Structural Characterization and Anti-Gouty Nephropathy Potential of Polysaccharides from Atractylodes chinensis

Molecules, Год журнала: 2025, Номер 30(4), С. 757 - 757

Опубликована: Фев. 7, 2025

Polysaccharides derived from Atractylodes chinensis (DC.) Koidz. (ACP), a traditional Chinese medicine, were extracted and analyzed for their structural characteristics anti-gouty nephropathy (GN) activity. Sprague-Dawley (SD) rats divided into six groups: control, model, positive three treatment groups (ACP-60-L, ACP-60-M, ACP-60-H). Treatment significantly reduced inflammatory responses renal damage, as evidenced by decreased levels of uric acid (UA), creatinine (Cr), blood urea nitrogen (BUN), alongside modulation NOD-like receptor protein 3 (NLRP3) expression in tissues. ACP-60 was fractionated polysaccharides, including ACP-60-A (Mw 9.18 kDa), ACP-60-B 58.21 ACP-60-C 109.01 kDa) using DEAE-52 cellulose column chromatography. Monosaccharide analysis revealed that predominantly comprised fructose (Fru) glucose (Glc), contained rhamnose (Rha), galactose (Gal), Fru, mannose (Man), included Man, Gal, Rha xylose (Xyl). In vitro studies HK-2 cells confirmed the anti-GN activity all fractions, with demonstrating highest efficacy. Structural elucidation identified its main glycosidic linkages →1)-β-Fruf-(2→ backbone α-Glcp-(1→ β-Fruf-(2→ branches. The underlying mechanism ACP-60-A’s is associated inhibition NLRP3 inflammasome signaling pathway, suppression downstream factor release, downregulation NLRP3, ASC, Caspase-1 expression. Further demonstrated superior attributable to lower molecular weight, specific monosaccharide composition, unique bond arrangement. shows potential increased efficacy through purification or modification, laying groundwork developing novel therapeutic agents GN.

Язык: Английский

Structural characterization and bioactivities of a glucomannan-based polysaccharide (AGP-1) from Arundina graminifolia

Carbohydrate Polymer Technologies and Applications, Год журнала: 2025, Номер 10, С. 100758 - 100758

Опубликована: Март 17, 2025

Язык: Английский

Structural characterization of two novel heteropolysaccharides from Catharanthus roseus and the evaluation of their immunological activities

Carbohydrate Polymers, Год журнала: 2024, Номер 348, С. 122896 - 122896

Опубликована: Окт. 22, 2024

Язык: Английский