Ghrelin Alleviates Inflammation, Insulin Resistance, and Reproductive Abnormalities in Mice with Polycystic Ovary Syndrome via the TLR4-NF-κB Signaling Pathway

Discovery Medicine, Год журнала: 2024, Номер 36(184), С. 946 - 946

Опубликована: Янв. 1, 2024

Polycystic ovary syndrome (PCOS) commonly impacts fertile females with potentially severe effects on fertility and metabolism. Blood ghrelin levels are lower in PCOS patients, exogenous supplements have been proposed for their potential to trigger anti-inflammatory at the cellular level. This study aimed investigate whether pretreatment reduced inflammation, insulin resistance, reproductive abnormalities underlying mechanism of this disorder.

Язык: Английский

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Ampelopsis japonica aqueous extract improves ovulatory dysfunction in PCOS by modulating lipid metabolism

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 170, С. 116093 - 116093

Опубликована: Дек. 29, 2023

Polycystic ovary syndrome (PCOS) is a highly prevalent endocrine and metabolic disorder that closely associated with the proliferation apoptosis of ovarian granulosa cells (GCs). Ampelopsis japonica (AJ) dried tuberous root (Thunb.) Makino (A. japonica), anti-inflammatory, antioxidant, antibacterial, antiviral, wound-healing, antitumor properties; however, it unclear whether this herb has therapeutic effect on PCOS. Therefore, study aimed to investigate pharmacological AJ PCOS reveal its potential mechanism action. A rat model was established using letrozole. After establishing model, rats received oral treatment Diane-35 (Positive drug: ethinylestradiol + cyproterone tablets) for 2 weeks. Lipidomics conducted liquid-phase mass spectrometry chromatography. significantly regulated serum hormone levels attenuated pathological variants in ovaries Furthermore, reduced apoptotic rate GCs. Lipidomic analysis revealed modulated glycerolipid glycerophospholipid pathways mediated by lipoprotein lipase (Lpl), diacylglycerol choline phosphotransferase (Chpt1), choline/ethanolamine (Cept1). we may reduce GC modulating lipid metabolism, ultimately improving ovulatory dysfunction novel candidate treatment.

Язык: Английский

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Transcriptomic Signatures of WNT-Driven Pathways and Granulosa Cell-Oocyte Interactions during Primordial Follicle Activation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 11, 2024

Abstract Primordial follicle activation (PFA) is a pivotal event in female reproductive biology, coordinating the transition from quiescent to growing follicles. This study employed comprehensive single-cell RNA sequencing gain insights into detailed regulatory mechanisms governing synchronized dormancy and between granulosa cells (GCs) oocytes with progression of PFA process. Wntless ( Wls ) conditional knockout (cKO) mice served as unique model, suppressing pre-GCs GCs, disrupting somatic cell-derived WNT signaling ovary. Our data revealed immediate transcriptomic changes GCs post-PFA cKO mice, leading divergent trajectory, while exhibited modest alterations. Subpopulation analysis identified molecular pathways affected by on GC maturation, along specific gene signatures linked dormant activated oocytes. Despite minimal evidence continuous up-regulation dormancy-related genes oocytes, loss (pre-)GCs impacted expression even before PFA, subsequently influencing them globally. The infertility observed was attributed compromised GC-oocyte crosstalk microenvironment for highlights role WNT-signaling pathway its signature, emphasizing importance intercellular orchestrating folliculogenesis. Author summary In mammalian ovaries, primordial follicles protect wasteful consumption. We explored complex dynamics critical process that underlies health, focus signaling. By utilizing mouse model disrupted signaling, our reveals novel during PFA. Single-cell transcriptome profiling uncovered distinct cell populations highlighted impact ovarian types. post-PFA, which affects their differentiation trajectories establishing function. further examined consequences microenvironments interactions, elucidated altered oocyte subclusters potential links infertility. These findings provide foundation understanding connections contributes valuable knowledge clinical relevance fertility.

