotic
stressors.
However,
our
knowledge
of
the
JA
signaling
pathway
rice
(Oryza
sativa)
remains
incomplete.
Here,
we
integrated
multiomic
data
from
three
tissues
to
characterize
functional
modules
involved
organizing
JA-responsive
genes.
In
core
regulatory
sector,
MYC2
transcription
factor
transcriptional
cascades
are
conserved
different
species
but
with
distinct
regulators
(e.g.
bHLH6
rice),
which
genes
early
expressed
across
all
tissues.
feedback
also
regulates
expression
repressor
and
catabolic
genes,
providing
negative
that
truncates
duration
responses.
For
example,
MYC2-regulated
NAC
(NAM,
ATAF1/2,
CUC2)
NAC1,
NAC3,
NAC4
encode
proteins
repress
herbivore
resistance.
tissue-specific
many
late-expressed
associated
biosynthesis
specialized
metabolites
mediate
particular
defensive
functions.
terpene
synthase
gene
TPS35
is
specifically
induced
leaf
sheath
functions
defense
oviposition
by
brown
planthoppers
attraction
this
herbivore's
natural
enemies.
Thus,
characterizing
core,
tissue-specific,
sectors
JA-elicited
responses,
work
provides
valuable
resource
for
future
discoveries
key
components
important
crop.
Advanced Biotechnology,
Год журнала:
2024,
Номер
2(3)
Опубликована: Июнь 17, 2024
Microorganisms
in
eutrophic
water
play
a
vital
role
nitrogen
(N)
removal,
which
contributes
significantly
to
the
nutrient
cycling
and
sustainability
of
ecosystems.
However,
mechanisms
underlying
interactions
adaptation
strategies
N
removal
microorganisms
ecosystems
remain
unclear.
We
thus
analyzed
field
sediments
collected
from
freshwater
ecosystem,
enriched
microorganisms,
examined
their
function
adaptability
through
amplicon,
metagenome
metatranscriptome
sequencing.
found
that
activities
could
be
affected
potential
competition
inhibition
among
microbial
metabolic
pathways.
High-diversity
communities
generally
increased
abundance
expression
functional
genes.
Further
enrichment
experiments
showed
led
development
simplified
but
more
stable
communities,
characterized
by
similar
evolutionary
patterns
tighter
interactions,
adaptability.
Notably,
sustained
provision
NH4+
NO2-
during
potentially
strengthen
interconnections
denitrification,
anaerobic
ammonium
oxidation
(anammox)
dissimilatory
nitrate
reduction
(DNRA)
processes.
Moreover,
identification
shared
traits
anammox
DNRA
implies
important
cooperative
associations
microorganisms.
Our
findings
highlight
affect
adaptive
key
taxa
involved
removal.
