Unleashing the Healing Power: 3D Bioprinting Mimics Hypoxia to Supercharge Mesenchymal Stem Cells
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 2, 2025
Mesenchymal
stem
cells
(MSCs)
play
a
critical
role
in
cell
therapy
due
to
their
tissue-mimicking
abilities.
However,
conventional
2D
culture
conditions
often
lead
the
loss
of
native
hypoxic
niche,
potentially
limiting
therapeutic
efficacy.
3D
bioprinting
offers
method
recreate
intricate
biological
environments
by
integrating
with
extracellular
matrices.
Therefore,
it
is
essential
adapt
printing
techniques
accurately
replicate
MSCs'
ecological
facilitating
integration
technology
into
clinical
applications.
In
this
study,
we
optimized
capabilities
using
performed
cellular
aggregates
(PCA)
method.
We
observed
that
printed
matrix
creates
microenvironment,
resulting
significant
increase
level
production
several
paracrine
signaling
molecules
and
immunomodulatory
factors
MSCs.
Furthermore,
MSCs
exhibited
enhanced
stemness
proliferative
capacity
early
stages
culture.
RNA-seq
analysis
revealed
these
changes
behavior
were
associated
environment
created
during
procedure
By
optimizing
bioink
composition
parameters,
successfully
simulated
vivo
leading
notable
improvements
MSC
characteristics
capacity.
RNA
sequencing
further
confirmed
activation
hypoxia
pathways,
which
are
crucial
for
properties.
These
findings
offer
valuable
insights
leveraging
MSC-based
therapies
regenerative
medicine.
Язык: Английский
Commercially available bioinks and state-of-the-art lab-made formulations for bone tissue engineering: a comprehensive review
Materials Today Bio,
Год журнала:
2024,
Номер
29, С. 101341 - 101341
Опубликована: Ноя. 14, 2024
Bioprinting
and
bioinks
are
two
of
the
game
changers
in
bone
tissue
engineering.
This
review
presents
different
bioprinting
technologies
including
extrusion-based,
inkjet-based,
laser-assisted,
light-based,
hybrid
with
their
own
strengths
weaknesses.
will
aid
researchers
selection
assessment
bioink;
discussion
ranges
from
commercially
available
to
custom
lab-made
formulations
mainly
based
on
natural
polymers,
such
as
agarose,
alginate,
gelatin,
collagen,
chitosan,
designed
for
The
is
centered
technological
advancements
increasing
clinical
demand
within
rapidly
growing
market.
From
this
point
view,
4D,
5D,
6D
printing
promise
a
future
where
unprecedented
levels
innovation
be
involved
fabrication
processes
leading
more
dynamic
multifunctionalities
bioprinted
constructs.
Further
advances
technology,
methods
covered,
meet
personalized
medicine
goals
while
advancing
patient
outcomes
tissues
engineering
applications.
Язык: Английский
Photocrosslinkable Biomaterials for 3D Bioprinting: Mechanisms, Recent Advances, and Future Prospects
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12567 - 12567
Опубликована: Ноя. 22, 2024
Constructing
scaffolds
with
the
desired
structures
and
functions
is
one
of
main
goals
tissue
engineering.
Three-dimensional
(3D)
bioprinting
a
promising
technology
that
enables
personalized
fabrication
devices
regulated
biological
mechanical
characteristics
similar
to
natural
tissues/organs.
To
date,
3D
has
been
widely
explored
for
biomedical
applications
like
engineering,
drug
delivery,
screening,
in
vitro
disease
model
construction.
Among
different
bioinks,
photocrosslinkable
bioinks
have
emerged
as
powerful
choice
advanced
devices,
fast
crosslinking
speed,
high
resolution,
great
print
fidelity.
The
biomaterials
used
light-based
printing
play
pivotal
role
functional
constructs.
Herein,
this
review
outlines
general
approaches
related
biomaterials,
including
extrusion-based
printing,
inkjet
stereolithography
laser-assisted
printing.
Further,
mechanisms,
advantages,
limitations
photopolymerization
photoinitiators
are
discussed.
Next,
recent
advances
synthetic
highlighted.
Finally,
challenges
future
perspectives
envisaged.
Язык: Английский
Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101512 - 101512
Опубликована: Янв. 22, 2025
The
generation
of
organized
3D
tissue
constructs
that
combines
cells
and
photo-crosslinkable
biomaterials
has
been
demonstrated
using
a
variety
bioprinting
technologies.
These
technologies
have
inspired
the
application
for
"in
situ"
bioprinting,
resulting
on
hand-held
tools
called
"Biopens"
can
transfer
capabilities
directly
into
hands
surgeons.
Here,
we
developed
validated
biopen
ophthalmological
applications,
specifically
corneal
stromal
regeneration
photochemical
crosslinking
(CXL),
as
well
cell
and,
potentially,
wound
healing.
We
used
to
CXL,
but
also
fast
processes.
