Structural Characterization and Anti-Gouty Nephropathy Potential of Polysaccharides from Atractylodes chinensis

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 757 - 757

Published: Feb. 7, 2025

Polysaccharides derived from Atractylodes chinensis (DC.) Koidz. (ACP), a traditional Chinese medicine, were extracted and analyzed for their structural characteristics anti-gouty nephropathy (GN) activity. Sprague-Dawley (SD) rats divided into six groups: control, model, positive three treatment groups (ACP-60-L, ACP-60-M, ACP-60-H). Treatment significantly reduced inflammatory responses renal damage, as evidenced by decreased levels of uric acid (UA), creatinine (Cr), blood urea nitrogen (BUN), alongside modulation NOD-like receptor protein 3 (NLRP3) expression in tissues. ACP-60 was fractionated polysaccharides, including ACP-60-A (Mw 9.18 kDa), ACP-60-B 58.21 ACP-60-C 109.01 kDa) using DEAE-52 cellulose column chromatography. Monosaccharide analysis revealed that predominantly comprised fructose (Fru) glucose (Glc), contained rhamnose (Rha), galactose (Gal), Fru, mannose (Man), included Man, Gal, Rha xylose (Xyl). In vitro studies HK-2 cells confirmed the anti-GN activity all fractions, with demonstrating highest efficacy. Structural elucidation identified its main glycosidic linkages →1)-β-Fruf-(2→ backbone α-Glcp-(1→ β-Fruf-(2→ branches. The underlying mechanism ACP-60-A’s is associated inhibition NLRP3 inflammasome signaling pathway, suppression downstream factor release, downregulation NLRP3, ASC, Caspase-1 expression. Further demonstrated superior attributable to lower molecular weight, specific monosaccharide composition, unique bond arrangement. shows potential increased efficacy through purification or modification, laying groundwork developing novel therapeutic agents GN.

Language: Английский

Structural characterization and bioactivities of a glucomannan-based polysaccharide (AGP-1) from Arundina graminifolia

Carbohydrate Polymer Technologies and Applications, Journal Year: 2025, Volume and Issue: 10, P. 100758 - 100758

Published: March 17, 2025

Language: Английский

Structural characterization of two novel heteropolysaccharides from Catharanthus roseus and the evaluation of their immunological activities

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 348, P. 122896 - 122896

Published: Oct. 22, 2024

Language: Английский