Efficient genotype‐independent cotton genetic transformation and genome editing

Journal of Integrative Plant Biology, Год журнала: 2022, Номер 65(4), С. 907 - 917

Опубликована: Дек. 7, 2022

Cotton (Gossypium spp.) is one of the most important fiber crops worldwide. In last two decades, transgenesis and genome editing have played roles in cotton improvement. However, genotype dependence key bottlenecks generating transgenic gene-edited plants through either particle bombardment or Agrobacterium-mediated transformation. Here, we developed a shoot apical meristem (SAM) cell-mediated transformation system (SAMT) that allowed recalcitrant genotypes including widely grown upland hirsutum), Sea island barbadense), Asiatic arboreum). Through SAMT, successfully introduced foreign genes, GFP RUBY, into SAM cells some genotypes. Within 2-3 months, adventitious shoots generated from axillary zone could be recovered whole plants. The fluorescent signal betalain accumulation observed various tissues GFP- RUBY-positive plants, as well their progenies, indicating transgenes were stably integrated transmitted to next generation. Furthermore, using edited with inheritable targeted mutagenesis GhPGF GhRCD1 genes CRISPR/Cas9-mediated editing. summary, established SAMT here this study bypasses embryogenesis process during tissue culture conventional procedure significantly accelerates generation for genetic improvement varieties.

Язык: Английский

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What factors control plant height?

Journal of Integrative Agriculture, Год журнала: 2024, Номер 23(6), С. 1803 - 1824

Опубликована: Март 22, 2024

Plant height (PH) is one of the most important components a plant ideotype, affecting biomass, yields, lodging resistance, and ability to use mechanized harvesting. As many complex pathways controlling growth development remain poorly understood, we are still unable obtain ideal plants solely through breeding efforts. can be influenced by genotypes, hormone regulations, environmental conditions, interaction with other plants. Here, comprehensively review factors PH, including regulation PH-related processes, genetics QTLs contributing hormone-regulated molecular mechanisms for PH. Additionally, symbiotic influence grafting on PH was discussed focusing gene expressions genetics. Finally, proposed strategies how apply recent findings better highlighted some knowledge gaps, suggested potential directions future studies.

Язык: Английский

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Building beauty: Understanding how hormone signaling regulates petal patterning and morphogenesis

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

The corolla of flowering plants provides pivotal functions for the reproduction angiosperms, directly impacting fitness individuals. Different petal shapes and patterns contribute to these and, thus, participate in production morphological diversity emergence new species. During morphogenesis, coordination cell fate specification, division, expansion is coherent robust across blade set according proximo-distal, medio-lateral, abaxial-adaxial axes. However, mechanisms specifying polarity controlling behavior a position-dependent manner as petals develop remain poorly understood. In this review, we draw parallels with other evolutionarily related plant lateral organs such leaves argue that hormones likely play central, yet largely unexplored, roles coordination. By examining development Arabidopsis frame what are knowns unknowns contributions morphogenesis patterning. Finally, using emerging model organisms can provide invaluable information tackle questions have long remained unanswered, broadening our understanding by allowing us investigate tinkering phytohormone signaling through an evolutionary lens.

Язык: Английский

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Looking beyond the gene network – metabolic and mechanical cell drivers of leaf morphogenesis

Journal of Cell Science, Год журнала: 2022, Номер 135(8)

Опубликована: Апрель 15, 2022

ABSTRACT The above-ground organs in plants display a rich diversity, yet they grow to characteristic sizes and shapes. Organ morphogenesis progresses through sequence of key events, which are robustly executed spatiotemporally as an emerging property intrinsic molecular networks while adapting various environmental cues. This Review focuses on the multiscale control leaf morphogenesis. Beyond list known genetic determinants underlying growth shape, we focus instead novel mechanisms metabolic biomechanical regulations that coordinate plant cell non-cell-autonomously. reveals how metabolism mechanics not solely passive outcomes regulation but play instructive roles Such integrative view also extends fluctuating cues evolutionary adaptation. synthesis calls for more balanced morphogenesis, where shapes considered from standpoints geometry, genetics, energy mechanics, properties cellular expression these different properties.

Язык: Английский

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Epigenetic regulation in the shoot apical meristem

Current Opinion in Plant Biology, Год журнала: 2022, Номер 69, С. 102267 - 102267

Опубликована: Авг. 16, 2022

Epigenetic mechanisms form the basis of cellular memory, developmental decisions, and immune system that defends against transposons viruses. Organs develop from shoot apical meristem (SAM) to shape plant's areal phenotype, stem cells in SAM serve as a functional germline. While many details on regulation cell pool size, organ initiation, patterning at periphery are known, we know surprisingly little about molecular characteristics cells, including their epigenome how it changes during development. Here, summarize information epigenetic selected genes necessary for maintenance. As recent evidence suggests might be hotspot transposon activation, discuss this aspect control speculate maintain flexibility response or environmental cues.

Язык: Английский

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HAM Gene Family and Shoot Meristem Development

Frontiers in Plant Science, Год журнала: 2021, Номер 12

Опубликована: Дек. 20, 2021

Land plants develop highly diversified shoot architectures, all of which are derived from the pluripotent stem cells in apical meristems (SAMs). As sustainable resources for continuous organ formation aboveground tissues, SAMs play an important role determining plant yield and biomass production. In this review, we summarize recent advances understanding one group key regulators – HAIRY MERISTEM (HAM) family GRAS domain proteins meristems. We highlight functions HAM members dictating cell initiation proliferation, signaling cascade that shapes expression domains meristems, conservation diversification land plants. also discuss future directions potentially lead to a more comprehensive view gene homeostasis

Язык: Английский

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Meristem Initiation and de novo Stem Cell Formation

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Апрель 26, 2022

Plant aerial development relies on meristem activity which ensures main body plant axis during life. While the shoot apical (SAM) formed in embryo only contributes to stem, branched structure observed many plants axillary meristems (AMs) post-embryonically. These AMs initiate from a few cells of leaf axil that retain meristematic characteristics, increase number, and finally organize into similar SAM. In this review, we will discuss recent findings

Язык: Английский

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Understanding Cytokinin: Biosynthesis, Signal Transduction, Growth Regulation, and Phytohormonal Crosstalk under Heavy Metal Stress

Environmental and Experimental Botany, Год журнала: 2024, Номер 228, С. 106025 - 106025

Опубликована: Ноя. 2, 2024

Язык: Английский

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Genome-Wide Identification and Expression Analysis of the Response Regulator (RR) Gene Family in Sacha Inchi

Journal of Plant Growth Regulation, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

Язык: Английский

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The self‐replicating cellular organization of shoot apical meristems

American Journal of Botany, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Apical meristems of land plants have played a fundamental role in the evolution complex shoot architectures. The most common structure apical bryophytes, lycophytes, and ferns is characterized by single cell surrounded spiral derivatives. Despite importance this type meristem organization, it remains unclear how maintained at apex. We analyzed distribution different organizations within data set 205 images representing 91 species ferns. In parallel, we developed mathematical computational model to determine whether structural types observed empirically are predicted from Sachs's division rules; namely, cells divide symmetrically while positioning their new wall right angle parental walls. According our set, only four nature, corresponding dividing along one, two, three, or faces. addition, prevalence diverse plant lineages correlates with shape on which they found. Our based rules indicates that as much six geometrically possible, but dynamically stable for realistic geometries. Simple rules, interpret biophysical constraints assembly preprophase band, may therefore explain cellular organization three major groups plants.

Язык: Английский

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