Insight Into the Molecular Parameters of PEI Promoting an Efficient Gene Delivery into Cells DOI Open Access
Paulina González, Sylvain Tranchimand,

Manon Rochedy

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract The development of natural or synthetic polycations able to interact with nucleic acids and condense them into nanoparticles known as polyplexes, faces several unresolved challenges at the cellular level. Key issues include intracellular trafficking endosomal escape release cytosol, which are considered major bottlenecks for efficient protein expression. Here, we aim gaining fundamental insights stability polyplexes in biological media their uptake trafficking, while correlating data expression reporter both molecular characteristics various poly(ethylenimines) (PEI) physicochemical PEI/peGFP-C3 polyplexes. For this, chosen four samples PEI, selected a model polycation, different weights (Mw = 0.8, 20, 25 60 kg/mol) structures (linear branched). We found that vitro vivo cell internalization transfection efficiency is dependent on variation polycation Mw structure, well intrinsic properties such charge ratio (R=[N + ]/[P − ]). A relation between percentage positive cells green fluorescent (GFP) amount internalized acid (cyanine 5-peGFP-C3) allowed revealing PEI promoting higher GFP HEK293T cells. In long term, outcome this work will be propose guidelines help design more effective, less cytotoxic non-viral gene carriers great potential new therapeutic applications.

Language: Английский

Polymer-based bioconjugation: engineering biomolecules and nanocarriers for advanced applications DOI
Pratikeswar Panda,

Sangita Ranee Gouda,

Sanjib Mohanty

et al.

International Journal of Polymeric Materials, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 21

Published: March 19, 2025

Bioconjugation, a unique approach, is commonly used to enhance the biopharmaceutical properties of bioactive while also allowing for its spatial and temporal distribution. The technique opens up new possibilities delivering peptides, lipids, oligonucleotides, enzymes are included in bioconjugate. Site-specific delivery can be accomplished through converting conjugates into inactive prodrugs forming polymer drug combination that prone cleavage by specific pH or enzymes. Such have significant impact on cellular entry mechanisms, pharmacokinetic disposition, targeting. conjugated carriers deliver medicinal medications visceral organs like brain intestines. biomaterials offering novel systems precisely appropriately carry drugs target organs. Noteworthy, advancements bioconjugated nanoparticles biosensing bioimaging include cell labeling, DNA identification, separation, recombination, applications protection. Only recent times these tailor-made polymers acquired traction various therapies with minimal side effects. current analysis comprehensively examines bioconjugates their implications toward selective This review associated conjugates, which could serve as exciting vehicles.

Language: Английский

Citations

0
Chitosan nanoparticles of imatinib mesylate coated with TPGS for the treatment of colon cancer: In-vivo & in-vitro studies DOI
Darshan Bhirud, Sankha Bhattacharya, Harshvardhan Raval

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 348, P. 122935 - 122935

Published: Nov. 4, 2024

Language: Английский

Citations

2
Advances in Nanomedicine for Retinal Drug Delivery: Overcoming Barriers and Enhancing Therapeutic Outcomes DOI
Pratikeswar Panda, Sanjib Mohanty,

Sangita Ranee Gouda

et al.

Journal of drug targeting, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 49

Published: Dec. 18, 2024

Nanomedicine offers a promising avenue for improving retinal drug delivery, effectively addressing challenges associated with ocular diseases like age-related macular degeneration and diabetic retinopathy. Nanoparticles, their submicron size customizable surface properties, enable enhanced permeability retention within tissues, supporting sustained release minimizing systemic side effects. Nanostructured scaffolds further provide supportive environment cell growth tissue regeneration, crucial treating degenerative conditions. Additionally, advanced nanodevices facilitate real-time monitoring controlled release, marking significant progress in therapy. This study reviews recent advancements nanomedicine critically analyzing design innovations, therapeutic benefits, limitations of these systems. By advancing nanotechnology integration therapies, this field holds strong potential overcoming current barriers, ultimately patient outcomes quality life.

Language: Английский

Citations

1
Insight Into the Molecular Parameters of PEI Promoting an Efficient Gene Delivery into Cells DOI Open Access
Paulina González, Sylvain Tranchimand,

Manon Rochedy

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract The development of natural or synthetic polycations able to interact with nucleic acids and condense them into nanoparticles known as polyplexes, faces several unresolved challenges at the cellular level. Key issues include intracellular trafficking endosomal escape release cytosol, which are considered major bottlenecks for efficient protein expression. Here, we aim gaining fundamental insights stability polyplexes in biological media their uptake trafficking, while correlating data expression reporter both molecular characteristics various poly(ethylenimines) (PEI) physicochemical PEI/peGFP-C3 polyplexes. For this, chosen four samples PEI, selected a model polycation, different weights (Mw = 0.8, 20, 25 60 kg/mol) structures (linear branched). We found that vitro vivo cell internalization transfection efficiency is dependent on variation polycation Mw structure, well intrinsic properties such charge ratio (R=[N + ]/[P − ]). A relation between percentage positive cells green fluorescent (GFP) amount internalized acid (cyanine 5-peGFP-C3) allowed revealing PEI promoting higher GFP HEK293T cells. In long term, outcome this work will be propose guidelines help design more effective, less cytotoxic non-viral gene carriers great potential new therapeutic applications.

Language: Английский

Citations

0