bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 28, 2023
ABSTRACT
Background
Sex,
as
a
critical
biological
variable,
has
historically
been
underappreciated,
despite
the
pervasive
influence
of
sexual
dimorphism
across
physiological
and
pathological
processes.
A
significant
obstacle
to
advancing
sex-biased
research
is
absence
an
effective
animal
model.
In
recent
years,
castration
emerged
potential
model
for
elucidating
sex-based
differences
in
context
healthy
aging,
where
it
shown
equalize
lifespan
growth
trajectories
genetically
diverse
mice.
However,
molecular
shifts
induced
by
common
laboratory
models,
such
C57BL/6
mice,
broader
applicability
this
other
sex-related
contexts
remain
largely
unexplored.
Methods
We
employed
multi-omics
observational
analyses
investigate
changes
associated
with
sex
hormones
following
castration.
analyzed
serum,
kidney,
liver
samples
from
12-week-old
18-month-old
castrated
male
alongside
intact
female
counterparts.
The
was
further
applied
assess
cisplatin-induced
toxicity
age-related
cognitive
decline
comparison
unaltered
controls.
Results
LC-MS/MS
metabolomics
revealed
that
males
exhibited
substantial
alterations
steroid
hormone
levels
increased
concentrations
antioxidant
compounds,
taurine,
identical
diets.
Integrated
metabolome-transcriptome
analysis
confirmed
distinct
patterns
lipid
peroxidation
oxidative
stress
sham-operated
female,
male,
Histopathological
evaluations
cisplatin
treatment
aging-related
behavioral
tests
demonstrated
model’s
utility
investigating
sex-dependent
drug
decline.
These
findings
underscored
role
modulating
both
defense
mechanisms
performance.
Conclusion
This
study
provides
systematic
spectrum
on
demonstrates
its
capacity
feminize
metabolic
transcriptomic
profiles,
establishing
valuable
tool
exploring
hormone-driven
differences.
Our
lay
groundwork
mechanistic
studies
broaden
applications
biomedical
domains.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Сен. 20, 2023
Abstract
Idiopathic
pulmonary
fibrosis
(IPF)
is
a
chronic
progressive
lung
disease
with
reduced
quality
of
life
and
earlier
mortality,
but
its
pathogenesis
key
genes
are
still
unclear.
In
this
investigation,
bioinformatics
was
used
to
deeply
analyze
the
IPF
related
genes,
so
as
investigate
potential
molecular
provide
guidance
for
clinical
treatment.
Next
generation
sequencing
dataset
GSE213001
obtained
from
Gene
Expression
Omnibus
(GEO),
differentially
expressed
(DEGs)
were
identified
between
normal
control
group.
The
DEGs
group
screened
DESeq2
package
R
language.
Ontology
(GO)
REACTOME
pathway
enrichment
analyses
performed..
Using
g:Profiler,
function
analysis
performed.
Then,
protein–protein
interaction
(PPI)
network
constructed
via
Integrated
Interactions
Database
(IID)
database.
Cytoscape
Network
Analyzer
identify
hub
genes.
miRNet
NetworkAnalyst
database
construct
targeted
microRNAs
(miRNAs)
transcription
factors
(TFs)
Finally
receiver
operating
characteristic
(ROC)
curve
validates
A
total
958
out
in
study,
including
479
up
regulated
down
Most
significantly
enriched
response
stimulus,
GPCR
ligand
binding,
microtubule-based
process
defective
GALNT3
causes
HFTC.
combination
results
PPI
network,
miRNA-hub
gene
regulatory
TF-hub
LRRK2,
BMI1,
EBP,
MNDA,
KBTBD7,
KRT15,
OTX1,
TEKT4,
SPAG8
EFHC2
selected.
Our
findings
will
contribute
identification
biomarkers
novel
strategies
treatment
IPF,
strategy
therapy.
Indian Journal of Critical Care Medicine,
Год журнала:
2024,
Номер
28(9), С. 879 - 886
Опубликована: Авг. 29, 2024
Septic
shock
is
associated
with
high
mortality
and
there
significant
heterogeneity
in
the
host
response.
