BlueBerry: Closed-loop wireless optogenetic manipulation in freely moving animals

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Abstract Optogenetics is a powerful tool for neuroscientists, enabling manipulations of neuronal activity to explore their effects on behavior. Recent advances in wireless technology enabled the development novel remotely controlled optogenetic devices, thereby promising facilitate study more complex behaviors, including social interactions. Yet, studying such behaviors real time and automated manner large scale naturalistic settings or with multiple animals remains challenging. In addition, many published devices can only be reproduced labs having specific engineering skills fabrication facilities are thus not accessible larger neuroscience community. Here we introduce BlueBerry, an open-source, multi-channel device that assembled off shelf components. The lightweight (1.4 g) design robust long-range communication makes Blueberry ideal behavior-triggered studies interacting freely large-scale setting. We show how BlueBerry used guide decision making during spatial navigation demonstrate first dynamics rapidly altered groups mice by triggering behavior closed-loop optogenetics.

Язык: Английский

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Mesoscale functional architecture in medial posterior parietal cortex

Опубликована: Фев. 7, 2025

The posterior parietal cortex ( PPC ) in mice has various functions including multisensory integration 1–3 , vision-guided behaviors 4–6 working memory 7–13 and posture control 14,15 . However, an integrated understanding of these their cortical localizations around the higher visual areas HVAs ), not been completely elucidated. Here we simultaneously imaged activity thousands neurons within a 3 x mm 2 field-of-view, eight PPC, during behavior with two-photon mesoscope 16 Mice performed both task choice history-dependent task, imaging results revealed distinct, localized, behavior-related two medial areas. Neurons anteromedial AM HVA responded to vision information, thus is locus association between channels. By contrast, anterior A stores history sequential dynamics represents posture. Mesoscale correlation analysis on intertrial variability neuronal demonstrated that area shared fluctuations primary somatosensory area, while exhibited diverse, area-dependent interactions. Pairwise interarea interactions among were precisely predicted by anatomical input correlations, exception some global Thus, distinct modules, AM, which each have distinctive modes communication. These modules can serve separate higher-order functions: for transmission information posture, movement, memory; cognitive locally processed signals.

Язык: Английский

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Mesoscale functional architecture in medial posterior parietal cortex

Опубликована: Фев. 7, 2025

The posterior parietal cortex ( PPC ) in mice has various functions including multisensory integration 1–3 , vision-guided behaviors 4–6 working memory 7–13 and posture control 14,15 . However, an integrated understanding of these their cortical localizations around the higher visual areas HVAs ), not been completely elucidated. Here we simultaneously imaged activity thousands neurons within a 3 x mm 2 field-of-view, eight PPC, during behavior with two-photon mesoscope 16 Mice performed both task choice history-dependent task, imaging results revealed distinct, localized, behavior-related two medial areas. Neurons anteromedial AM HVA responded to vision information, thus is locus association between channels. By contrast, anterior A stores history sequential dynamics represents posture. Mesoscale correlation analysis on intertrial variability neuronal demonstrated that area shared fluctuations primary somatosensory area, while exhibited diverse, area-dependent interactions. Pairwise interarea interactions among were precisely predicted by anatomical input correlations, exception some global Thus, distinct modules, AM, which each have distinctive modes communication. These modules can serve separate higher-order functions: for transmission information posture, movement, memory; cognitive locally processed signals.

Язык: Английский

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An opponent striatal circuit for distributional reinforcement learning

Nature, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Язык: Английский

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Locomotion-dependent auditory gating to the parietal cortex guides multisensory decisions

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 7, 2025

Decision-making in mammals fundamentally relies on integrating multiple sensory inputs, with conflicting information resolved flexibly based a dominant modality. However, the neural mechanisms underlying state-dependent changes dominance remain poorly understood. Our study demonstrates that locomotion mice shifts auditory-dominant decisions toward visual during audiovisual conflicts. Using circuit-specific calcium imaging and optogenetic manipulations, we found weakened representation posterior parietal cortex (PPC) leads to stationary mice. Prolonged locomotion, however, promotes by inhibiting auditory cortical neurons projecting PPC (ACPPC). This shift is mediated secondary motor (M2AC), which specifically inhibit ACPPC without affecting projections striatum (ACSTR). findings reveal circuit gating association depending states, providing insights into multisensory decision-making. Neural basis of decision-making not fully Here authors show from activating inhibits output cortex. reveals how shapes perception.

