PAT1-type GRAS-domain proteins control regeneration by activating DOF3.4 to drive cell proliferation in Arabidopsis roots

The Plant Cell, Год журнала: 2023, Номер 35(5), С. 1513 - 1531

Опубликована: Фев. 7, 2023

Abstract Plant roots possess remarkable regenerative potential owing to their ability replenish damaged or lost stem cells. ETHYLENE RESPONSE FACTOR 115 (ERF115), one of the key molecular elements linked this potential, plays a predominant role in activation cell divisions. However, downstream operating machinery driving wound-activated division is largely unknown. Here, we biochemically and genetically identified GRAS-domain transcription factor SCARECROW-LIKE 5 (SCL5) as an interaction partner ERF115 Arabidopsis thaliana. Although nonessential under control growth conditions, SCL5 acts redundantly with related PHYTOCHROME A SIGNAL TRANSDUCTION 1 (PAT1) SCL21 factors activate expression DNA-BINDING ONE FINGER 3.4 (DOF3.4) gene. DOF3.4 wound-inducible ERF115-dependent manner and, turn, activates D3-type cyclin expression. Accordingly, ectopic drives periclinal division, while its cyclins are essential for regeneration root. Our data highlight importance redundant roles SCL5, SCL21, PAT1 processes pinpoint element division.

Язык: Английский

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Positional cues and cell division dynamics drive meristem development and archegonium formation in Ceratopteris gametophytes

Communications Biology, Год журнала: 2022, Номер 5(1)

Опубликована: Июль 1, 2022

Abstract Fern gametophytes are autotrophic and independent of sporophytes, they develop pluripotent meristems that drive prothallus development sexual reproduction. To reveal cellular dynamics during meristem in fern gametophytes, we performed long-term time-lapse imaging determined the real-time lineage, identity division activity each single cell from initiation to establishment Ceratopteris richardii . Our results demonstrate only a few lineages originated marginal layer contribute proliferation, lacks distinguishable central zone or apical with low activity. Within meristem, is cells at more actively dividing than inner cells. Furthermore, triggers differentiation adjacent into egg-producing archegonia position-dependent manner. These findings advance understanding diversified gametophyte land plants.

Язык: Английский

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Network of GRAS transcription factors in plant development, fruit ripening and stress responses

Horticulture Research, Год журнала: 2023, Номер 10(12)

Опубликована: Сен. 27, 2023

The plant-specific family of GRAS transcription factors has been wide implicated in the regulation transcriptional reprogramming associated with a diversity biological functions ranging from plant development processes to stress responses. Functional analyses supported by silico structural and comparative are emerging clarifying regulatory networks their roles. In this review, detailed analysis proteins' structure biochemical features as revealed recent discoveries indicated how these characteristics may impact subcellular location, molecular mechanisms, function. Nomenclature issues classification into different subfamilies diverse species even presence robust genomic resources discussed, particular it affects assumptions Insights mechanisms driving evolution gene genetic epigenetic contributes subfunctionalization provided. Finally, review debates challenges future perspectives on application complex but promising for crop improvement cope environmental transition.

Язык: Английский

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Function and Regulation of microRNA171 in Plant Stem Cell Homeostasis and Developmental Programing

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(5), С. 2544 - 2544

Опубликована: Фев. 25, 2022

MicroRNA171 (miR171), a group of 21-nucleotide single-strand small RNAs, is one ancient and conserved microRNA family in land plants. This review focuses on the recent progress understanding role miR171 plant stem cell homeostasis developmental patterning, regulation by cues environmental signals. Specifically, regulates shoot meristem activity phase transition through repressing

Язык: Английский

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Genome-Wide Identification and Expression Pattern of the GRAS Gene Family in Pitaya (Selenicereus undatus L.)

Biology, Год журнала: 2022, Номер 12(1), С. 11 - 11

Опубликована: Дек. 21, 2022

The GRAS gene family is one of the most important families transcriptional factors that have diverse functions in plant growth and developmental processes including axillary meristem patterning, signal-transduction, cell maintenance, phytohormone light signaling. Despite their importance, function genes pitaya fruit (Selenicereus undatus L.) remains unknown. Here, 45 members HuGRAS were identified genome, which was distributed on 11 chromosomes. All grouped into nine subfamilies using phylogenetic analysis with six other species: maize, rice, soybeans, tomatoes, Medicago truncatula Arabidopsis. Among genes, 12 selected from RNA-Seq data due to higher expression different tissues pitaya. In order verify data, these subjected for qRT-PCR validation. Nine exhibited relative plant. These categorized inlcuding DELLA (HuGRAS-1), SCL-3 (HuGRAS-7), PAT1 (HuGRAS-34, HuGRAS-35, HuGRAS-41), HAM (HuGRAS-37), SCR (HuGRAS-12) LISCL (HuGRAS-18, HuGRAS-25) might regulate development results present study provide valuable information improve tropical through a molecular conventional breeding program.

