International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12983 - 12983
Опубликована: Дек. 3, 2024
Nonmelanoma
skin
cancer
(NMSC)
presents
a
significant
challenge
to
global
healthcare
due
its
rising
incidence,
prompting
the
search
for
innovative
treatments
overcome
limitations
of
current
therapies.
Our
study
aims
explore
potential
effects
liquid
blood
concentrate
platelet-rich
fibrin
(PRF)
on
basal
cell
carcinoma
cells
(BCCs)
and
squamous
(SCCs)
in
order
obtain
results
that
may
lead
new
possible
adjunctive
therapies
managing
localized
cancers,
particularly
NMSC.
Basal
(BCC)
(SCC)
were
indirectly
treated
with
PRF
generated
via
different
relative
centrifugation
forces,
namely
high
low
RCF
PRF,
7
days.
PRF-treated
comparatively
analyzed
viability,
proliferation
cycle-
apoptosis-related
gene
expression.
Analysis
MTS
assay
revealed
decrease
viability
both
BCC
SCC
following
treatment
Ki-67
staining
showed
decreased
percentage
Ki-67-positive
after
2
days
compared
control
group.
The
downregulation
CCND1
expression
types
at
along
upregulation
p21
p53
demonstrated
effect
inhibiting
inducing
cycle
arrest,
especially
during
initial
phases
treatment.
Increased
caspase-8
caspase-9
was
observed,
indicating
activation
extrinsic
intrinsic
apoptotic
pathways
by
Although
exact
immunomodulatory
properties
require
further
investigation,
our
basic
vitro
studies
are
promising
might
provide
basis
future
investigations
as
an
therapy
IntechOpen eBooks,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 18, 2024
Skin
cancer
has
been
associated
with
excessive
sun
exposure
ever
since
the
nineteenth
century.
The
most
common
affected
skin
cells
upon
recurrent
to
include
squamous
cells,
basal
and
Merkel
cells.
Basal
cell
carcinoma
highest
prevalence
among
other
types
of
cancer,
but
rarely
metastasizes
is
highly
treatable.
Squamous
carcinoma,
if
left
untreated,
inclined
penetrate
epidermis,
invade
into
dermis,
metastasize
organs.
Melanoma,
however,
aggressive
least
survival
rates.
Cumulative
ultraviolet
radiation
mainly
from
results
in
DNA
damage,
oxidative
stress,
inflammatory
response,
gene
mutations,
all
leading
development
cancer.
Many
molecular
pathways
are
by
mutations
can
activate
oncogenes,
inactivate
tumor
suppressor
genes,
or
impair
repair
genes.
This
consequently
lead
increased
proliferation,
blood
vessel
growth,
invasion,
evasion
immune
ultimately,
metastasis.
Early
detection
vital
prevent
promotion
via
histopathological
techniques.
Treatment
options
surgical
removal
as
first-line
treatment
for
early
stages
therapy.
An
alternative
emerging
approach
metastasized
through
nanotechnology
that
operates
at
a
nanoscale
increasing
drug
delivery
distribution
body.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12983 - 12983
Опубликована: Дек. 3, 2024
Nonmelanoma
skin
cancer
(NMSC)
presents
a
significant
challenge
to
global
healthcare
due
its
rising
incidence,
prompting
the
search
for
innovative
treatments
overcome
limitations
of
current
therapies.
Our
study
aims
explore
potential
effects
liquid
blood
concentrate
platelet-rich
fibrin
(PRF)
on
basal
cell
carcinoma
cells
(BCCs)
and
squamous
(SCCs)
in
order
obtain
results
that
may
lead
new
possible
adjunctive
therapies
managing
localized
cancers,
particularly
NMSC.
Basal
(BCC)
(SCC)
were
indirectly
treated
with
PRF
generated
via
different
relative
centrifugation
forces,
namely
high
low
RCF
PRF,
7
days.
PRF-treated
comparatively
analyzed
viability,
proliferation
cycle-
apoptosis-related
gene
expression.
Analysis
MTS
assay
revealed
decrease
viability
both
BCC
SCC
following
treatment
Ki-67
staining
showed
decreased
percentage
Ki-67-positive
after
2
days
compared
control
group.
The
downregulation
CCND1
expression
types
at
along
upregulation
p21
p53
demonstrated
effect
inhibiting
inducing
cycle
arrest,
especially
during
initial
phases
treatment.
Increased
caspase-8
caspase-9
was
observed,
indicating
activation
extrinsic
intrinsic
apoptotic
pathways
by
Although
exact
immunomodulatory
properties
require
further
investigation,
our
basic
vitro
studies
are
promising
might
provide
basis
future
investigations
as
an
therapy
