Identification of a Circadian Output Circuit for Rest:Activity Rhythms in Drosophila

Cell, Год журнала: 2014, Номер 157(3), С. 689 - 701

Опубликована: Апрель 1, 2014

Язык: Английский

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A review of neurohormone GPCRs present in the fruitfly Drosophila melanogaster and the honey bee Apis mellifera

Progress in Neurobiology, Год журнала: 2006, Номер 80(1), С. 1 - 19

Опубликована: Сен. 1, 2006

Язык: Английский

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The Neuropeptide Pigment-Dispersing Factor Adjusts Period and Phase ofDrosophila's Clock

Journal of Neuroscience, Год журнала: 2009, Номер 29(8), С. 2597 - 2610

Опубликована: Фев. 25, 2009

The neuropeptide pigment-dispersing factor (PDF) is a key transmitter in the circadian clock of Drosophila melanogaster. PDF necessary for robust activity rhythms and thought to couple oscillations neurons. However, little known about action on individual Here, we combined period –luciferase reporter system with immunolabeling proteins wild-type Pdf 01 mutants dissect effects specific subgroups Additionally, levels were elevated higher than normal using neural mutants, correlation analysis locomotor protein staining served determine periods cells. We found that has multiple neurons: In some groups neurons, was required maintaining cells, others, synchronous cycling members. Other neurons cycled high amplitude absence PDF, but affected their intrinsic speed. Sometimes shortened sometimes lengthened period. Our observations indicate crucial adjusting amplitude, period, phase different players clock. Under natural conditions may be adapting Drosophila' s varying photoperiods. Indeed, show here are not able adapt long photoperiods manner.

Язык: Английский

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A genome-wide inventory of neurohormone GPCRs in the red flour beetle Tribolium castaneum

Frontiers in Neuroendocrinology, Год журнала: 2007, Номер 29(1), С. 142 - 165

Опубликована: Ноя. 26, 2007

Язык: Английский

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Circadian Remodeling of Neuronal Circuits Involved in Rhythmic Behavior

PLoS Biology, Год журнала: 2008, Номер 6(3), С. e69 - e69

Опубликована: Март 18, 2008

Clock output pathways are central to convey timing information from the circadian clock a diversity of physiological systems, ranging cell-autonomous processes behavior. While molecular mechanisms that generate and sustain rhythmicity at cellular level well understood, it is unclear how this further structured control specific behavioral outputs. Rhythmic release pigment dispersing factor (PDF) has been proposed propagate time day core pacemaker cells downstream targets underlying rhythmic locomotor activity. Indeed, such changes in PDF intensity represent only known mechanism through which circuit could communicate with its output. Here we describe novel phenomenon involving extensive remodeling axonal terminals circuit, display higher complexity during significantly lower nighttime, both under daily cycles constant conditions. In support nature, cycling lost bona fide clockless mutants. We propose clock-controlled structural plasticity as candidate contributing transmission cells.

Язык: Английский

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Development and morphology of the clock‐gene‐expressing lateral neurons of Drosophila melanogaster

The Journal of Comparative Neurology, Год журнала: 2006, Номер 500(1), С. 47 - 70

Опубликована: Ноя. 10, 2006

The clock-gene-expressing lateral neurons are essential for the locomotor activity rhythm of Drosophila melanogaster. Traditionally, these divided into three groups: dorsal (LN(d)), large ventral (l-LN(v)), and small (s-LN(v)), whereby latter group consists four that express neuropeptide pigment-dispersing factor (PDF) a fifth PDF-negative neuron. So far, only l-LN(v) PDF-positive s-LN(v) have been shown to project accessory medulla, neuropil contains circadian pacemaker center in several insects. We show here other also arborize predominantly forming postsynaptic sites. Both LN(d) anatomically well suited connect medullae. Whereas may receive ipsilateral photic input from Hofbauer-Buchner eyelet, invade mainly contralateral medulla thus side. differentiate during midmetamorphosis. They do so close proximity one another s-LN(v), suggesting cell groups derive common precursors.

