Genetic dissection of MutL complexes in Arabidopsis meiosis
Nucleic Acids Research,
Год журнала:
2025,
Номер
53(5)
Опубликована: Фев. 27, 2025
Abstract
During
meiosis,
homologous
chromosomes
exchange
genetic
material
through
crossing
over.
The
main
crossover
pathway
relies
on
ZMM
proteins,
including
ZIP4
and
HEI10,
is
typically
resolved
by
the
MLH1/MLH3
heterodimer,
MutLγ.
Our
analysis
shows
that
while
MUS81
may
partially
compensate
for
MutLγ
loss,
its
role
remains
uncertain.
However,
our
multiple
mutant
unlikely
to
be
sole
resolvase
of
ZMM-protected
recombination
intermediates
when
absent.
Comparing
genome-wide
maps
mlh1
mutants
with
ZMM-deficient
lines
varying
HEI10
levels
reveals
interference
persists
in
but
weakened.
significant
reduction
also
increases
aneuploidy
offspring.
loss
can
suppressed
eliminating
FANCM
helicase.
Combined
lower-than-expected
chiasma
frequency,
this
suggests
absence,
some
are
ultimately
DNA
helicases
and/or
their
complexes
Top3α.
Elevated
MLH1
or
MLH3
expression
moderately
misregulation
drastically
reduces
numbers
plant
fertility,
highlighting
importance
tight
control
levels.
By
contrast,
PMS1,
a
component
MutLα
endonuclease,
appears
uninvolved
Together,
these
findings
demonstrate
unique
ZMM-dependent
regulation.
Язык: Английский
Breakpoint–chiasma interference in pericentric inversion heterokaryotypes
Genetics,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 6, 2025
Abstract
Heterozygous
inversion
breakpoints
inhibit
the
formation
of
chiasmata
in
their
vicinity,
and
it
has
been
suggested
that
they
do
so
through
same
mechanism
also
causes
interference
between
chiasmata.
In
this
paper,
I
therefore
extend
my
earlier
model
chiasma
to
account
for
pericentric
heterokaryotypes.
Using
analyze
recombination
sterility
datasets
Drosophila
melanogaster,
find
support
hypothesis
interfere
with
way
degree
other
do.
breakpoints,
like
chiasmata,
appear
show
negative
pericentromeric
region,
positive
elsewhere.
discuss
implications
these
findings
light
recent
HEI10
coarsening
hypothesis,
conclude
some
remarks
about
evolutionary
origin
interference.
Язык: Английский
Meiosis in plants: from Understanding to Manipulation
New Crops,
Год журнала:
2024,
Номер
2, С. 100055 - 100055
Опубликована: Окт. 12, 2024
Язык: Английский
The plant early recombinosome: a high security complex to break DNA during meiosis
Plant Reproduction,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 27, 2024
Язык: Английский
Kinesin‐1‐like protein PSS1 is essential for full‐length homologous pairing and synapsis in rice meiosis
The Plant Journal,
Год журнала:
2024,
Номер
120(3), С. 928 - 940
Опубликована: Сен. 16, 2024
SUMMARY
The
pairing
and
synapsis
of
homologous
chromosomes
are
crucial
for
their
correct
segregation
during
meiosis.
LINC
(Linker
Nucleoskeleton
Cytoskeleton)
complex
can
recruit
kinesin
protein
at
the
nuclear
envelope,
affecting
telomere
bouquet
formation
pairing.
Kinesin‐1‐like
Pollen
Semi‐Sterility1
(PSS1)
plays
a
pivotal
role
in
male
meiotic
chromosomal
behavior
is
essential
fertility
rice.
However,
its
exact
meiosis,
especially
as
involved
synapsis,
has
not
been
fully
elucidated.
Here,
we
generated
three
pss1
mutants
by
genome
editing
technology
to
dissect
PSS1
biological
functions
exhibit
alterations
radial
microtubule
organization
pachytene
manifest
deficiency
clustering,
which
critical
full‐length
We
reveal
that
serves
key
mediator
between
cytoskeleton,
thereby
regulating
transmitting
force
nuclei
facilitate
chromosome
Язык: Английский