Kinesin‐1‐like protein PSS1 is essential for full‐length homologous pairing and synapsis in rice meiosis DOI
Yue Zhou, Yafei Li, Hanli You

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 120(3), P. 928 - 940

Published: Sept. 16, 2024

SUMMARY The pairing and synapsis of homologous chromosomes are crucial for their correct segregation during meiosis. LINC (Linker Nucleoskeleton Cytoskeleton) complex can recruit kinesin protein at the nuclear envelope, affecting telomere bouquet formation pairing. Kinesin‐1‐like Pollen Semi‐Sterility1 (PSS1) plays a pivotal role in male meiotic chromosomal behavior is essential fertility rice. However, its exact meiosis, especially as involved synapsis, has not been fully elucidated. Here, we generated three pss1 mutants by genome editing technology to dissect PSS1 biological functions exhibit alterations radial microtubule organization pachytene manifest deficiency clustering, which critical full‐length We reveal that serves key mediator between cytoskeleton, thereby regulating transmitting force nuclei facilitate chromosome

Language: Английский

Genetic dissection of MutL complexes in Arabidopsis meiosis DOI Creative Commons
Nadia Kbiri, Nadia Fernández‐Jiménez, Wojciech Dzięgielewski

et al.

Nucleic Acids Research, Journal Year: 2025, Volume and Issue: 53(5)

Published: Feb. 27, 2025

Abstract During meiosis, homologous chromosomes exchange genetic material through crossing over. The main crossover pathway relies on ZMM proteins, including ZIP4 and HEI10, is typically resolved by the MLH1/MLH3 heterodimer, MutLγ. Our analysis shows that while MUS81 may partially compensate for MutLγ loss, its role remains uncertain. However, our multiple mutant unlikely to be sole resolvase of ZMM-protected recombination intermediates when absent. Comparing genome-wide maps mlh1 mutants with ZMM-deficient lines varying HEI10 levels reveals interference persists in but weakened. significant reduction also increases aneuploidy offspring. loss can suppressed eliminating FANCM helicase. Combined lower-than-expected chiasma frequency, this suggests absence, some are ultimately DNA helicases and/or their complexes Top3α. Elevated MLH1 or MLH3 expression moderately misregulation drastically reduces numbers plant fertility, highlighting importance tight control levels. By contrast, PMS1, a component MutLα endonuclease, appears uninvolved Together, these findings demonstrate unique ZMM-dependent regulation.

Language: Английский

Citations

0

Breakpoint–chiasma interference in pericentric inversion heterokaryotypes DOI
Øystein Kapperud

Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Abstract Heterozygous inversion breakpoints inhibit the formation of chiasmata in their vicinity, and it has been suggested that they do so through same mechanism also causes interference between chiasmata. In this paper, I therefore extend my earlier model chiasma to account for pericentric heterokaryotypes. Using analyze recombination sterility datasets Drosophila melanogaster, find support hypothesis interfere with way degree other do. breakpoints, like chiasmata, appear show negative pericentromeric region, positive elsewhere. discuss implications these findings light recent HEI10 coarsening hypothesis, conclude some remarks about evolutionary origin interference.

Language: Английский

Citations

0

Meiosis in plants: from Understanding to Manipulation DOI Creative Commons
Like Chen, Kejian Wang, Chun Wang

et al.

New Crops, Journal Year: 2024, Volume and Issue: 2, P. 100055 - 100055

Published: Oct. 12, 2024

Language: Английский

Citations

2

The plant early recombinosome: a high security complex to break DNA during meiosis DOI Creative Commons
Nahid Rafiei, Arnaud Ronceret

Plant Reproduction, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Language: Английский

Citations

1

Kinesin‐1‐like protein PSS1 is essential for full‐length homologous pairing and synapsis in rice meiosis DOI
Yue Zhou, Yafei Li, Hanli You

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 120(3), P. 928 - 940

Published: Sept. 16, 2024

SUMMARY The pairing and synapsis of homologous chromosomes are crucial for their correct segregation during meiosis. LINC (Linker Nucleoskeleton Cytoskeleton) complex can recruit kinesin protein at the nuclear envelope, affecting telomere bouquet formation pairing. Kinesin‐1‐like Pollen Semi‐Sterility1 (PSS1) plays a pivotal role in male meiotic chromosomal behavior is essential fertility rice. However, its exact meiosis, especially as involved synapsis, has not been fully elucidated. Here, we generated three pss1 mutants by genome editing technology to dissect PSS1 biological functions exhibit alterations radial microtubule organization pachytene manifest deficiency clustering, which critical full‐length We reveal that serves key mediator between cytoskeleton, thereby regulating transmitting force nuclei facilitate chromosome

Language: Английский

Citations

0