Cytotoxicity,
viability
immunofluorescence
experiments
were
performed
with
human
stroma
keratocytes
(HCK)
loaded
inside
proposed
bioink
compositions.
Photochemical
cross-linking
was
evaluate
functionality
closure
in
porcine
eyes.
A
full-thickness
penetrating
incision,
5
mm
length
parallel
limbus
perpendicular
surface,
made
enucleated
cornea.
mechanical
properties
cornea
are
imitated
by
tuning
(GelMA/PEGDA/PI)
composition
parameters,
which
envisage
potential
being
translated
clinical
environment
closure.
Язык: Английский
Development of an alginate-based bioink with enhanced hemostatic and antibacterial properties
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
302, С. 140549 - 140549
Опубликована: Янв. 31, 2025
Язык: Английский
Advances in Digital Light Processing (DLP) Bioprinting: A Review of Biomaterials and Its Applications, Innovations, Challenges, and Future Perspectives
Polymers,
Год журнала:
2025,
Номер
17(9), С. 1287 - 1287
Опубликована: Май 7, 2025
Digital
light
processing
(DLP)
technology
stands
out
as
a
groundbreaking
method
in
the
field
of
biomedical
engineering
that
enables
production
highly
precise
structures
using
photopolymerizable
materials.
Smart
materials
such
shape
memory
polymers,
hydrogels,
and
nanocomposites
are
used
ideal
for
personalized
medicine
applications
thanks
to
their
properties
superior
mechanical
strength,
biocompatibility,
sensitivity
environmental
stimuli
DLP
technology.
The
integration
these
with
functional
complex
structures,
especially
areas
bone
soft
tissue
engineering,
drug
delivery,
biosensor
production.
However,
limited
material
diversity,
scalability
problems
processes,
technical
difficulties
optimizing
bioprinting
parameters
among
main
obstacles
this
field.
This
study
systematically
examines
role
smart
biomaterials
DLP-based
processes.
It
addresses
innovative
regenerative
medicine.
also
comprehensively
evaluates
its
contributions
discusses
future
research
overcome
current
limitations.
Язык: Английский
Emerging Trends in Microfluidic Biomaterials: From Functional Design to Applications
Lin Jiaqi,
Lijuan Cui,
Xiaokun Shi
и другие.
Journal of Functional Biomaterials,
Год журнала:
2025,
Номер
16(5), С. 166 - 166
Опубликована: Май 8, 2025
The
rapid
development
of
microfluidics
has
driven
innovations
in
material
engineering,
particularly
through
its
ability
to
precisely
manipulate
fluids
and
cells
at
microscopic
scales.
Microfluidic
biomaterials,
a
cutting-edge
interdisciplinary
field
integrating
microfluidic
technology
with
biomaterials
science,
are
revolutionizing
biomedical
research.
This
review
focuses
on
the
functional
design
fabrication
organ-on-a-chip
(OoAC)
platforms
via
3D
bioprinting,
explores
applications
drug
delivery,
cell
culture,
tissue
evaluates
potential
systems
advancing
personalized
healthcare.
We
systematically
analyze
evolution
materials—from
silicon
glass
polymers
paper—and
highlight
advantages
bioprinting
over
traditional
methods.
Currently,
despite
significant
advances
medicine,
challenges
scalability,
stability,
clinical
translation
remain.
future
will
depend
combining
dynamic
design,
developing
hybrid
strategies
that
combine
molds
bio-printed
structures,
using
artificial
intelligence
monitor
delivery
or
response
real
time.
believe
collaborations
between
materials
micromachining,
medicine
accelerate
into
therapies
high-throughput
screening
tools.
Язык: Английский
Technique of 3D printing for scaffolding in tissue engineering of bones: Opportunities and challenges
Materials Today Communications,
Год журнала:
2024,
Номер
42, С. 111249 - 111249
Опубликована: Дек. 9, 2024
Язык: Английский
3D bioprinting of the airways and lungs for applications in tissue engineering and in vitro models
Journal of Tissue Engineering,
Год журнала:
2024,
Номер
15
Опубликована: Янв. 1, 2024
Tissue
engineering
and
in
vitro
modeling
of
the
airways
lungs
respiratory
system
are
substantial
research
clinical
importance.
In
airway
lung
models
aim
to
improve
treatment
options
for
repair
advance
pathophysiological
research.
The
construction
biomimetic
native
with
tissue-specific
biological,
mechanical,
configurable
features
remains
challenging.
Bioprinting,
an
emerging
3D
printing
technology,
is
promising
development
airway,
lung,
disease
models,
allowing
incorporation
cells
biologically
active
molecules
into
printed
constructs
a
precise
reproducible
manner
recreate
airways,
architecture,
microenvironment.
Herein,
we
present
review
bioprinting
applications
tissue
modeling.
key
characteristics
interstitium,
alveoli
described.
bioinks
recently
used
summarized.
Furthermore,
propose
bioink
categorization
based
on
structural
airways.
Finally,
challenges
opportunities
biofabrication
discussed.
Язык: Английский