New endophytic fungal species of Chaetomiaceae (Ascomycota) in Iraq

Biodiversitas Journal of Biological Diversity, Год журнала: 2023, Номер 24(10)

Опубликована: Ноя. 2, 2023

Abstract. Al-Rifaie AA, Ameen MKM. 2023. New endophytic fungal species of Chaetomiaceae (Ascomycota) in Iraq. Biodiversitas 24: 5270-5277. The present study was conducted to isolate and identify some fungi from vegetable crops Samples eight plants, namely Abelmoschus esculentus, Mentha piperita, Vicia faba, Petroselinum sativum, Ocimum basilicum, Lawsonia inermis, Beta vulgaris Apium graveolens were collected three regions Basrah (Abu Al-Khaseeb, Karmat Ali the Centre Basrah). Isolation done by solid culture method moist method. recovered purified axenic cultures each isolated then identified on basis their macro micro-morphological features. Morphological identification further confirmed molecular analysis through DNA extraction sequencing PCR amplification ITS4 ITS5 gene primers. Phylogenetic examination revealed that five novel related family including Chaetomium cucumericola, C. madrasense, Amesia atrobrunnea, A. cymbiformis Botryotichum verrucosum. Three species, B. verrucosum, documented for first time Iraqi mycobiota. To best our acknowledgment, this is investigating vegetables

Язык: Английский

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Novel chlorinated and nitrogenated azaphilones with cytotoxic activities from the marine algal-derived fungus Chaetomium globosum 2020HZ23

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Авг. 21, 2023

Two novel chlorinated and nitrogenated azaphilones, namely N -butyl-2-aza-2-deoxychaetoviridin A ( 1 ) -hexyl-2-aza-2-deoxychaetoviridin 2 ), along with a previously identified analogue, chaetoviridin 3 were successfully obtained from Chaetomium globosum 2020HZ23, marine algal-sourced endophytic fungus. The planar structures as well the absolute configurations of these new metabolites determined utilizing synergistic approach that involved both spectroscopic techniques (1D/2D NMR HRESIMS) Density Functional Theory (DFT) calculations. Each compound was subject to in vitro cytotoxicity evaluation toward A549 cancer cell line. Both compounds demonstrated significant cytotoxicity, evidenced by their respective IC 50 values 13.6 17.5 μM. Furthermore, potent migration inhibition, which elevated increasing dose concentration. In contrast, exhibited less cytotoxic activity relative , suggesting potency escalates N-substitution at C-2 position introduction side chain. This finding could offer implications for future studies aimed designing refining lead within this class.

Язык: Английский

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Endophyte Chaetomium, Potential Bioactivity: Pharmaceutical and Phytochemical Analyses

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 2, 2023

Abstract Endophytic fungi establish mutualistic symbiotic relationships with plants, wherein their interactions facilitate the production of bioactive secondary metabolites by fungal cells. The objective this study is to examine antioxidative, anticancer, cytotoxic, and antiviral properties crude from endophytes Chaetomium laterale LC4146 interruptum CBS126660. Chromatographic separation metabolic main chemical identification were also addressed. Semi-purified extracts showed radical-inhibiting or 2,2-diphenyl-1-picrylhydrazyl-radical-scavenging capabilities. ethyl acetate extract C . has highest inhibitory activity against human liver (HepG2), prostate (PC-3), colorectal (HCT-116) cancer cells interestingly shows low toxicity normal Vero African Green Monkey kidney Fungi exerted dose-dependent anticancer effects on three cell lines, observable alterations in shape morphology HepG2 under an inverted microscope. To test vitro anti- Herpes Simplex Virus type-1 (HSV-1) efficiency petroleum ether extracts, cytopathic effect inhibition was weak moderate. Phytochemical examination revealed phenolics, esters, alcohols, hydrocarbons. Hexanedioic acid, 2,4-ditert-butylphenol, 1,2-benzene dicarboxylic 1-hexadecanol are most successful chemicals. Since these unique components acceptable , two species may be a viable source natural products good starting point for medicinal applications.

Язык: Английский

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Antifungal metabolites of biocontrol stain LB-1 and their inhibition mechanism against Botrytis cinerea

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Сен. 4, 2024

LB-1 is a novel biocontrol strain that produces non-volatile metabolites inhibit the growth of

Язык: Английский

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Soil Fusarium graminearum is inhibited by combined application of crop residues and an organic stimulant

Applied Soil Ecology, Год журнала: 2024, Номер 203, С. 105643 - 105643

Опубликована: Сен. 16, 2024

Язык: Английский

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