ACS Photonics, Год журнала: 2025, Номер unknown
Опубликована: Апрель 4, 2025
Язык: Английский
Cell, Год журнала: 2022, Номер 185(1), С. 9 - 41
Опубликована: Янв. 1, 2022
Язык: Английский
Процитировано
118
Nature Communications, Год журнала: 2021, Номер 12(1)
Опубликована: Ноя. 17, 2021
Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, offers subcellular resolution over a large ~25 mm
Язык: Английский
Процитировано
108
Molecular Psychiatry, Год журнала: 2023, Номер 28(8), С. 3194 - 3206
Опубликована: Авг. 1, 2023
Social behaviors, how individuals act cooperatively and competitively with conspecifics, are widely seen across species. Rodents display various social many different behavioral paradigms have been used for investigating their neural circuit bases. behavior is highly vulnerable to brain network dysfunction caused by neurological neuropsychiatric conditions such as autism spectrum disorders (ASDs). Studying mouse models of ASD provides a promising avenue toward elucidating mechanisms abnormal potential therapeutic targets treatment. In this review, we outline recent progress key findings on underlying behavior, particular emphasis rodent studies that monitor manipulate the activity specific circuits using modern systems neuroscience approaches. mediated distributed brain-wide among major cortical (e.g., medial prefrontal cortex (mPFC), anterior cingulate cortex, insular (IC)) subcortical nucleus accumbens, basolateral amygdala (BLA), ventral tegmental area) structures, influenced multiple neuromodulatory oxytocin, dopamine, serotonin). We particularly draw special attention IC unique area mediates multisensory integration, encoding ongoing interaction, decision-making, emotion, empathy. Additionally, synthesis demonstrates dysfunctions in mPFC-BLA circuitry neuromodulation prominent. Pharmacological rescues local or systemic oral) administration drugs provided valuable clues developing new agents ASD. Future efforts technological advances will push forward next frontiers field, elucidation inter-brain dynamics during real virtual interactions, establishment circuit-based therapy affecting functions.
Язык: Английский
Процитировано
58
Opto-Electronic Advances, Год журнала: 2024, Номер 7(6), С. 230212 - 230212
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
23
Materials Today, Год журнала: 2023, Номер 62, С. 251 - 270
Опубликована: Янв. 1, 2023
Язык: Английский
Процитировано
31
Nature Methods, Год журнала: 2024, Номер 21(5), С. 897 - 907
Опубликована: Март 21, 2024
cAMP is a universal second messenger regulated by various upstream pathways including Ca
Язык: Английский
Процитировано
15
Nature Photonics, Год журнала: 2024, Номер 18(7), С. 721 - 730
Опубликована: Апрель 17, 2024
Abstract Benefitting from the advantages of high imaging throughput and low cost, wide-field microscopy has become indispensable in biomedical studies. However, it remains challenging to record biodynamics with a large field view spatiotemporal resolution due limited space–bandwidth product. Here we propose random-access (RA-WiFi) mesoscopy for vivo over 163.84 mm 2 area spatial ~2.18 μm. We extend beyond nominal value objective by enlarging object distance, which leads lower angle, followed correction optical aberrations. also implement scanning structured illumination, enables optical-sectioning capability contrast. The multi-plane makes technique suitable curved-surface samples. demonstrate RA-WiFi multi-modal imaging, including bright-field, dark-field multi-colour fluorescence imaging. Specifically, apply calcium cortex-wide neural network activities awake mice vivo, under both physiological pathological conditions. show its unique three-dimensional random access irregular regions interest via biodynamic mouse spinal cords vivo. As compact, low-cost mesoscope capability, will enable broad applications study biological systems.
Язык: Английский
Процитировано
11
Light Science & Applications, Год журнала: 2025, Номер 14(1)
Опубликована: Янв. 1, 2025
Abstract In recent advancements in life sciences, optical microscopy has played a crucial role acquiring high-quality three-dimensional structural and functional information. However, the quality of 3D images is often compromised due to intense scattering effect biological tissues, compounded by several issues such as limited spatiotemporal resolution, low signal-to-noise ratio, inadequate depth penetration, high phototoxicity. Although various sectioning techniques have been developed address these challenges, each method adheres distinct imaging principles for specific applications. As result, effective selection suitable across diverse scenarios become yet challenging. This paper comprehensively overviews existing guidance under different scenarios. Specifically, we categorize microscope design based on spatial relationship between illumination detection axis, i.e., on-axis off-axis. classification provides unique perspective compare implementation performances approaches. Lastly, integrate selected methods custom-built off-axis system present future development techniques.
Язык: Английский
Процитировано
1
Neurophotonics, Год журнала: 2022, Номер 9(S1)
Опубликована: Апрель 27, 2022
Neurophotonics was launched in 2014 coinciding with the launch of BRAIN Initiative focused on development technologies for advancement neuroscience. For last seven years, Neurophotonics' agenda has been well aligned this focus neurotechnologies featuring new optical methods and tools applicable to brain studies. While 2.0 is pivoting towards applications these novel quest understand brain, article we review an extensive diverse toolkit explore function that have emerged from related large-scale efforts measurement manipulation structure function. Here, neurophotonic mostly animal A companion article, scheduled appear later year, will cover diffuse imaging noninvasive human each domain, outline current state-of-the-art respective technologies, identify areas where innovation needed provide outlook future directions.
Язык: Английский
Процитировано
33
Biology, Год журнала: 2022, Номер 11(11), С. 1601 - 1601
Опубликована: Ноя. 1, 2022
A central tenet of neuroscience is that sensory, motor, and cognitive behaviors are generated by the communications interactions among neurons, distributed within across anatomically functionally distinct brain regions. Therefore, to decipher how plans, learns, executes requires characterizing neuronal activity at multiple spatial temporal scales. This includes simultaneously recording dynamics mesoscale level understand regions during different behavioral states. Wide-field Ca2+ imaging, which uses single photon excitation improved genetically encoded indicators, allows for simultaneous recordings large areas proving be a powerful tool study mesoscopic scale in behaving animals. review details techniques used wide-field imaging various approaches employed analyses rich neuronal-behavioral data sets obtained. Also discussed providing novel insights into both normal altered neural processing disease. Finally, we examine limitations approach new developments bringing capabilities this important technique investigating large-scale dynamics.
Язык: Английский
Процитировано
30