Transcriptome analysis of Stephania yunnanensis and functional validation of CYP80s involved in benzylisoquinoline alkaloids biosynthesis DOI Creative Commons

Wenlong Shi,

Qishuang Li, Xinyi Li

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Abstract The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloids (BIAs), with being the representative and most abundant compound, but our understanding on biosynthesis BIA this have been relatively limited. Previous research has reported genome S. preliminarily identified upstream gene norcoclaurine synthase (NCS) biosynthetic pathways. However, key genes promoting formation skeleton not yet reported. In study, based differences content crebanine several other BIAs different tissues, we conducted transcriptome sequencing roots, stems, leaves. We then candidate through functional annotation sequence alignment, followed by transcriptomic genomic analyses. Based analysis, three CYP80 enzymes (SyCYP80Q5-1, SyCYP80Q5-3, SyCYP80G6), which exhibited activities towards (S)- (R)-configured substrates yunnanensisand demonstrated strict stereoselectivity enroute to aporphine. This study provides metabolomic information offers valuable insights into elucidation biosynthesis, lays foundation for complete analysis pathways more alkaloids.

Язык: Английский

Ant colony algorithm-enabled back propagation neural network and response surface methodology based ultrasonic optimization of safflower seed alkaloid extraction and antioxidant DOI
Haofang Wan,

Saiya Zhou,

Chang Li

и другие.

Industrial Crops and Products, Год журнала: 2024, Номер 220, С. 119191 - 119191

Опубликована: Июль 16, 2024

Язык: Английский

Процитировано

32
Novel Multi-Antioxidant Approach for Ischemic Stroke Therapy Targeting the Role of Oxidative Stress DOI Creative Commons
Camilo Briones-Valdivieso,

Felipe Briones,

Sofía Orellana-Urzúa

и другие.

Biomedicines, Год журнала: 2024, Номер 12(3), С. 501 - 501

Опубликована: Фев. 23, 2024

Stroke is a major contributor to global mortality and disability. While reperfusion essential for preventing neuronal death in the penumbra, it also triggers cerebral ischemia-reperfusion injury, paradoxical injury primarily caused by oxidative stress, inflammation, blood–brain barrier disruption. An burst inflicts marked cellular damage, ranging from alterations mitochondrial function lipid peroxidation activation of intricate signalling pathways that can even lead cell death. Thus, given pivotal role stress mechanisms reinforcement antioxidant defence system has been proposed as protective approach. Although this strategy proven be successful experimental models, its translation into clinical practice yielded inconsistent results. However, should considered availability numerous molecules with wide range chemical properties affect extent injury; several groups molecules, including polyphenols, carotenoids, vitamins, among other compounds, mitigate damage intervening multiple at various stages. Multiple trials have previously conducted evaluate these using melatonin, acetyl-L-carnitine, chrysanthemum extract, edaravone dexborneol, saffron, coenzyme Q10, oleoylethanolamide, treatments. Therefore, multi-antioxidant therapy emerges promising novel therapeutic option due potential synergistic effect provided simultaneous roles individual compounds.

Язык: Английский

Процитировано

15
The Roles of RhoA/ROCK/NF-κB Pathway in Microglia Polarization Following Ischemic Stroke DOI

Weizhuo Lu,

Yilin Wang,

Jiyue Wen

и другие.

Journal of Neuroimmune Pharmacology, Год журнала: 2024, Номер 19(1)

Опубликована: Май 16, 2024

Язык: Английский

Процитировано

9
Polysaccharides extracted from common buckwheat (Fagopyrum esculentum) attenuate cognitive impairment via suppressing RAGE/p38/NF-κB signaling and dysbiosis in AlCl3-treated rats DOI
Yuchen Liu, Shengyi Chen, Yingying Chen

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 276, С. 133898 - 133898

Опубликована: Июль 15, 2024

Язык: Английский

Процитировано

4
Crebanine mitigates glucocorticoid‐induced osteonecrosis of the femoral head by restoring bone remodelling homeostasis via attenuating oxidative stress DOI Creative Commons

Shankun Dong,

Jianxun Ge,

Meng Qi

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(16)

