3D bioprinting of the airways and lungs for applications in tissue engineering and in vitro models DOI Creative Commons
Yanning Zhang, Yujian Liu, Chen Shu

et al.

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

Tissue engineering and in vitro modeling of the airways lungs respiratory system are substantial research clinical importance. In airway lung models aim to improve treatment options for repair advance pathophysiological research. The construction biomimetic native with tissue-specific biological, mechanical, configurable features remains challenging. Bioprinting, an emerging 3D printing technology, is promising development airway, lung, disease models, allowing incorporation cells biologically active molecules into printed constructs a precise reproducible manner recreate airways, architecture, microenvironment. Herein, we present review bioprinting applications tissue modeling. key characteristics interstitium, alveoli described. bioinks recently used summarized. Furthermore, propose bioink categorization based on structural airways. Finally, challenges opportunities biofabrication discussed.

Language: Английский

Commercially available bioinks and state-of-the-art lab-made formulations for bone tissue engineering: a comprehensive review DOI Creative Commons
Elena Alina Chiticaru, Mariana Ioniţă

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101341 - 101341

Published: Nov. 14, 2024

Bioprinting and bioinks are two of the game changers in bone tissue engineering. This review presents different bioprinting technologies including extrusion-based, inkjet-based, laser-assisted, light-based, hybrid with their own strengths weaknesses. will aid researchers selection assessment bioink; discussion ranges from commercially available to custom lab-made formulations mainly based on natural polymers, such as agarose, alginate, gelatin, collagen, chitosan, designed for The is centered technological advancements increasing clinical demand within rapidly growing market. From this point view, 4D, 5D, 6D printing promise a future where unprecedented levels innovation be involved fabrication processes leading more dynamic multifunctionalities bioprinted constructs. Further advances technology, methods covered, meet personalized medicine goals while advancing patient outcomes tissues engineering applications.

Language: Английский

Citations

5
Photochemical corneal cross-linking: Evaluating the potential of a hand-held biopen DOI Creative Commons
Nadina Usseglio, Julia López de Andrés, Juan Antonio Marchal

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101512 - 101512

Published: Jan. 22, 2025

The generation of organized 3D tissue constructs that combines cells and photo-crosslinkable biomaterials has been demonstrated using a variety bioprinting technologies. These technologies have inspired the application for "in situ" bioprinting, resulting on hand-held tools called "Biopens" can transfer capabilities directly into hands surgeons. Here, we developed validated biopen ophthalmological applications, specifically corneal stromal regeneration photochemical crosslinking (CXL), as well cell and, potentially, wound healing. We used to CXL, but also fast processes. Cytotoxicity, viability immunofluorescence experiments were performed with human stroma keratocytes (HCK) loaded inside proposed bioink compositions. Photochemical cross-linking was evaluate functionality closure in porcine eyes. A full-thickness penetrating incision, 5 mm length parallel limbus perpendicular surface, made enucleated cornea. mechanical properties cornea are imitated by tuning (GelMA/PEGDA/PI) composition parameters, which envisage potential being translated clinical environment closure.

Language: Английский

Citations

0
Development of an alginate-based bioink with enhanced hemostatic and antibacterial properties DOI
Mengde Zhang,

Linhao Hou,

Wei Song

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 302, P. 140549 - 140549

Published: Jan. 31, 2025

Language: Английский

Citations

0
Unleashing the Healing Power: 3D Bioprinting Mimics Hypoxia to Supercharge Mesenchymal Stem Cells DOI
Yuyan Huang, Liting Liang, Yi Kong

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 2, 2025

Mesenchymal stem cells (MSCs) play a critical role in cell therapy due to their tissue-mimicking abilities. However, conventional 2D culture conditions often lead the loss of native hypoxic niche, potentially limiting therapeutic efficacy. 3D bioprinting offers method recreate intricate biological environments by integrating with extracellular matrices. Therefore, it is essential adapt printing techniques accurately replicate MSCs' ecological facilitating integration technology into clinical applications. In this study, we optimized capabilities using performed cellular aggregates (PCA) method. We observed that printed matrix creates microenvironment, resulting significant increase level production several paracrine signaling molecules and immunomodulatory factors MSCs. Furthermore, MSCs exhibited enhanced stemness proliferative capacity early stages culture. RNA-seq analysis revealed these changes behavior were associated environment created during procedure By optimizing bioink composition parameters, successfully simulated vivo leading notable improvements MSC characteristics capacity. RNA sequencing further confirmed activation hypoxia pathways, which are crucial for properties. These findings offer valuable insights leveraging MSC-based therapies regenerative medicine.

