Neurophotonics, Год журнала: 2024, Номер 11(S1)
Опубликована: Июнь 5, 2024
An emerging trend at the forefront of optical neural interfaces leverages properties photonic nanostructures to modulate light delivery and collection patterns in deep brain regions. This perspective article surveys early works that have spearheaded this promising strategy, discusses its promise towards establishment a class augmented nano-neurophotonic probes.
Язык: Английский
Progress in Neurobiology, Год журнала: 2024, Номер 234, С. 102571 - 102571
Опубликована: Янв. 22, 2024
Язык: Английский
Процитировано
9
Annual Review of Plant Biology, Год журнала: 2023, Номер 74(1), С. 313 - 339
Опубликована: Май 22, 2023
Optogenetics is a technique employing natural or genetically engineered photoreceptors in transgene organisms to manipulate biological activities with light. Light can be turned on off, and adjusting its intensity duration allows optogenetic fine-tuning of cellular processes noninvasive spatiotemporally resolved manner. Since the introduction Channelrhodopsin-2 phytochrome-based switches nearly 20 years ago, tools have been applied variety model enormous success, but rarely plants. For long time, dependence plant growth light absence retinal, rhodopsin chromophore, prevented establishment optogenetics until recent progress overcame these difficulties. We summarize results work field control motion via green light–gated ion channels present successful applications light-control gene expression single combined photoswitches Furthermore, we highlight technical requirements options for future research.
Язык: Английский
Процитировано
19
Frontiers in Neuroscience, Год журнала: 2024, Номер 18
Опубликована: Май 3, 2024
The human brain, with its vast network of billions neurons and trillions synapses (connections) between diverse cell types, remains one the greatest mysteries in science medicine. Despite extensive research, an understanding underlying mechanisms that drive normal behaviors response to disease states is still limited. Advancement Neuroscience field development therapeutics for related pathologies requires innovative technologies can provide a dynamic systematic interactions neural circuits. In this work, we up-to-date overview evolution neurophotonic approaches last 10 years through multi-source, literature analysis. From initial corpus 243 papers retrieved from Scopus, PubMed WoS databases, have followed PRISMA approach select 56 area. Following full-text evaluation these scientific articles, six main areas applied research were identified discussed: (1) Advanced optogenetics, (2) Multimodal interfaces, (3) Innovative therapeutics, (4) Imaging devices probes, (5) Remote operations, (6) Microfluidic platforms. For each area, selected are discussed according photonic principles applied, neuroscience application evaluated more indicative results efficiency potential. This detailed analysis by outlook challenges tackled over Neurophotonics field, as well technological advances regarding specificity, light delivery, multimodality, imaging, materials system designs. We conclude discussion considerable future innovation translation Neurophotonics, delivery within brain physical constraints data management strategies.
Язык: Английский
Процитировано
7
Neurophotonics, Год журнала: 2024, Номер 11(S1)
Опубликована: Янв. 31, 2024
SignificanceDeep learning enables label-free all-optical biopsies and automated tissue classification. Endoscopic systems provide intraoperative diagnostics to deep speed up treatment without harmful removal. However, conventional multi-core fiber (MCF) endoscopes suffer from low resolution artifacts, which hinder tumor diagnostics.AimWe introduce a method enable unpixelated, high-resolution imaging through given MCF with diameter of around 0.65 mm arbitrary core arrangement inhomogeneous transmissivity.ApproachImage reconstruction is based on the digital twin concept single-reference-based simulation optical properties transfer small experimental dataset biological tissue. The reference provided physical information about during training processes.ResultsFor simulated data, hallucination caused by inhomogeneity was eliminated, averaged peak signal-to-noise ratio structural similarity were increased 11.2 dB 0.20 23.4 0.74, respectively. By learning, metrics independent test images experimentally acquired glioblastoma ex vivo can reach 31.6 0.97 14 fps computing speed.ConclusionsWith proposed approach, single image required in pre-training stage laborious acquisition data bypassed. Validation cryosections only 50 pairs showed capability for retrieval high clinical feasibility.
Язык: Английский
Процитировано
6
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Июнь 14, 2024
Abstract Two-photon voltage imaging has long been heralded as a transformative approach capable of answering many long-standing questions in modern neuroscience. However, exploiting its full potential requires the development novel approaches well suited to photophysical properties genetically encoded indicators. We demonstrate that parallel excitation developed for scanless two-photon photostimulation enable high-SNR imaging. use whole-cell patch-clamp electrophysiology perform thorough characterization using three illumination and lasers with different repetition rates wavelengths. recordings high-frequency spike trains sub-threshold depolarizations from neurons expressing soma-targeted indicator JEDI-2P-Kv. Using low repetition-rate laser, we multi-cell up fifteen targets simultaneously. co-express JEDI-2P-Kv channelrhodopsin ChroME-ST capitalize on their overlapping absorption spectra simultaneously evoke image action potentials single laser source. also vivo multiple cells 250 µm deep barrel cortex head-fixed, anaesthetised mice.