Опубликована: Авг. 1, 2024

The onset of osteonecrosis the femoral head (ONFH) is intimately associated with extensive administration glucocorticoids (GCs). Long-term stimulation GCs can induce oxidative stress in both osteoclasts (OCs) and osteoblasts (OBs), resulting disturbance bone remodelling. An alkaloid named crebanine (CN) demonstrates pharmacological properties including anti-inflammation reactive oxygen species (ROS) modulation. Our objective to assess therapeutic potential CN treating ONFH elucidate underlying mechanisms. network pharmacology analysis uncovered that played a role regulating ROS metabolism. In vitro, demonstrated its ability reduce dexamethasone (DEX)-stimulated generation OCs suppress their resorptive function by downregulating level osteoclast marker genes. Concurrently, also mitigated DEX-induced damage OBs, facilitating restoration osteoblast gene expression, cellular differentiation function. These effects were achieved augmenting antioxidant system intracellular levels. Furthermore, vitro results corroborated micro-CT histological data, which showed attenuated MPS-induced mice. This study highlights counteracting GCs-induced ONFH.

Язык: Английский

Процитировано

4
Shedding light on microglial dysregulation in Alzheimer’s disease: exploring molecular mechanisms and therapeutic avenues DOI
Vinay Bhardwaj, Sneha Kumari,

Rishika Dhapola

и другие.

Inflammopharmacology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 28, 2024

Язык: Английский

Процитировано

4
ABBV-744 alleviates LPS-induced neuroinflammation via regulation of BATF2-IRF4-STAT1/3/5 axis DOI

Lele Wang,

Huan Wang,

Sijin Lin

и другие.

Acta Pharmacologica Sinica, Год журнала: 2024, Номер unknown

Опубликована: Июнь 11, 2024

Язык: Английский

Процитировано

3
Transcriptome Analysis of Stephania yunnanensis and Functional Validation of CYP80s Involved in Benzylisoquinoline Alkaloid Biosynthesis DOI Creative Commons

Wenlong Shi,

Qishuang Li, Xinyi Li

и другие.

Molecules, Год журнала: 2025, Номер 30(2), С. 259 - 259

Опубликована: Янв. 10, 2025

The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloid (BIA), with being the representative and most abundant compound, but our understanding biosynthesis BIAs this has been relatively limited. Previous research reported genome S. preliminarily identified norcoclaurine synthase (NCS), which involved early stages BIA biosynthetic pathways. However, key genes promoting formation skeleton have not yet reported. In study, based on differences content crebanine several other different tissues, we conducted transcriptome sequencing roots, stems, leaves. We then candidate through functional annotation sequence alignment further analyzed them combination genome. Based analysis, three CYP80 enzymes (SyCYP80Q5-1, SyCYP80Q5-3, SyCYP80G6), exhibited activities toward (S)- (R)-configured substrates demonstrated strict stereoselectivity enroute to aporphine. This study provides metabolomic transcriptomic information yunnanensis, offers valuable insights into elucidation biosynthesis, lays foundation for complete analysis pathways more alkaloids.

Язык: Английский

Процитировано

0
Features of NeuN Expression in the Cingulate Cortex of the Cerebral Hemispheres of Mice After Administration of Escherichia Coli Lipopolysaccharide DOI Open Access
А. А. Венедиктов

Journal of Anatomy and Histopathology, Год журнала: 2025, Номер 13(4), С. 22 - 28

Опубликована: Янв. 20, 2025

The aim was to observe changes in NeuN expression the cingulate cortex of cerebral hemispheres mice after intraperitoneal administration various doses bacterial lipopolysaccharide (LPS). Material and methods. study conducted on 12 C57Bl/6 mice, which were injected intraperitoneally at same time for 4 days with saline (control group) or E. coli LPS one following doses: 0.5 mg/kg/day (1st group), 1 (2nd 2 (3rd group). Brain sampling performed animals histological preparations a 5 µm thickness made stained antibodies NeuN. number NeuN-expressing cells hemispheres, Cg1 area/dorsal anterior cortex, counted. data also statistically processed. Results. In immunohistochemical study, neurons expressing 1st group (LPS dose mg/kg/day) 8226.9±336.94 per mm2, 2nd – 7889.4±211.83 3rd 7039.7±580.42 control 9985.6±576.75 mm2. Immunofluorescent revealed no difference between samples. Conclusion . When is administered 0.5–2 mg/kg/day, NeuN, marker mature neurons, decreases. obtained can be used create model age-related neurodegeneration.

Язык: Английский

Процитировано

0
Unlocking the biological potential of crebanine, oxocrebanine and dehydrocrebanine: Pharmacological activity and related molecular mechanisms DOI
Dinesh Kumar Patel

Fitoterapia, Год журнала: 2025, Номер 182, С. 106467 - 106467

Опубликована: Март 7, 2025

Язык: Английский

Процитировано

0