Polymer-based bioconjugation: engineering biomolecules and nanocarriers for advanced applications
International Journal of Polymeric Materials,
Год журнала:
2025,
Номер
unknown, С. 1 - 21
Опубликована: Март 19, 2025
Bioconjugation,
a
unique
approach,
is
commonly
used
to
enhance
the
biopharmaceutical
properties
of
bioactive
while
also
allowing
for
its
spatial
and
temporal
distribution.
The
technique
opens
up
new
possibilities
delivering
peptides,
lipids,
oligonucleotides,
enzymes
are
included
in
bioconjugate.
Site-specific
delivery
can
be
accomplished
through
converting
conjugates
into
inactive
prodrugs
forming
polymer
drug
combination
that
prone
cleavage
by
specific
pH
or
enzymes.
Such
have
significant
impact
on
cellular
entry
mechanisms,
pharmacokinetic
disposition,
targeting.
conjugated
carriers
deliver
medicinal
medications
visceral
organs
like
brain
intestines.
biomaterials
offering
novel
systems
precisely
appropriately
carry
drugs
target
organs.
Noteworthy,
advancements
bioconjugated
nanoparticles
biosensing
bioimaging
include
cell
labeling,
DNA
identification,
separation,
recombination,
applications
protection.
Only
recent
times
these
tailor-made
polymers
acquired
traction
various
therapies
with
minimal
side
effects.
current
analysis
comprehensively
examines
bioconjugates
their
implications
toward
selective
This
review
associated
conjugates,
which
could
serve
as
exciting
vehicles.
Язык: Английский
Chitosan nanoparticles of imatinib mesylate coated with TPGS for the treatment of colon cancer: In-vivo & in-vitro studies
Carbohydrate Polymers,
Год журнала:
2024,
Номер
348, С. 122935 - 122935
Опубликована: Ноя. 4, 2024
Язык: Английский
Advances in Nanomedicine for Retinal Drug Delivery: Overcoming Barriers and Enhancing Therapeutic Outcomes
Journal of drug targeting,
Год журнала:
2024,
Номер
unknown, С. 1 - 49
Опубликована: Дек. 18, 2024
Nanomedicine
offers
a
promising
avenue
for
improving
retinal
drug
delivery,
effectively
addressing
challenges
associated
with
ocular
diseases
like
age-related
macular
degeneration
and
diabetic
retinopathy.
Nanoparticles,
their
submicron
size
customizable
surface
properties,
enable
enhanced
permeability
retention
within
tissues,
supporting
sustained
release
minimizing
systemic
side
effects.
Nanostructured
scaffolds
further
provide
supportive
environment
cell
growth
tissue
regeneration,
crucial
treating
degenerative
conditions.
Additionally,
advanced
nanodevices
facilitate
real-time
monitoring
controlled
release,
marking
significant
progress
in
therapy.
This
study
reviews
recent
advancements
nanomedicine
critically
analyzing
design
innovations,
therapeutic
benefits,
limitations
of
these
systems.
By
advancing
nanotechnology
integration
therapies,
this
field
holds
strong
potential
overcoming
current
barriers,
ultimately
patient
outcomes
quality
life.
Язык: Английский
Insight Into the Molecular Parameters of PEI Promoting an Efficient Gene Delivery into Cells
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 3, 2024
Abstract
The
development
of
natural
or
synthetic
polycations
able
to
interact
with
nucleic
acids
and
condense
them
into
nanoparticles
known
as
polyplexes,
faces
several
unresolved
challenges
at
the
cellular
level.
Key
issues
include
intracellular
trafficking
endosomal
escape
release
cytosol,
which
are
considered
major
bottlenecks
for
efficient
protein
expression.
Here,
we
aim
gaining
fundamental
insights
stability
polyplexes
in
biological
media
their
uptake
trafficking,
while
correlating
data
expression
reporter
both
molecular
characteristics
various
poly(ethylenimines)
(PEI)
physicochemical
PEI/peGFP-C3
polyplexes.
For
this,
chosen
four
samples
PEI,
selected
a
model
polycation,
different
weights
(Mw
=
0.8,
20,
25
60
kg/mol)
structures
(linear
branched).
We
found
that
vitro
vivo
cell
internalization
transfection
efficiency
is
dependent
on
variation
polycation
Mw
structure,
well
intrinsic
properties
such
charge
ratio
(R=[N
+
]/[P
−
]).
A
relation
between
percentage
positive
cells
green
fluorescent
(GFP)
amount
internalized
acid
(cyanine
5-peGFP-C3)
allowed
revealing
PEI
promoting
higher
GFP
HEK293T
cells.
In
long
term,
outcome
this
work
will
be
propose
guidelines
help
design
more
effective,
less
cytotoxic
non-viral
gene
carriers
great
potential
new
therapeutic
applications.
Язык: Английский