High-throughput screening for optimizing adoptive T cell therapies DOI Creative Commons
Yuchen Zhang,

Qinglong Xu,

Zhifei Gao

и другие.

Experimental Hematology and Oncology, Год журнала: 2024, Номер 13(1)

Опубликована: Ноя. 13, 2024

Abstract Adoptive T cell therapy is a pivotal strategy in cancer immunotherapy, demonstrating potent clinical efficacy. However, its limited durability often results primary resistance. High-throughput screening technologies, which include both genetic and non-genetic approaches, facilitate the optimization of adoptive therapies by enabling selection biologically significant targets or substances from extensive libraries. In this review, we examine advancements high-throughput technologies their applications therapies. We highlight use for cells, tumor other promising combination strategies, elucidate role identifying small molecules targeted delivery systems relevant to therapies, providing guidance future research applications.

Язык: Английский

Enhancing Vaccine Efficacy and Stability: A Review of the Utilization of Nanoparticles in mRNA Vaccines DOI Creative Commons
Nargish Parvin, Sang Woo Joo, Tapas Kumar Mandal

и другие.

Biomolecules, Год журнала: 2024, Номер 14(8), С. 1036 - 1036

Опубликована: Авг. 20, 2024

The development of vaccines has entered a new era with the advent nanotechnology, particularly through utilization nanoparticles. This review focuses on role nanoparticles in enhancing efficacy and stability mRNA vaccines. Nanoparticles, owing to their unique properties such as high surface area, tunable size, ability be functionalized, have emerged powerful tools vaccine development. Specifically, lipid (LNPs) revolutionized delivery by protecting fragile molecules facilitating efficient uptake cells. discusses various types employed formulations, including lipid-based, polymer-based, inorganic nanoparticles, highlighting advantages limitations. Moreover, it explores mechanisms which improve immune responses, enhanced antigen presentation prolonged release mRNA. also addresses challenges future directions nanoparticle-based development, emphasizing need for further research optimize formulations broader applications. By providing an in-depth analysis current advancements potential vaccines, this aims shed light critical combating infectious diseases improving public health outcomes.

Язык: Английский

Процитировано

5

Nonviral targeted mRNA delivery: principles, progresses, and challenges DOI Creative Commons
Xi He, Guohong Li, Li-Hsin Huang

и другие.

MedComm, Год журнала: 2025, Номер 6(1)

Опубликована: Янв. 1, 2025

Abstract Messenger RNA (mRNA) therapeutics have garnered considerable attention due to their remarkable efficacy in the treatment of various diseases. The COVID‐19 mRNA vaccine and RSV been approved on market. Due inherent nuclease‐instability negative charge mRNA, delivery systems are developed protect from degradation facilitate its crossing cell membrane express functional proteins or peptides cytoplasm. However, deficiency transfection efficiency targeted biological distribution still major challenges for systems. In this review, we first described physiological barriers process then discussed design approach recent advances with an emphasis tissue/cell‐targeted abilities. Finally, pointed out existing future directions deep insights into efficient We believe that a high‐precision system can greatly improve therapeutic effects bio‐safety accelerate clinical transformations. This review may provide new direction serve as useful guide researchers who looking suitable system.

Язык: Английский

Процитировано

0

Optimizing mRNA delivery: A microfluidic exploration of DOTMA vs. DOTAP lipid nanoparticles for GFP expression on human PBMCs and THP-1 cell line DOI Creative Commons
Erwin Pavel Lamparelli, Elena Ciaglia, Maria Camilla Ciardulli

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер 672, С. 125324 - 125324

Опубликована: Фев. 7, 2025

Язык: Английский

Процитировано

0

High-throughput screening for optimizing adoptive T cell therapies DOI Creative Commons
Yuchen Zhang,

Qinglong Xu,

Zhifei Gao

и другие.

Experimental Hematology and Oncology, Год журнала: 2024, Номер 13(1)

Опубликована: Ноя. 13, 2024

Abstract Adoptive T cell therapy is a pivotal strategy in cancer immunotherapy, demonstrating potent clinical efficacy. However, its limited durability often results primary resistance. High-throughput screening technologies, which include both genetic and non-genetic approaches, facilitate the optimization of adoptive therapies by enabling selection biologically significant targets or substances from extensive libraries. In this review, we examine advancements high-throughput technologies their applications therapies. We highlight use for cells, tumor other promising combination strategies, elucidate role identifying small molecules targeted delivery systems relevant to therapies, providing guidance future research applications.

Язык: Английский

Процитировано

1