Язык: Английский

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The impact of Lactococcus lactis KUST48 on the transcription profile of Aeromonas hydrophila -infected zebrafish spleen

Microbiology Spectrum, Год журнала: 2024, Номер 12(4)

Опубликована: Март 5, 2024

, an aquatic pathogenic bacterium, has been found to infect many fish species and cause huge aquaculture losses. Antibiotics are the most common drugs used treat these infections. However, antibiotic abuse can lead development of resistance. Probiotics have potential replace antibiotics for preventing Zebrafish (

Язык: Английский

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Serum PM20D1 levels in patients with idiopathic pulmonary arterial hypertension and its clinical significance

BMC Cardiovascular Disorders, Год журнала: 2024, Номер 24(1)

Опубликована: Апрель 13, 2024

Abstract Objective This study aimed to investigate the serum levels of Peptidase M20 domain containing 1 (PM20D1) in idiopathic pulmonary arterial hypertension (IPAH) patients and examine its association with lipid metabolism, echocardiography, hemodynamic parameters. Methods prospective observational research enrolled 103 IPAH from January 2018 2022. Enzyme-linked immunosorbent assay (ELISA) was used measure PM20D1 all before treatment within 24 h admission. Demographic data, parameters biomarkers were also collected. Results The deceased group had significantly elevated age, right atrial (RA), mean pressure (mPAP), (mRAP), capillary wedge (PCWP), vascular resistance (PVR) decreased 6 min walking distance (6MWD) tricuspid annulus peak systolic velocity (TASPV). showed significant decreases PM20D1, low-density lipoprotein cholesterol (LDL-C), albumin (ALB). Additionally, negatively correlated RA, NT-proBNP positively PVR, ALB, 6MWD, TAPSV. Moreover, has potential as a biomarker for predicting patients’ prognosis. Finally, logistic regression analysis indicated that NT-proBNP, TASPV, RA 6MWD identified risk factors mortality patients. Conclusion Our findings poor factor associated

Язык: Английский

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Flavonoid Supplementation Is Beneficial for Polycystic Ovary Syndrome: A Systematic Review and Meta-analysis

Nutrition Reviews, Год журнала: 2024, Номер unknown

Опубликована: Июль 22, 2024

Abstract Context Polycystic ovary syndrome (PCOS) is a prevalent hormonal imbalance that predominantly affects women in their reproductive years. Previous studies have yielded conflicting conclusions. Objective This an updated meta-analysis aiming to explore the connection between flavonoid supplementation and PCOS. Data Sources Seven databases were searched: Cochrane Library, PubMed, Web of Science, Embase, Wanfang, China Science Technology Journal, National Knowledge Infrastructure, spanning from inception April 15, 2024. Extraction Two authors independently searched using search terms. Analysis Following strict inclusion criteria, 8 papers ultimately included. suggests could enhance follicular development, promote proliferation differentiation granulosa cells, elevate estradiol levels, mitigate testosterone, C-reactive protein, ovarian index levels. Conclusion analysis dietary flavonoids potentially alleviate symptoms associated with Systematic Review Registration PROSPERO registration no. CRD42022382912.

Язык: Английский

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Serum PM20D1 levels is associated with nutritional status and inflammatory factors in gastric cancer patients undergoing early enteral nutrition

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 26, 2024

Background and Objective: Early nutritional support holds paramount importance for postoperative gastric cancer (GC) patients. Peptidase M20 domain containing 1 (PM20D1) is a secretory enzyme associated with glucose lipid metabolism. However, there dearth of clinical studies delving into the connection between PM20D1, metabolism, inflammatory factors in GC patients who have received enteral nutrition. This research aimed to investigate serum levels PM20D1 following early nutrition (EN) its potential associations markers, factors. Methods prospective observational study enrolled 180 May 2020 July 2022. On first day, all support, which was maintained duration 5 days. Serum interleukin (IL)-6, IL-1β, C-reactive protein (CRP) were measured on seventh day after surgery using enzyme-linked immunosorbent assay (ELISA). Data demographics, statistics, parameters, prognostic index (PNI) collected. Patients followed up 12 months, both overall survival (OS) disease-free (DFS) recorded. Results In low PNI group, albumin (ALB), blood lymphocytes (BL) showed significant reductions. Pearson analysis revealed negative correlation IL-6 levels, whereas positive emerged ALB BL levels. Furthermore, demonstrated as biomarker diagnosing poor status (PNI < 43) risk factor Conclusion remarkably declined EN status.

Язык: Английский

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The in vitro cytotoxicity, genotoxicity and LC-ToF-MS profiling of four South African plants with good antifungal activity

South African Journal of Botany, Год журнала: 2024, Номер 174, С. 446 - 455

Опубликована: Сен. 21, 2024

Язык: Английский

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