Язык: Английский

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Maintenance of stem cell activity in plant development and stress responses

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Дек. 12, 2023

Stem cells residing in plant apical meristems play an important role during postembryonic development. These stem are the wellspring from which tissues and organs of emerge. The shoot meristem (SAM) governs aboveground portions a plant, while root (RAM) orchestrates subterranean system. In their sessile existence, plants inextricably bound to environment must adapt various abiotic stresses, including osmotic stress, drought, temperature fluctuations, salinity, ultraviolet radiation, exposure heavy metal ions. environmental challenges exert profound effects on cells, potentially causing severe DNA damage disrupting equilibrium reactive oxygen species (ROS) Ca 2+ signaling these vital jeopardizing integrity survival. response challenges, have evolved mechanisms ensure preservation, restoration, adaptation meristematic cell niche. This enduring allows thrive habitats over extended periods. Here, we presented comprehensive overview cellular molecular intricacies surrounding initiation maintenance We also delved into employed by withstand respond stressors.

Язык: Английский

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The cellular basis of meristem development in fern gametophytes

Biochemical Society Transactions, Год журнала: 2025, Номер 53(01)

Опубликована: Фев. 13, 2025

The life cycle of land plants is characterized by alternating generations sexual gametophytes and asexual sporophytes. Unlike seed plants, seed-free vascular including ferns, initiate maintain pluripotent meristems during their gametophyte phase to sustain body expansion drive the formation organs for reproduction. This review summarizes meristem development among various fern species phase, focusing on cellular basis initiation, proliferation, termination. We different types gametophytic in apical cell (AC)-based meristems, multicellular marginal meristems. highlight both conserved lineage-specific patterns division, which are closely associated with these identities play crucial roles shaping morphology. Additionally, we recent progress understanding dynamics division growth that development, through studies integrate confocal live imaging computational quantitative analysis. Furthermore, discuss influence environmental genetic factors activity gametophytes, transcriptional regulators indeterminacy proliferation model Ceratopteris richardii.

Язык: Английский

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Genome-wide identification and expression analysis of the GRAS family under low-temperature stress in bananas

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Авг. 30, 2023

GRAS, named after GAI, RGA, and SCR, is a class of plant-specific transcription factors family that plays crucial role in growth development, signal transduction, various stress responses. To understand the biological functions banana GRAS gene family, genome-wide identification bioinformatics analysis was performed based on information from M. acuminata, balbisiana, itinerans genomic databases. In present study, we identified 73 MaGRAS, 59 MbGRAS, 58 MiGRAS genes bananas at whole-genome scale, 56 homologous were three genomes. Banana GRASs can be classified into 10 subfamilies, their structures revealed most gDNAs lack introns. The promoter sequences had large number cis-acting elements related to plant phytohormone, adversity responsiveness. expression pattern seven key members MaGRAS response low-temperature different tissues also examined by quantitative reverse polymerase chain reaction (qRT-PCR). microRNAs-MaGRASs target prediction showed perfect complementarity with five mac-miRNAs. all lowest roots, highest leaves during stress. MaSCL27-2, MaSCL27-3, MaSCL6-1 significantly lower under compared control, except for which slightly higher than 28°C control 4 h. dropped levels 24 h, 12 respectively. MaSCL27-4 MaSCL6-2 intermittently upregulated, rising 24h, while MaSCL22 less variable, remaining level small changes. summary, it tentatively hypothesized has an important function bananas. This study provides theoretical basis further analyzing resistance cold temperatures.

Язык: Английский

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Overexpression of Larch SCL6 Inhibits Transitions from Vegetative Meristem to Inflorescence and Flower Meristem in Arabidopsis thaliana (L.) Heynh.

Plants, Год журнала: 2024, Номер 13(9), С. 1232 - 1232

Опубликована: Апрель 29, 2024

SCARECROW-LIKE6 (SCL6) plays a role in the formation and maintenance of meristem. In Larix kaempferi (Lamb.) Carr., an important afforestation tree species China, SCL6 (LaSCL6) has two alternative splicing variants—LaSCL6-var1 LaSCL6-var2—which are regulated by microRNA171. However, their roles still unclear. this study, LaSCL6-var1 LaSCL6-var2 were transformed into Arabidopsis thaliana (L.) Heynh. genome, phenotypic characteristics transgenic A. thaliana, including germination percentage, root length, bolting time, flower silique times, inflorescence axis branch numbers, analyzed to reveal functions. It was found that overexpression shortened length 41% 31%, respectively, increased length. Compared with wild type, time plants delayed approximately 2–3 days, first times 3–4 last about 5 days. Overall, life cycle prolonged These results show LaSCL6 inhibited transitions from vegetative meristem arrest revealing fate transition

Язык: Английский

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A conserved GRAS-domain transcriptional regulator links meristem indeterminacy to sex determination in Ceratopteris gametophytes

Current Biology, Год журнала: 2024, Номер 34(15), С. 3454 - 3472.e7

Опубликована: Июль 25, 2024

Язык: Английский

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