Язык: Английский

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Setting the clock – by nature: Circadian rhythm in the fruitfly Drosophila melanogaster

FEBS Letters, Год журнала: 2011, Номер 585(10), С. 1435 - 1442

Опубликована: Фев. 25, 2011

Nowadays humans mainly rely on external, unnatural clocks such as of cell phones and alarm – driven by circuit boards electricity. Nevertheless, our body is under the control another timer firmly anchored in genes. This evolutionary very old biological clock drives most physiology behavior. The genes that internal are conserved among living beings. One organism shares this ancient mechanism with us fruitfly Drosophila melanogaster . Since it turned out an excellent model, no surprise its well intensely investigated. In following review we want to display overview current understanding Drosophila's circadian clock.

Язык: Английский

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Peptidergic clock neurons in Drosophila: Ion transport peptide and short neuropeptide F in subsets of dorsal and ventral lateral neurons

The Journal of Comparative Neurology, Год журнала: 2009, Номер 516(1), С. 59 - 73

Опубликована: Май 15, 2009

About 150 clock neurons are clustered in different groups the brain of Drosophila. Among these neurons, some pigment-dispersing factor (PDF)-positive and PDF-negative lateral (LNs) principal oscillators responsible for bouts activity morning evening, respectively. The full complement neurotransmitters evening is not known. By using a screen candidate neuromediators we discovered ion transport peptide (ITP) short neuropeptide F (sNPF) as novel neuropeptides subpopulations dorsal (LN(d)s) ventral (s-LN(v)s) LNs. six LN(d)s, ITP was found one that coexpresses long (NPF) cryptochrome. We detected sNPF two LN(d)s also express cryptochrome; cells distinct from three expressing NPF. Thus, have identified five LN(d)s. cryptochrome, with either or sNPF, only ones additional projections to accessory medulla. s-LN(v)s adult brain, fifth neuron devoid PDF four PDF. choline acetyltransferase (Cha) Gal4, Cha expression producing s-LN(v). In larval PDF-producing coexpress sNPF. Our findings emphasize heterogeneous both anatomically respect content neuropeptides, other markers suggest diverse functions neurons.

Язык: Английский

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Neuropeptide Receptor Transcriptome Reveals Unidentified Neuroendocrine Pathways

PLoS ONE, Год журнала: 2008, Номер 3(8), С. e3048 - e3048

Опубликована: Авг. 22, 2008

Neuropeptides are an important class of molecules involved in diverse aspects metazoan development and homeostasis. Insects ideal model systems to investigate neuropeptide functions, the major focus insect research last decade has been on identification their receptors. Despite these vigorous efforts, receptors for some key neuropeptides such as prothoracicotropic hormone, eclosion hormone allatotropin (AT), remain undefined. In this paper, we report comprehensive cloning G protein-coupled from silkworm, Bombyx mori, systematic analyses expression. Based expression patterns orphan receptors, identified long-sought receptor AT, which is thought stimulate juvenile biosynthesis corpora allata (CA). Surprisingly, however, AT was not highly expressed CA, but instead predominantly transcribed cardiaca (CC), organ adjacent CA. Indeed, by using a reverse-physiological approach, purified characterized novel allatoregulatory peptides produced receptor-expressing CC cells, may indirectly mediate activity All above findings confirm effectiveness analysis transcriptome, only characterizing also identifying players hidden mechanisms biological processes. This work illustrates how combinatorial approach employing bioinformatic, molecular, biochemical physiological methods can help solve recalcitrant problems research.

Язык: Английский

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The functional organisation of glia in the adult brain of Drosophila and other insects

Progress in Neurobiology, Год журнала: 2010, Номер 90(4), С. 471 - 497

Опубликована: Янв. 29, 2010

Язык: Английский

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