Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119551 - 119551
Опубликована: Фев. 1, 2025
Язык: Английский
Phytomedicine, Год журнала: 2024, Номер 128, С. 155502 - 155502
Опубликована: Март 11, 2024
Язык: Английский
Процитировано
6
International Journal of Surgery, Год журнала: 2024, Номер unknown
Опубликована: Март 28, 2024
Introduction: Septic cardiomyopathy is a sepsis-mediated cardiovascular complication with severe microcirculatory malperfusion. Emerging evidence has highlighted the protective effects of pulsatile flow in case disturbance, yet underlying mechanisms are still elusive. The objective this study was to investigate N6-methyladenosine (m 6 A) modification alleviation septic associated extracorporeal membrane oxygenation (ECMO)-generated flow. Methods: Rat model established and supported under ECMO either or non-pulsatile Peripheral perfusion index (PPI) cardiac function parameters were measured using ultrasonography. Dot blot assay applied examine m A level, while qRT-PCR, Western blot, immunofluorescence, immunohistochemistry used measure expressions related genes. RNA immunoprecipitation performed validate interaction between molecules. Results: ECMO-generated significantly elevates PPI, improves myocardial function, protects endothelium, prolongs survival rat models cardiomyopathy. mediates METTL14-mediated zonula occludens- (ZO-) 1 mRNA which stabilizes ZO-1 depending on presence YTHDF2. suppresses PI3K-Akt signaling pathway, downstream molecule Foxo1, negative transcription factor METTL14, binds METTL14 promoter inhibits METTL14-induced modification. Conclusion: increases attenuates progression cardiomyopathy, suggesting that pulsatility might be new therapeutic strategy by alleviating disturbance.
Язык: Английский
Процитировано
6
Archives of Toxicology, Год журнала: 2024, Номер 98(9), С. 2907 - 2918
Опубликована: Май 29, 2024
Язык: Английский
Процитировано
5
Chinese Medical Journal, Год журнала: 2024, Номер unknown
Опубликована: Июнь 14, 2024
Abstract Background: G protein-coupled receptor kinase 2 (GRK2) could participate in the regulation of diverse cells via interacting with non-G-protein-coupled receptors. In present work, we explored how paroxetine, a GRK2 inhibitor, modulates differentiation and activation immune rheumatoid arthritis (RA). Methods: The blood samples healthy individuals RA patients were collected between July 2021 March 2022 from First Affiliated Hospital Anhui Medical University. C57BL/6 mice used to induce collagen-induced (CIA) model. Flow cytometry analysis was characterize function dendritic (DCs)/T cells. Co-immunoprecipitation explore specific molecular mechanism. Results: RA, high expression peripheral lymphocytes, accompanied by increases phosphatidylinositol 3 (PI3K), protein B (AKT), mammalian target rapamycin (mTOR). animal model, decrease regulatory T (T regs ), an increase cluster 8 positive (CD8 + ) cells, maturation DCs observed. Paroxetine, when vitro CIA mice, restrained CD8 induced proportion . Paroxetine inhibited secretion pro-inflammatory cytokines, C-C motif chemokine 7 Simultaneously, paroxetine upregulated programmed death ligand 1, anti-inflammatory cytokines. Additionally, PI3K–AKT–mTOR metabolic pathway both This associated reduction mitochondrial membrane potential changes utilization glucose lipids, particularly DCs. reversed PI3K–AKT 740 Y-P (a PI3K agonist) through inhibiting interaction Conclusion: exerts immunosuppressive effect targeting GRK2, which subsequently inhibits metabolism-related cell RA.
Язык: Английский
Процитировано
5
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2023, Номер 1869(4), С. 166656 - 166656
Опубликована: Янв. 25, 2023
Язык: Английский
Процитировано
11
Frontiers in Immunology, Год журнала: 2023, Номер 14
Опубликована: Фев. 13, 2023
There is a complex interaction between chronic kidney disease (CKD) and ulcerative colitis (UC), but the pathophysiological mechanisms underlying coexistence of CKD UC are unclear. This study aimed to investigate key molecules pathways that may mediate co-occurrence through quantitative bioinformatics analysis based on public RNA-sequencing database. The discovery datasets (GSE66494) (GSE4183), as well validation (GSE115857) (GSE10616), were downloaded from Gene Expression Omnibus (GEO) After identifying differentially expressed genes (DEGs) with GEO2R online tool, Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses for DEGs performed. Next, protein-protein network was constructed Search Tool Retrieval Interacting (STRING) visualized by Cytoscape. modules identified plug-in MCODE hub screened using CytoHubba. Then, correlation immune cell infiltration analyzed, receiver operating characteristic curves used assess predictive value genes. Finally, immunostaining human specimens validate relevant findings. A total 462 common selected further analyses. GO KEGG indicated these primarily enriched in immune- inflammation-related pathways. Among them, PI3K-Akt signaling ranked top both cohorts, signal molecule phosphorylated Akt (p-Akt) shown be significantly overexpressed kidneys colons, elevated CKD-UC comorbidity specimens. Moreover, nine candidate genes, including CXCL8, CCL2, CD44, ICAM1, IL1A, CXCR2, PTPRC, ITGAX, CSF3, identified, which ICAM1 validated gene. Besides, revealed neutrophils, macrophages, CD4+ T memory cells accumulated diseases, remarkably associated neutrophil infiltration. Furthermore, intercellular adhesion molecule1 (ICAM1)-mediated upregulated colon biopsies patients, increased patients diagnosed UC. had critical diagnostic marker Our elucidated response, pathway, ICAM1-mediated might pathogenesis UC, potential biomarker therapeutic target two diseases.
Язык: Английский
Процитировано
11
Journal of Ethnopharmacology, Год журнала: 2023, Номер 319, С. 117239 - 117239
Опубликована: Сен. 28, 2023
Язык: Английский
Процитировано
11
Journal of Ethnopharmacology, Год журнала: 2024, Номер 329, С. 118169 - 118169
Опубликована: Апрель 16, 2024
The Ba-Qi-Rougan formula (BQRGF) is a traditional and effective compound prescription from Traditional Chinese Medicine (TCM) utilized in treating hepatic fibrosis (HF).
Язык: Английский
Процитировано
4
Frontiers in Pharmacology, Год журнала: 2022, Номер 13
Опубликована: Окт. 4, 2022
Extensive research has implicated inflammation and oxidative stress in the development of multiple diseases, such as diabetes, hepatitis, arthritis. Kinsenoside (KD), a bioactive glycoside component extracted from medicinal plant Anoectochilus roxburghii , been shown to exhibit potent anti-inflammatory anti-oxidative abilities. In this review, we summarize effects KD, including hepatoprotection, pro-osteogenesis, anti-hyperglycemia, vascular protection, immune regulation, vision infection inhibition, which are partly responsible for suppressing signaling stress. The protective action KD against dysfunctional lipid metabolism is also associated with limiting inflammatory signals, due crosstalk between metabolism. Ferroptosis, process involved both damage, potentially regulated by KD. addition, discuss physicochemical properties pharmacokinetic profiles Advances cultivation artificial synthesis techniques promising evidence that shortage raw materials required production can be overcome. novel drug delivery systems improve vivo rapid clearance poor bioavailability integrated aim offer insights into molecular mechanisms underlying therapeutic role lay solid foundations utilization clinical practice.
Язык: Английский
Процитировано
16
Clinical Immunology, Год журнала: 2022, Номер 248, С. 109217 - 109217
Опубликована: Дек. 27, 2022
Язык: Английский
Процитировано
15