Preparation and Tumor Inhibitory Activity of Tricin from Carex Meyeriana Kunth DOI Creative Commons

Baiji Cui,

Jie Sun,

Sheng Chang

и другие.

Molecules, Год журнала: 2024, Номер 29(19), С. 4530 - 4530

Опубликована: Сен. 24, 2024

This study describes the purification and preparation of tricin (5, 7, 4-trihydroxy-3, 5-dimethoxyflavone) from Carex Meyeriana Kunth via adsorption desorption using macroporous resins high-performance liquid chromatography. Six were tested to evaluate static capacities. The HPD-300 resin was selected as material enrich because its suitable Adsorption thermodynamics kinetics studied on resin, results agreed with Langmuir model quasi-second-order model, respectively. parameters dynamic tests then optimized. purity increased 2.6 mg/g 45.1 a recovery yield 76.4% after resin. Then, Prep-HPLC used further purify tricin. reached 99.4%, 78.0% thereafter. Tricin exerts an inhibitory effect proliferation various tumor cells, including gastric cancer SGC-7901 cells. It significantly suppresses cell colony formation while also altering cycle progression metabolism by decreasing proportion cells in G0/G1 phase increasing S G2/M phases. Additionally, affects efficiency lactate production, ATP content, glucose uptake. These findings suggest that may impede through impact energy metabolism.

Язык: Английский

Programmed cell death: molecular mechanisms, biological functions, diseases, and therapeutic targets DOI Creative Commons

Shen’er Qian,

Yao Long, Guolin Tan

и другие.

MedComm, Год журнала: 2024, Номер 5(12)

Опубликована: Ноя. 28, 2024

Programmed cell death represents a precisely regulated and active cellular demise, governed by complex network of specific genes proteins. The identification multiple forms programmed has significantly advanced the understanding its intricate mechanisms, as demonstrated in recent studies. A thorough grasp these processes is essential across various biological disciplines study diseases. Nonetheless, despite notable progress, exploration relationship between disease, well clinical application, are still nascent stage. Therefore, further development corresponding therapeutic methods strategies holds substantial potential. Our review provides detailed examination primary mechanisms behind apoptosis, autophagy, necroptosis, pyroptosis, ferroptosis. Following this, discussion delves into functions diseases associated dysregulated death. Finally, we highlight existing potential targets focused on cancers neurodegenerative This aims to summarize latest insights from more reliable approach for transformation.

Язык: Английский

Процитировано

4
AMPK signaling pathway as a potential therapeutic target for Parkinson's disease DOI Creative Commons
Seyed Zanyar Athari, Fereshteh Farajdokht, ‬Rana Keyhanmanesh

и другие.

Advanced Pharmaceutical Bulletin, Год журнала: 2023, Номер unknown

Опубликована: Окт. 14, 2023

Parkinson's disease (PD) is the second most common neurodegenerative caused by loss of dopaminergic neurons. Genetic factors, inflammatory responses, oxidative stress, metabolic disorders, cytotoxic and mitochondrial dysfunction are all involved in neuronal death diseases. The risk PD can be higher aging individuals due to decreased function, energy metabolism, AMP-activated protein kinase (AMPK) function. potential AMPK regulate disorders lies its ability enhance antioxidant capacity, reduce improve decrease mitophagy macroautophagy, inhibit inflammation. In addition, it has been shown that modulating catalytic activity protect nervous system. This article reviews mechanisms which activation modulate PD.

Язык: Английский

Процитировано

9
Elucidating the Role of Trem2 in Lipid Metabolism and Neuroinflammation DOI Creative Commons

Chenhui Zhao,

Wei Qi,

Xiaoping Lv

и другие.

