Engineering of electrospun nanofiber scaffolds for repairing brain injury

Engineered Regeneration, Год журнала: 2023, Номер 4(3), С. 289 - 303

Опубликована: Апрель 9, 2023

Язык: Английский

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Perioperative stroke: A perspective on challenges and opportunities for experimental treatment and diagnostic strategies

CNS Neuroscience & Therapeutics, Год журнала: 2022, Номер 28(4), С. 497 - 509

Опубликована: Фев. 27, 2022

Abstract Perioperative stroke is an ischemic or hemorrhagic cerebral event during up to 30 days after surgery. It a feared condition due relatively high incidence, difficulties in timely detection, and unfavorable outcome compared spontaneously occurring stroke. Recent preclinical data suggest that specific pathophysiological mechanisms such as aggravated neuroinflammation contribute the detrimental impact of perioperative Conventional treatment options are limited setting difficult diagnosis medications affecting coagulation may cases. On contrary, chance anticipate cerebrovascular events at time surgery pave way for prevention strategies. This review provides overview on related problems, underlying mechanisms. Based this analysis, we assess experimental treatments including neuroprotective approaches, cell therapies, conditioning medicine strategies regarding their potential use Interestingly, aspects might enable more effective application classical neuroprotection whereas others therapies be use. We also discuss diagnostic augmenting clinical imaging diagnosis. While some have advantages stroke, paucity established guidelines multicenter research initiatives currently limits thorough investigation.

Язык: Английский

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Triad3A-Dependent TLR4 Ubiquitination and Degradation Contributes to the Anti-Inflammatory Effects of Pterostilbene on Vascular Dementia

Journal of Agricultural and Food Chemistry, Год журнала: 2022, Номер 70(19), С. 5896 - 5910

Опубликована: Май 9, 2022

Pterostilbene, a methylated stilbene derived from many plant foods, has significant anti-inflammatory activity. Meanwhile, vascular dementia (VaD) is the second most common subtype of dementia, in which inflammation one major pathogenic contributors. However, protective effect pterostilbene on VaD not well understood. In this work, we investigated and explored its underlying mechanisms using vivo vitro models. Y-maze Morris water maze tests showed pterostilbene-attenuated cognitive impairment mice with bilateral carotid artery occlusion (BCCAO). The hippocampal neuronal death microglial activation BCCAO were also reduced by treatment. Further, inhibited expression TLR4 downstream inflammatory cytokines these mice, similar results observed an oxygen–glucose deprivation reperfusion (OGD/R) BV-2 cell model. addition, OGD/R cells was partially blocked overexpression. Moreover, Triad3A–TLR4 interactions increased following enhanced ubiquitination degradation TLR4, inhibitory Triad3A knockdown OGD/R-stimulated cells. Together, reveal that could reduce Triad3A-mediated might be key target.

Язык: Английский

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The Assessment of Endovascular Therapies in Ischemic Stroke: Management, Problems and Future Approaches

Journal of Clinical Medicine, Год журнала: 2022, Номер 11(7), С. 1864 - 1864

Опубликована: Март 28, 2022

Ischemic stroke accounts for over 80% of all strokes and is one the leading causes mortality permanent disability worldwide. Intravenous administration recombinant tissue plasminogen activator (rt-PA) an approved treatment strategy acute ischemic large arteries within 4.5 h onset, mechanical thrombectomy can be used occlusion up to 24 after onset. Improving diagnostic work treatment, reducing onset-to-needle time urgent radiological access angiographic CT images (angioCT) Magnetic Resonance Imaging (MRI) are real problems many healthcare systems, which limits number patients with good prognosis in world compared results randomized controlled trials. The applied endovascular procedures demonstrated high efficacy, but some cellular mechanisms, following reperfusion, still unknown. Changes morphology function mitochondria associated reperfusion ischemia-reperfusion neuronal death understudied research fields. Moreover, future needed elucidate relationship between continuously refined imaging techniques variable structure or physical properties clot along vascular permeability pleiotropism lesions penumbra, order define targeted preventive promoting long-term health benefits.

Язык: Английский

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Trilobatin promotes angiogenesis after cerebral ischemia–reperfusion injury via SIRT7/VEGFA signaling pathway in rats

Phytotherapy Research, Год журнала: 2022, Номер 36(7), С. 2940 - 2951

Опубликована: Май 10, 2022

Angiogenesis plays a pivotal role in the recovery of neurological function after ischemia stroke. Herein, we investigated effect trilobatin (TLB) on angiogenesis cerebral ischemia-reperfusion injury (CIRI). The TLB CIRI were mouse brain microvascular endothelium bEnd.3 cells and middle artery occlusion (MCAO)-induced rat model. cell proliferation observed using immunofluorescence staining. cycle, expressions cycle-related proteins SIRT 1-7 determined by flow cytometry western blot, respectively. binding affinity with SIRT7 was predicted molecular docking. results showed that concentration-dependently promoted proportion S-phase. significantly increased protein SIRT6, SIRT7, VEGFA, but not affected SIRT1-SIRT5 expressions. Moreover, only dramatically alleviated impairment CIRI, also enhanced post-stroke neovascularization newly formed functional vessels ischemic penumbra. Furthermore, up-regulated CDK4, cyclin D1, VEGFA its receptor VEGFR-2. Intriguingly, directly bound to expression at day 28. Our findings reveal promotes endothelial proliferation, facilitates via mediating SIRT7/VEGFA signaling pathway rats. Therefore, might be novel restorative agent rescue

Язык: Английский

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