Language: Английский

Citations

0
Photocrosslinkable Biomaterials for 3D Bioprinting: Mechanisms, Recent Advances, and Future Prospects DOI Open Access

Yushang Lai,

Xiong Xiao, Ziwei Huang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12567 - 12567

Published: Nov. 22, 2024

Constructing scaffolds with the desired structures and functions is one of main goals tissue engineering. Three-dimensional (3D) bioprinting a promising technology that enables personalized fabrication devices regulated biological mechanical characteristics similar to natural tissues/organs. To date, 3D has been widely explored for biomedical applications like engineering, drug delivery, screening, in vitro disease model construction. Among different bioinks, photocrosslinkable bioinks have emerged as powerful choice advanced devices, fast crosslinking speed, high resolution, great print fidelity. The biomaterials used light-based printing play pivotal role functional constructs. Herein, this review outlines general approaches related biomaterials, including extrusion-based printing, inkjet stereolithography laser-assisted printing. Further, mechanisms, advantages, limitations photopolymerization photoinitiators are discussed. Next, recent advances synthetic highlighted. Finally, challenges future perspectives envisaged.

Language: Английский

Citations

3
Emerging Trends in Microfluidic Biomaterials: From Functional Design to Applications DOI Creative Commons

Lin Jiaqi,

Lijuan Cui,

Xiaokun Shi

et al.

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(5), P. 166 - 166

Published: May 8, 2025

The rapid development of microfluidics has driven innovations in material engineering, particularly through its ability to precisely manipulate fluids and cells at microscopic scales. Microfluidic biomaterials, a cutting-edge interdisciplinary field integrating microfluidic technology with biomaterials science, are revolutionizing biomedical research. This review focuses on the functional design fabrication organ-on-a-chip (OoAC) platforms via 3D bioprinting, explores applications drug delivery, cell culture, tissue evaluates potential systems advancing personalized healthcare. We systematically analyze evolution materials—from silicon glass polymers paper—and highlight advantages bioprinting over traditional methods. Currently, despite significant advances medicine, challenges scalability, stability, clinical translation remain. future will depend combining dynamic design, developing hybrid strategies that combine molds bio-printed structures, using artificial intelligence monitor delivery or response real time. believe collaborations between materials micromachining, medicine accelerate into therapies high-throughput screening tools.

Language: Английский

Citations

0
Technique of 3D printing for scaffolding in tissue engineering of bones: Opportunities and challenges DOI
Ramankit Jaiswal, Rita Wadetwar

Materials Today Communications, Journal Year: 2024, Volume and Issue: 42, P. 111249 - 111249

Published: Dec. 9, 2024

Language: Английский

Citations

1
3D bioprinting of the airways and lungs for applications in tissue engineering and in vitro models DOI Creative Commons
Yanning Zhang, Yujian Liu, Chen Shu

et al.

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

Tissue engineering and in vitro modeling of the airways lungs respiratory system are substantial research clinical importance. In airway lung models aim to improve treatment options for repair advance pathophysiological research. The construction biomimetic native with tissue-specific biological, mechanical, configurable features remains challenging. Bioprinting, an emerging 3D printing technology, is promising development airway, lung, disease models, allowing incorporation cells biologically active molecules into printed constructs a precise reproducible manner recreate airways, architecture, microenvironment. Herein, we present review bioprinting applications tissue modeling. key characteristics interstitium, alveoli described. bioinks recently used summarized. Furthermore, propose bioink categorization based on structural airways. Finally, challenges opportunities biofabrication discussed.

Language: Английский

Citations

0