CNS Neuroscience & Therapeutics, Год журнала: 2025, Номер 31(4)

Опубликована: Апрель 1, 2025

ABSTRACT Background Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive impairment and neuroinflammation. Astrocytes play key role in the neuroinflammatory environment of AD, especially through lipid metabolism regulation. However, mechanisms which astrocytes, particularly triggering receptor expressed on myeloid cells 2 (Trem2) receptor, contribute to dysregulation neuroinflammation AD remain inadequately understood. Methods We employed an mouse model integrated single‐cell RNA sequencing (scRNA‐seq), transcriptomics, high‐throughput metabolomics analyze inflammatory profiles astrocytes. Differential gene expression was further validated with GEO database, vitro vivo experiments were conducted assess impact Trem2 modulation astrocytic inflammation composition. Results Our findings demonstrate that modulates affecting fatty acid phospholipid pathways. In model, suppressed, enhancing nuclear factor‐κB (NF‐κB) signaling promoting secretion pro‐inflammatory factors such as tumor necrosis factor‐α (TNF‐α) interleukin‐6 (IL‐6). overexpression reduced altered composition, attenuating both vivo. These results underscore Trem2's regulatory its significant AD. Conclusions This study identifies pivotal regulator providing potential molecular targets for early intervention therapeutic strategies aimed at mitigating progression.

Язык: Английский

Процитировано

0
Tricin Delays Aging and Enhances Muscle Function via Activating AMPK-Mediated Autophagy in Diverse Model Organisms DOI
Yunfei Zhu,

Xing‐Yue Zhou,

Cai Lan

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Aging leads to progressive decline in the functions of cells, tissues, and organs, severely affecting muscle performance overall health, highlighting urgent need for effective therapeutic agents. This study investigated antiaging properties tricin, a flavonoid abundant grains, using biological models, including human fibroblasts, Caenorhabditis elegans (C. elegans), mice. Tricin significantly alleviated senescent phenotype fibroblasts induced by D-galactose (D-gal), doxorubicin, replicative senescence, as evidenced reduced SA-β-gal activity, downregulated senescence markers (p16, p21), decreased SASP factors. Mechanistically, tricin binds AMPK activates AMPK-mTOR-p70S6K signaling pathway, promoting autophagy delaying cellular aging. In vivo, extended lifespan, enhanced stress resistance, improved mobility C. through aak-2/AMPK-mediated autophagy. D-gal-induced aging mice, function, reducing p16, p21, expression tissues. These findings underscore tricin's potential promising via AMPK-mediated activation.

Язык: Английский

Процитировано

0
Nucleolin inhibits α-synuclein to attenuate aconitine’s neurotoxicity DOI
Guangxin Liu, Wenqiang Zuo, Zhen Zhang

и другие.

Phytomedicine, Год журнала: 2025, Номер 144, С. 156932 - 156932

Опубликована: Июнь 2, 2025

Язык: Английский

Процитировано

0
In vitro modulation of mTOR and mGlur5 influence α-synuclein accumulation DOI Creative Commons

Viktoria Xing,

Kyle K. Biggar, Stephen S. G. Ferguson

и другие.

Molecular Brain, Год журнала: 2024, Номер 17(1)

Опубликована: Фев. 15, 2024

Abstract One of the main hallmarks Parkinson’s disease (PD) is abnormal alpha-synuclein (α-syn) aggregation which forms component intracellular Lewy body inclusions. This short report used preformed α-syn fibrils, as well an A53T mutant adenovirus to mimic conditions pathological protein in dopaminergic human derived SH-SY5Y neural cells. Since there evidence that mTOR pathway and glutamatergic signaling each influence aggregation, we also assessed impact inhibitor, rapamycin mGluR5 allosteric modulator, CTEP. We found both CTEP induced a significant reduction fibrils cells this effect was associated with enhancement autophagic factors. These data support possibility (or rapamycin) might be useful pharmacological approach target accumulation by promoting degradation or enhanced clearance.

Язык: Английский

Процитировано

2
Effect of Tricin on cardiomyocyte damage caused by diabetic cardiomyopathy (DCM) DOI Creative Commons
Rong Yu, Yaping Zhang, Tong Wang

и другие.

