Israel Journal of Chemistry,
Год журнала:
2024,
Номер
64(5)
Опубликована: Май 1, 2024
Abstract
N
6
‐methyladenosine
(m
A),
as
the
most
abundant
and
well‐studied
RNA
modification,
can
be
reversibly
added
or
removed
by
m
A
methyltransferase
demethylase.
The
further
molecular
biological
function
of
is
achieved
recognition
its
binding
protein.
functions
in
diverse
progress
processing,
including
transcription
regulation,
splicing,
nuclear
export,
stability,
translation,
to
regulate
fate
cells.
Although
been
extensively
studied
various
animal
cell
systems,
research
on
A's
regulatory
plant
cells
lags.
In
recent
years,
with
a
deepening
understanding
development
sequencing
technologies,
researches
have
gradually
increased.
this
review,
we
focused
discussing
nucleus
cytoplasm,
aiming
elucidate
specific
mechanisms
which
regulates
RNAs
plants.
Finally,
provide
some
perspectives
future
investigations
detailed
mechanism
A‐mediated
regulation
plants,
might
insights
into
strategies
for
achieving
multiple
growth
processes
crops.
Plant Communications,
Год журнала:
2024,
Номер
5(10), С. 101037 - 101037
Опубликована: Июль 6, 2024
N6-methyladenosine
(m6A)
is
the
most
abundant
modification
observed
in
eukaryotic
mRNAs.
Advances
transcriptome-wide
m6A
mapping
and
sequencing
technologies
have
enabled
identification
of
several
conserved
motifs
plants,
including
RRACH
(R
=
A/G
H
A/C/U)
UGUAW
(W
U
or
A)
motifs.
However,
mechanisms
underlying
deposition
marks
at
specific
positions
individual
transcripts
remain
to
be
clarified.
Evidence
from
plant
animal
studies
suggests
that
writer
eraser
components
are
recruited
genomic
loci
through
interactions
with
particular
transcription
factors,
5-methylcytosine
DNA
methylation
marks,
histone
marks.
In
addition,
recent
cells
shown
microRNAs
play
a
role
depositing
sites
base-pairing
mechanism.
also
affects
biogenesis
function
chromatin-associated
regulatory
RNAs
long
noncoding
RNAs.
Although
we
less
an
understanding
link
between
epigenetic
factors
plants
than
animals,
progress
identifying
proteins
interact
has
provided
insights
into
crosstalk
which
plays
crucial
transcript-specific
regulation
plants.
Plant Cell & Environment,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 2, 2025
Salt
stress
can
seriously
affect
plant
survival.
To
adapt
to
salt
stress,
plants
alter
gene
expressions
and/or
pre-mRNA
processing
patterns,
or
both.
Previous
studies
could
not
comprehensively
profile
stress-responsive
patterns
due
limitations
in
traditional
sequencing
technologies.
Now
Oxford
Nanopore
Technologies
Direct
RNA
Sequencing
(ONT
DRS)
directly
sequence
full-length
native
RNAs
without
requiring
reverse
transcription
amplification.
Thus,
it
provides
accurate
profiles
of
at
the
single-molecule
level.
With
this
technology,
we
found
more
than
89
586
novel
transcript
isoforms
addition
44
877
annotated
ones
soybean
leaves
and
roots
subjected
short-term
stress.
Specifically,
identified
102
191
alternative
mRNA
events
1216
fusion
transcripts
corresponding
549
genomic
regions.
Interestingly,
genes
upregulated
had
longer
poly(A)
tail
lengths
lower
m6A
modification
ratios
controls,
downregulated
shorter
tails.
Also,
levels
changed
with
prolonged
Furthermore,
alteration
modifications
under
were
correlated
two
erasers.
Our
results
indicated
that
reshaped
traits
caused
by
play
a
role
adaptations.
Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
66(12), С. 2586 - 2599
Опубликована: Окт. 4, 2024
ABSTRACT
Modifications
to
RNA
have
recently
been
recognized
as
a
pivotal
regulator
of
gene
expression
in
living
organisms.
More
than
170
chemical
modifications
identified
RNAs,
with
N
6
‐methyladenosine
(m
A)
being
the
most
abundant
modification
eukaryotic
mRNAs.
The
addition
and
removal
m
A
marks
are
catalyzed
by
methyltransferases
(referred
“writers”)
demethylases
“erasers”),
respectively.
In
addition,
mRNAs
interpreted
A‐binding
proteins
“readers”),
which
regulate
fate
mRNAs,
including
stability,
splicing,
transport,
translation.
Therefore,
exploring
mechanism
underlying
reader‐mediated
modulation
metabolism
is
essential
for
much
deeper
understanding
epigenetic
role
plants.
Recent
discoveries
improved
our
functions
readers
plant
growth
development,
stress
response,
disease
resistance.
This
review
highlights
latest
developments
reader
research,
emphasizing
diverse
RNA‐binding
domains
crucial
function
biological
cellular
roles
response
developmental
environmental
signals.
Moreover,
we
propose
discuss
potential
future
research
directions
challenges
identifying
novel
elucidating
mechanistic
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Июль 16, 2024
The
epitranscriptomic
mark
N
6-methyladenosine
(m6A)
is
the
most
common
type
of
messenger
RNA
(mRNA)
post-transcriptional
modification
in
eukaryotes.
With
discovery
demethylase
FTO
(FAT
MASS
AND
OBESITY-ASSOCIATED
PROTEIN)
Homo
Sapiens,
this
has
been
proven
to
be
dynamically
reversible.
technological
advances,
research
on
m6A
plants
also
rapidly
developed.
widely
distributed
plants,
which
usually
enriched
near
stop
codons
and
3'-UTRs,
conserved
sequences.
related
proteins
mainly
consist
three
components:
methyltransferases
(writers),
demethylases
(erasers),
reading
(readers).
regulates
growth
development
by
modulating
metabolic
processes
playing
an
important
role
their
responses
environmental
signals.
In
review,
we
briefly
outline
detection
techniques;
comparatively
analyze
distribution
characteristics
plants;
summarize
methyltransferases,
demethylases,
binding
m6A;
elaborate
how
functions
plant
growth,
development,
response
signals;
provide
a
summary
outlook
plants.
Physiologia Plantarum,
Год журнала:
2025,
Номер
177(1)
Опубликована: Янв. 1, 2025
Abstract
N6‐methyladenosine
(m
6
A),
the
most
prevalent
modification
found
in
eukaryotic
mRNAs,
is
recognized
and
interpreted
by
m
A‐binding
proteins
called
A
readers.
The
EVOLUTIONARILY
CONSERVED
C‐TERMINAL
REGION
(ECT)
have
increasingly
been
identified
as
readers
plants.
recent
study
has
demonstrated
that
loss‐of‐function
ect8
mutant
sensitive
to
salt
stress
enhancing
stability
of
negative
regulators
Arabidopsis
(
thaliana
).
In
this
study,
we
generated
analyzed
ECT8‐overexpressing
transgenic
plants
further
explore
function
ECT8
response.
electrophoretic
mobility
shift
assay
vitro
showed
binds
A‐modified
synthetic
RNAs,
preferring
UGUm
AA
UACm
AGA
motifs
over
GGm
ACU
motif.
Contrary
exhibiting
hypersensitivity
regulators,
displayed
tolerance
increasing
expression
levels
positive
regulators.
Moreover,
RNA‐immunoprecipitation
stress‐responsive
mRNAs
planta
.
Collectively,
our
current
previous
findings
highlight
ECT8‐mediated
stabilization
destabilization
genes
encoding
or
respectively,
contribute
Arabidopsis.
