bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 8, 2024
Abstract
Although
many
methods
for
automated
fluorescent-labeled
cell
detection
have
been
proposed,
not
all
of
them
assume
a
highly
inhomogeneous
background
arising
from
complex
biological
structures.
Here,
we
propose
an
algorithm
that
accounts
and
subtracts
the
by
avoiding
high-intensity
pixels
in
blur
filtering
calculation.
Cells
were
detected
intensity
thresholding
background-subtracted
image,
algorithm’s
performance
was
tested
on
NeuN-
c-Fos-stained
images
mouse
prefrontal
cortex
hippocampal
dentate
gyrus.
In
addition,
applications
c-Fos
positive
counting
quantification
expression
level
double-labeled
cells
demonstrated.
Our
method
after
assumption
(ADABA)
offers
advantage
high-throughput
unbiased
analysis
regions
with
structures
produce
background.
Highlights
-
We
proposed
to
subtract
pattern.
(79/85)
automatically
image.
(71/85)
The
results
corresponded
manual
detection.
(73/85)
Detection
IEG
overlapping
neural
marker
(85/85)
Science,
Год журнала:
2024,
Номер
385(6715), С. 1298 - 1305
Опубликована: Сен. 19, 2024
For
several
decades,
although
studies
of
rat
physiology
and
behavior
have
abounded,
research
on
emotions
has
been
limited
in
scope
to
fear,
anxiety,
pain.
Converging
evidence
for
the
capacity
many
species
share
others’
affective
states
emerged,
sparking
interest
empathic
capacities
rats.
Recent
demonstrated
that
rats
are
a
highly
cooperative
motivated
by
distress
prosocial
actions,
such
as
opening
door
or
pulling
chain
release
trapped
conspecifics.
Studies
affect,
cognition,
neural
function
provide
compelling
some
represent
needs,
instrumentally
act
improve
their
well-being,
thus
capable
forms
targeted
helping.
Rats’
complex
abilities
raise
importance
integrating
new
measures
well-being
into
scientific
research.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
Abstract
Expression
of
immediate
early
genes
(IEGs)
is
critical
for
memory
formation
and
has
been
widely
used
to
identify
the
neural
substrate
traces,
termed
engram
cells.
However,
types
IEG
have
relatively
not
distinguished
in
identifying
cells,
as
there
limited
knowledge
about
extent
which
different
IEGs
are
selectively
or
concurrently
involved
engram.
To
address
this
question,
we
investigated
combinative
expression
c-Fos,
Arc,
Npas4
proteins
using
immunohistochemistry
following
aversive
rewarding
experiences
across
subregions
prefrontal
cortex
(PFC),
basolateral
amygdala
(BLA),
hippocampal
dentate
gyrus
(DG),
retrosplenial
(RSC).
Using
an
automated
cell
detection
algorithm,
found
that
patterns
Npas4,
Arc
varied
brain
areas,
with
a
higher
increase
expressing
cells
PFC
posterior
BLA
than
DG.
The
patterns,
along
their
learning-induced
changes,
also
differed
areas;
co-expression
increased
learning
whereas
was
less
pronounced
DG
RSC.
Furthermore,
demonstrate
area-to-area
functional
connectivity
networks
were
extracted
by
IEGs.
These
findings
provide
insights
into
how
combinations
will
contribute
deeper
understanding
significance
IEG-tagged
Brain Research Bulletin,
Год журнала:
2025,
Номер
222, С. 111247 - 111247
Опубликована: Фев. 5, 2025
Significant
advancements
have
been
made
in
the
treatment
of
psychotic
disorders,
yet
current
pharmacotherapy
remains
inadequate.
Symptoms
related
to
misinterpretation
reality
are
crucial
for
diagnosis
but
pose
challenges
preclinical
research.
In
humans,
a
Mirror-Gazing
test
is
used
examine
abnormal
self-experience
and
predict
risk
schizophrenia.
To
address
this,
we
developed
task
evaluate
anomalous
subjective
experiences
rats
using
Mirror-Preference
test.
Here
demonstrate
that
naive
show
preference
mirror
chamber,
which
was
followed
by
significant
habituation
over
series
trials.
subsequent
tests,
utilized
dimmed
lighting
net-covered
mirrors
induce
incomplete
images.
Acute
stimulation
with
amphetamine
(AMPH,
3
mg/kg,
i.p.)
further
increased
mirror.
model
psychosis
induced
chronic
AMPH
administration,
showed
fewer
shorter
interactions
maintained
their
chamber
where
psychotic-like
animals
were
given
additional
(1.5
before
testing.
Here,
surprising
reversal
observed,
along
decreased
frequency
duration
contact,
suggesting
avoidance.
The
AMPH-presensitized
also
exhibited
hyperlocomotion
elevated
anxiety,
indicative
behaviour.
Although
self-recognition
rodents
debatable,
recent
studies
suggest
they
can
discriminate
We
propose
limited
visual
perception
meets
brain
monoamine
sensitization
may
trigger
illusions
as
part
state
rats.
This
novel
approach
be
intervention
strategies
Brain and Behavior,
Год журнала:
2025,
Номер
15(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Background
Since
its
discovery
in
the
late
20th
century,
research
on
mirror
neurons
has
become
a
pivotal
area
neuroscience,
linked
to
various
cognitive
and
social
functions.
This
bibliometric
analysis
explores
trajectory,
key
topics,
future
trends
field
of
neuron
research.
Methods
We
searched
Web
Science
Core
Collection
(WoSCC)
database
for
publications
from
1996
2024
Statistical
visualization
analyses
were
performed
using
CiteSpace
VOSviewer.
Results
Publication
output
peaked
2013
remained
active.
High‐impact
journals
such
as
Science,
Brain,
Neuron,
PNAS
,
NeuroImage
frequently
feature
findings
system,
including
distribution,
neural
coding,
roles
intention
understanding,
affective
empathy,
motor
learning,
autism,
neurological
disorders.
Keyword
clustering
reveals
major
directions
neurostimulation,
whereas
burst
detection
underscores
emerging
significance
brain‐computer
interfaces
(BCIs).
Research
methodologies
have
been
evolving
traditional
electrophysiological
recordings
advanced
techniques
functional
magnetic
resonance
imaging,
transcranial
stimulation,
BCIs,
highlighting
dynamic,
multidisciplinary
progression.
Conclusions
study
identifies
areas
associated
with
anticipates
that
work
will
integrate
artificial
intelligence,
clinical
interventions,
novel
neuroimaging
techniques,
providing
new
perspectives
complex
socio‐cognitive
issues
their
applications
both
basic
science
practice.
Animals,
Год журнала:
2024,
Номер
14(23), С. 3384 - 3384
Опубликована: Ноя. 25, 2024
Body
size
awareness
is
the
ability
of
animals
to
consider
their
bodies
when
interacting
with
environmental
objects.
It
one
manifestations
body
self-awareness.
We
studied
in
laboratory
rats
Wistar
strain.
In
experiment,
had
pass
through
three
holes
a
partition
reach
bait.
each
trial,
shape,
size,
and
position
varied.
The
task
first
experiment
was
establish
whether
prefer
larger
holes,
given
that
all
were
penetrable
for
bodies.
second
can
choose
only
hole
while
other
two
greater
area
but
impenetrable.
results
showed
did
not
show
preference
holes.
make
approach
subsequent
penetration
immediately
into
even
before
tactile
contact
partition.
Thus,
own
solving
penetrating
hole,
which
sign
awareness.
These
enrich
our
general
understanding
phenomenon
