The development of highly dense highly protected surfactant ionizable lipid RNA loaded nanoparticles DOI Creative Commons

Ramon González-Rioja,

Vivian A. Salazar, Neus G. Bastús

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Фев. 27, 2023

The long quest for efficient drug administration has been looking a universal carrier that can precisely transport traditional drugs, new genomic and proteic therapeutic agents. Today, researchers have found conditions to overcome the two main delivery dilemmas. On one side, versatility of vehicle efficiently load, protect then release it at target place. other hand, questions related degree PEGylation which are needed avoid nanoparticle (NP) aggregation opsonization while preventing cellular uptake. development different kinds lipidic vehicles particles resulted in ionizable lipid nanoparticles (iLNPs), most typical problems. Proof their success is late approval massive as prophylactic vaccine SARS-CoV-2. These ILNPs built by electrostatic surfactants, agent, lipids self-segregate from an aqueous solution, forming stabilized with polymers, such PEG. previous limitations low loading high toxicity, likely thanks charge working pH reduced size, entry into cells via endocytosis rather than membrane perforation or fusion, always associated higher toxicity. We herein revise primary features, synthetic methods prepare characterize them, pharmacokinetic (administration, distribution, metabolization excretion) aspects, biodistribution fate. Owing advantages, iLNPs potential systems improve management various diseases widely available clinical use.

Язык: Английский

Nanotechnology in the COVID-19 era: Carbon-based nanomaterials as a promising solution DOI Creative Commons
Massimiliano Papi, Marco De Spirito, Valentina Palmieri

и другие.

Carbon, Год журнала: 2023, Номер 210, С. 118058 - 118058

Опубликована: Апрель 29, 2023

The Coronavirus Disease 2019 (COVID-19) pandemic has led to collaboration between nanotechnology scientists, industry stakeholders, and clinicians develop solutions for diagnostics, prevention, treatment of Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) infections. Nanomaterials, including carbon-based materials (CBM) such as graphene carbon nanotubes, have been studied their potential in viral research. CBM unique effects on microorganisms, immune interaction, sensitivity diagnostics made them a promising subject SARS-CoV-2 This review discusses the interaction with applicability, physical chemical properties, known interactions components, proposed treatment, uses.

Язык: Английский

Процитировано

12
Clinical and Molecular Characterization of a Rare Case of BNT162b2 mRNA COVID-19 Vaccine-Associated Myositis DOI Creative Commons
Eli Magen, Sumit Mukherjee, Mahua Bhattacharya

и другие.

Vaccines, Год журнала: 2022, Номер 10(7), С. 1135 - 1135

Опубликована: Июль 16, 2022

Initial clinical trials and surveillance data have shown that the most commonly administered BNT162b2 COVID-19 mRNA vaccine is effective safe. However, several cases of vaccine-induced mild to moderate adverse events were recently reported. Here, we report a rare case myositis after injection first dose into left deltoid muscle 34-year-old, previously healthy woman who presented progressive proximal weakness, dysphagia, dyspnea with respiratory failure. One month vaccination, expression was detected in tissue biopsy right quadriceps muscles. We propose this as example myositis. This study comprehensively characterizes molecular features vaccine-associated which patient severely affected.

Язык: Английский

Процитировано

18
Peptide delivery of a multivalent mRNA SARS-CoV-2 vaccine DOI Creative Commons

Cian M. McCrudden,

Lindsey Bennie, Philip Chambers

и другие.

Journal of Controlled Release, Год журнала: 2023, Номер 362, С. 536 - 547

Опубликована: Сен. 9, 2023

Lipid nanoparticles (LNP) have been instrumental in the success of mRNA vaccines and opened up field to a new wave therapeutics. However, what is ahead beyond LNP? The approach herein used nanoparticle containing blend Spike, Membrane Envelope antigens complexed for first time with RALA peptide (RALA-SME). physicochemical characteristics functionality RALA-SME were assessed. With >99% encapsulation, was administered via intradermal injection vivo, all three antigen-specific IgG antibodies highly significant. IgG2a:IgG1 ratio >1.2, indicating robust TH1 response, this further confirmed T-Cell response mice. A complete safety panel markers from mice within normal range, supported by data hamsters. Vaccination Syrian Golden hamsters derivatives produced functional capable neutralising SARS-CoV-2 both Wuhan-Hu-1 Omicron BA.1 lineages after two doses. Antibody levels increased over study period provided protection disease-specific weight loss, inhibition viral migration down respiratory tract. This technology enables flexibility interchange add as required, which essential next generation adaptable vaccines.

Язык: Английский

Процитировано

11
The Transformative Potential of Lipid Nanoparticle–Protein Corona for Next-Generation Vaccines and Therapeutics DOI Creative Commons
Augusto Amici, Daniela Pozzi, Cristina Marchini

и другие.

Molecular Pharmaceutics, Год журнала: 2023, Номер 20(11), С. 5247 - 5253

Опубликована: Окт. 2, 2023

The integration of the lipid nanoparticle (LNP)-protein corona as a pioneering approach for development vaccines against present and future SARS-CoV-2 variants concern marks significant shift in field. This concept holds great promise, offering universal platform that can be adaptable to combat pandemics caused by unknown viruses. Understanding complex interactions among protein corona, LNPs, receptors is crucial harnessing its potential. knowledge will allow optimal vaccine formulations improve their effectiveness. Safety assessments are essential ensure suitability human use, compliance with regulatory standards, rigorous quality control manufacturing. transformative workflow requires collaborative efforts, expanding our foundational translating advancements from laboratory clinical reality. LNP-protein represents paradigmatic far-reaching implications. Its principles insights leveraged beyond specific applications SARS-CoV-2, enabling addressing viral threats, cancer, genetic diseases.

Язык: Английский

Процитировано

11
The development of highly dense highly protected surfactant ionizable lipid RNA loaded nanoparticles DOI Creative Commons

Ramon González-Rioja,

Vivian A. Salazar, Neus G. Bastús

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Фев. 27, 2023

The long quest for efficient drug administration has been looking a universal carrier that can precisely transport traditional drugs, new genomic and proteic therapeutic agents. Today, researchers have found conditions to overcome the two main delivery dilemmas. On one side, versatility of vehicle efficiently load, protect then release it at target place. other hand, questions related degree PEGylation which are needed avoid nanoparticle (NP) aggregation opsonization while preventing cellular uptake. development different kinds lipidic vehicles particles resulted in ionizable lipid nanoparticles (iLNPs), most typical problems. Proof their success is late approval massive as prophylactic vaccine SARS-CoV-2. These ILNPs built by electrostatic surfactants, agent, lipids self-segregate from an aqueous solution, forming stabilized with polymers, such PEG. previous limitations low loading high toxicity, likely thanks charge working pH reduced size, entry into cells via endocytosis rather than membrane perforation or fusion, always associated higher toxicity. We herein revise primary features, synthetic methods prepare characterize them, pharmacokinetic (administration, distribution, metabolization excretion) aspects, biodistribution fate. Owing advantages, iLNPs potential systems improve management various diseases widely available clinical use.

Язык: Английский

Процитировано

9