Язык: Английский

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New information triggers prospective codes to adapt for flexible navigation

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Май 24, 2025

Язык: Английский

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Hippocampal sequences span experience relative to rewards

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 28, 2023

Hippocampal place cells fire in sequences that span spatial environments and non-spatial modalities, suggesting hippocampal activity can anchor to the most behaviorally salient aspects of experience. As reward is a highly event, we hypothesized rewards. To test this, performed two-photon imaging CA1 neurons as mice navigated virtual with changing hidden locations. When moved, firing fields subpopulation moved same relative position respect reward, constructing sequence reward-relative spanned entire task structure. The density these increased experience additional were recruited population. Conversely, largely separate maintained spatially-based code. These findings thus reveal ensembles flexibly encode multiple reference frames, reflecting structure

Язык: Английский

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An entorhinal-like region in food-caching birds

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Янв. 6, 2023

The mammalian entorhinal cortex routes inputs from diverse sources into the hippocampus. This information is mixed and expressed in activity of many specialized cell types, which are considered indispensable for hippocampal function. However, functionally similar hippocampi exist even non-mammals that lack an obvious cortex, or generally any layered cortex. To address this dilemma, we mapped extrinsic connections chickadees, whose used remembering numerous food caches. We found a well-delineated structure these birds topologically to interfaces between hippocampus other pallial regions. Recordings revealed entorhinal-like activity, including border multi-field grid-like cells. These cells were localized subregion predicted by anatomical mapping match dorsomedial Our findings uncover physiological equivalence vastly different brains, suggesting fundamental nature computations

Язык: Английский

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Arousal and Locomotion Differently Modulate Activity of Somatostatin Neurons across Cortex

eNeuro, Год журнала: 2023, Номер 10(5), С. ENEURO.0136 - 23.2023

Опубликована: Май 1, 2023

Arousal powerfully influences cortical activity, in part by modulating local inhibitory circuits. Somatostatin (SOM)-expressing interneurons are particularly well situated to shape population activity response shifts arousal, yet the relationship between arousal state and SOM has not been characterized outside of sensory cortex. To determine whether is similarly modulated behavioral across different levels processing hierarchy, we compared modulation SOM-expressing neurons auditory cortex (AC), a primary region, posterior parietal (PPC), an association-level region cortex, mice. Behavioral differently AC PPC. In PPC, transitions high were accompanied large increases full PPC neural population, especially neurons. AC, led more subtle changes overall as individual Non-SOM could be either positively or negatively during states. The coding information was enhanced periods but Our findings suggest unique relationships circuits which may tailored roles specific regions control behavior.

Язык: Английский

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Circuit mechanisms of navigation strategy learning in mice

Current Biology, Год журнала: 2023, Номер 34(1), С. 79 - 91.e4

Опубликована: Дек. 14, 2023

Navigation tasks involve the gradual selection and deployment of increasingly effective searching procedures to reach targets. The brain mechanisms underlying such complex behavior are poorly understood, but their elucidation might provide insights into systems linking exploration decision making in learning. Here, we developed a trial-by-trial goal-related search strategy analysis as mice learned navigate identical water mazes encompassing distinct rules monitored process throughout We found that navigation learning involved following three phases: an early phase during which maze-specific strategies deployed minority trials, second preferential increasing one strategy, final commitment this only. maze phases were affected differently by inhibition retrosplenial cortex (RSC), dorsomedial striatum (DMS), or dorsolateral (DLS). Through region-specific inactivation experiments gain-of-function involving activation learning-related cFos+ ensembles, unraveled how relates these sequential processes. RSC is critically important for selection, DMS mediates deployment, DLS ensures consistency Notably, specific ensembles was sufficient direct (RSC) (DMS) different maze. Our results establish approach dissect unsupervised processes suggest involves evidence-based direction RSC, reinforcement-modulated through DMS, online guidance DLS.

Язык: Английский

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