BMC Cardiovascular Disorders, Год журнала: 2024, Номер 24(1)

Опубликована: Ноя. 23, 2024

Flavonoid compounds exhibit remarkable antioxidant and anti-inflammatory properties in DCM various other diseases. However, the specific mechanisms by which Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exerts its effects context of remain to be elucidated. Rat H9C2 cells were cultured subjected high glucose conditions establish a cell model. Tricin was administered varying concentrations evaluate on cellular oxidative stress markers, including ROS, LDH, SOD. Additionally, levels inflammatory cytokines TNF-α, IL-1β, IL-6, as well expression TLR4, MYD88, p-NF-κB, assessed through ELISA Western blotting. treatment significantly ameliorated glucose-induced cells, evidenced reduced ROS LDH increased SOD dose-dependent manner. Furthermore, effectively suppressed elevation pro-inflammatory IL-6. also inhibited overactivation TLR4-MYD88-NF-κB signaling pathway, suggesting role modulating key processes DCM. exhibits protective against cardiac damage By reducing inflammation, inhibiting shows significant therapeutic potential for treatment. This study underscores value novel approach managing diabetic cardiomyopathy, warranting further research clinical investigation. Not applicable.

Язык: Английский

Процитировано

2
The potential therapeutic strategy in combating neurodegenerative diseases: Focusing on natural products DOI
Li Gao, Ximing J. Yang, Yixiao Dong

и другие.

Pharmacology & Therapeutics, Год журнала: 2024, Номер 264, С. 108751 - 108751

Опубликована: Ноя. 9, 2024

Язык: Английский

Процитировано

1
Exploration of the Functional Constituents of the Substrate of Flammulina velutipes DOI Creative Commons
Ruixiang Jiang,

Renqing Zheng,

Bin Yuan

и другие.

Separations, Год журнала: 2024, Номер 11(11), С. 311 - 311

Опубликована: Окт. 28, 2024

After harvesting, the substrate of Flammulina velutipes (SFV) is repurposed for uses such as feed, fuel, and culture medium. This study identified four phenolic acids one flavonoid from SFV first time using solvent extraction chromatography: ferulic acid (1), ρ-coumaric (2), vanillic (3), 3-methoxygallic (4), tricin (5). They showed significant activity in DPPH scavenging test with concentrations determined by HPLC follows: (218.88 mg/kg), (157.31 (281.54 (33.85 (713.42 mg/kg). These findings indicate that a valuable source bioactive compounds medicinal health-promoting applications.

Язык: Английский

Процитировано

0
Preparation and Tumor Inhibitory Activity of Tricin from Carex Meyeriana Kunth DOI Creative Commons

Baiji Cui,

Jie Sun,

Sheng Chang

и другие.

Molecules, Год журнала: 2024, Номер 29(19), С. 4530 - 4530

Опубликована: Сен. 24, 2024

This study describes the purification and preparation of tricin (5, 7, 4-trihydroxy-3, 5-dimethoxyflavone) from Carex Meyeriana Kunth via adsorption desorption using macroporous resins high-performance liquid chromatography. Six were tested to evaluate static capacities. The HPD-300 resin was selected as material enrich because its suitable Adsorption thermodynamics kinetics studied on resin, results agreed with Langmuir model quasi-second-order model, respectively. parameters dynamic tests then optimized. purity increased 2.6 mg/g 45.1 a recovery yield 76.4% after resin. Then, Prep-HPLC used further purify tricin. reached 99.4%, 78.0% thereafter. Tricin exerts an inhibitory effect proliferation various tumor cells, including gastric cancer SGC-7901 cells. It significantly suppresses cell colony formation while also altering cycle progression metabolism by decreasing proportion cells in G0/G1 phase increasing S G2/M phases. Additionally, affects efficiency lactate production, ATP content, glucose uptake. These findings suggest that may impede through impact energy metabolism.

Язык: Английский

Процитировано

0