Язык: Английский
Процитировано
6
Neurophotonics, Год журнала: 2024, Номер 11(03)
Опубликована: Март 8, 2024
SignificanceThe function of the hippocampus in behavior and cognition has long been studied primarily through electrophysiological recordings from freely moving rodents. However, application optical recording methods, particularly multiphoton fluorescence microscopy, last decade or two dramatically advanced our understanding hippocampal function. This article provides a comprehensive overview techniques biological findings obtained imaging neural circuits.AimThis review aims to summarize discuss recent technical advances circuits accumulated knowledge gained this technology.ApproachFirst, we provide brief various its advantages, drawbacks, associated key innovations practices. Then, large body by region (CA1 dentate gyrus), cell type (pyramidal neurons, inhibitory interneurons, glial cells), cellular compartment (dendrite axon).ResultsMultiphoton is performed under head-fixed conditions can reveal detailed mechanisms circuit operation owing high spatial resolution specificity. As lies deep below cortex, requires elaborate methods. These include cannula implantation, microendoscopy, use long-wavelength light sources. Although many studies have focused on dorsal CA1 pyramidal cells, other local inter-areal circuitry elements also helped more picture information processing circuits. Imaging mouse models Alzheimer's disease brain disorders such as autism spectrum disorder contributed greatly their pathophysiology.ConclusionsMultiphoton revealed much regarding region-, cell-type-, pathway-specific dysfunction health disease. Future technological will allow further illustration operating principle via large-scale, high-resolution, multimodal, minimally invasive imaging.
Язык: Английский
Процитировано
5
Glia, Год журнала: 2024, Номер 72(11), С. 1955 - 1973
Опубликована: Июль 12, 2024
Abstract Noradrenaline (norepinephrine) is known to modulate many physiological functions and behaviors. In this study, we tested what extent astrocytes, a type of glial cell, participate in noradrenergic signaling mouse primary visual cortex (V1). Astrocytes are essential partners neurons the central nervous system. They brain homeostasis, but also dynamically regulate neuronal activity, notably by relaying regulating neuromodulator signaling. Indeed, astrocytes express receptors for multiple neuromodulators, including noradrenaline, which involved remains unclear. To test whether neuromodulation mice, employed both short hairpin RNA mediated knockdown as well pharmacological manipulation major noradrenaline receptor α1A‐adrenoreceptor. Using acute slices, found that astrocytic α1A‐adrenoreceptor subtype contributes generation large intracellular Ca 2+ signals generally thought underlie astrocyte function. if reduced affected function circuits V1, used patch‐clamp field potential recordings. These revealed through important not only synaptic activity plasticity potentiation responses information processing.
Язык: Английский
Процитировано
5
Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(51)
Опубликована: Окт. 12, 2023
Abstract To interrogate neural circuits and crack their codes, in vivo brain activity imaging must be combined with spatiotemporally precise stimulation three dimensions using genetic or pharmacological specificity. This challenge requires deep penetration focusing as provided by infrared light multiphoton excitation, has promoted two‐photon photopharmacology optogenetics. However, three‐photon remains to demonstrated. We report the regulation of neuronal zebrafish larvae excitation a photoswitchable muscarinic agonist at 50 pM, billion‐fold lower concentration than used for uncaging, mid‐infrared 1560 nm, longest reported photoswitch wavelength. Robust, physiologically relevant photoresponses allow modulating wild‐type animals spatiotemporal precision. Computational calculations predict that azobenzene‐based ligands have high absorption cross‐section can directly pulsed light. The expansion pharmacology will deeply impact basic neurobiology neuromodulation phototherapies.
Язык: Английский
Процитировано
11
Advanced Healthcare Materials, Год журнала: 2024, Номер 14(4)
Опубликована: Сен. 27, 2024
Transparent microelectrode arrays have proven useful in neural sensing, offering a clear interface for monitoring brain activity without compromising high spatial and temporal resolution. The current landscape of transparent electrode technology faces challenges developing durable, highly electrodes while maintaining low impedance prioritizing scalable processing fabrication methods. To address these limitations, we introduce artifact-resistant MXene optimized spatiotemporal resolution recording activity. With 60% transmittance at 550 nm, enable simultaneous imaging electrophysiology multimodal mapping. Electrochemical characterization shows 563 ± 99 kΩ 1 kHz charge storage capacity 58 mC cm⁻² chemical doping. In vivo experiments rodent models demonstrate the arrays' functionality performance. model chemically-induced epileptiform activity, tracked ictal wavefronts via calcium simultaneously seizure onset. rat barrel cortex, recorded multi-unit across cortical depths, showing feasibility high-frequency electrophysiological transparency optical absorption properties Ti₃C₂Tx microelectrodes high-quality recordings light-based stimulation contamination from light-induced artifacts.
Язык: Английский
Процитировано
3
NeuroImage, Год журнала: 2022, Номер 264, С. 119735 - 119735
Опубликована: Ноя. 5, 2022
To improve 'bench-to-bedside' translation, it is integral that knowledge flows bidirectionally—from animal models to humans, and vice versa. This requires common analytical frameworks, as well open software data sharing practices. We share a new pipeline (and test dataset) for the preprocessing of wide-field optical fluorescence imaging data—an emerging mode applicable in models—as results from functional connectivity graph theory analysis inspired by recent work human neuroimaging field. The approach demonstrated using dataset comprised two test-cases: (1) animals imaged during awake anesthetized conditions with excitatory neurons labeled, (2) different genetically encoded fluorescent labels target either or inhibitory interneuron subtypes. Both seed-based measures (global efficiency, transitivity, modularity, characteristic path-length) are shown be useful quantifying differences between wakefulness states cell populations. Wakefulness state type show widespread effects on canonical network variable frequency band dependence. Differences interneurons observed, somatostatin expressing notably dissimilar parvalbumin vasoactive polypeptide cells. In sum, we demonstrate our can used examine brain cell-type mesoscale data, aiding translational neuroscience efforts. line science practices, freely release encourage other efforts community.
Язык: Английский
Процитировано
15