The
aim
of
this
study
was
to
understand
genome-wide
expression
transcriptomic
signatures
children
septic
correlate
them
outcomes.
Abstract
Visual
exposure
to
dim,
green,
light
has
been
found
reduce
pain
levels
in
patients
living
with
migraine,
low
back
pain,
and
fibromyalgia.
Preclinical
studies
discovered
that
the
analgesic
effect
of
green
was
due
central
release
endogenous
opioids
a
reduction
inflammatory
cytokines
cerebrospinal
fluid.
The
present
study
assessed
therapy
(GLT)
on
joint
rat
model
osteoarthritis
(OA)
investigated
role
endolipids.
Male
female
Wistar
rats
(207-318
g)
received
an
intra-articular
injection
sodium
monoiodoacetate
(3
mg
50
μL
saline)
into
knee
induce
OA.
On
day
9,
animals
were
placed
room
illuminated
by
either
white
(neutral-white
4000K;
20
lux)
or
(wavelength:
525
nm;
luminance:
for
5
days
(8
hours
per
day).
Joint
nociception
von
Frey
hair
algesiometry,
dynamic
weight
bearing,
vivo
single
unit
extracellular
recordings
from
mechanonociceptors.
Compared
light,
GLT
significantly
reduced
secondary
mechanical
hypersensitivity
both
sexes
improved
hindlimb
bearing
females
only.
There
no
nociceptor
activity
sex.
Serum
lipidomics
indicated
increase
circulating
endolipids
response
GLT,
particularly
N
-acyl-glycines.
Partial
blockade
endocannabinoid
system
G
protein
receptor-18/cannabinoid-1
receptor
antagonist
AM281
(500
μg/kg
i.p.)
attenuated
GLT-induced
analgesia.
These
data
show
first
time
acts
OA
upregulating
endolipids,
which
then
engage
system.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 28, 2023
ABSTRACT
Background
Sex,
as
a
critical
biological
variable,
has
historically
been
underappreciated,
despite
the
pervasive
influence
of
sexual
dimorphism
across
physiological
and
pathological
processes.
A
significant
obstacle
to
advancing
sex-biased
research
is
absence
an
effective
animal
model.
In
recent
years,
castration
emerged
potential
model
for
elucidating
sex-based
differences
in
context
healthy
aging,
where
it
shown
equalize
lifespan
growth
trajectories
genetically
diverse
mice.
However,
molecular
shifts
induced
by
common
laboratory
models,
such
C57BL/6
mice,
broader
applicability
this
other
sex-related
contexts
remain
largely
unexplored.
Methods
We
employed
multi-omics
observational
analyses
investigate
changes
associated
with
sex
hormones
following
castration.
analyzed
serum,
kidney,
liver
samples
from
12-week-old
18-month-old
castrated
male
alongside
intact
female
counterparts.
The
was
further
applied
assess
cisplatin-induced
toxicity
age-related
cognitive
decline
comparison
unaltered
controls.
Results
LC-MS/MS
metabolomics
revealed
that
males
exhibited
substantial
alterations
steroid
hormone
levels
increased
concentrations
antioxidant
compounds,
taurine,
identical
diets.
Integrated
metabolome-transcriptome
analysis
confirmed
distinct
patterns
lipid
peroxidation
oxidative
stress
sham-operated
female,
male,
Histopathological
evaluations
cisplatin
treatment
aging-related
behavioral
tests
demonstrated
model’s
utility
investigating
sex-dependent
drug
decline.
These
findings
underscored
role
modulating
both
defense
mechanisms
performance.
Conclusion
This
study
provides
systematic
spectrum
on
demonstrates
its
capacity
feminize
metabolic
transcriptomic
profiles,
establishing
valuable
tool
exploring
hormone-driven
differences.
Our
lay
groundwork
mechanistic
studies
broaden
applications
biomedical
domains.
