Cancers,
Год журнала:
2023,
Номер
15(10), С. 2676 - 2676
Опубликована: Май 10, 2023
The
complex
molecular
alterations
that
underlie
cancer
pathophysiology
are
studied
in
depth
with
omics
methods
using
bulk
tissue
extracts.
For
spatially
resolved
diagnostics
needle
biopsy
cores,
however,
histopathological
analysis
stained
FFPE
and
the
immunohistochemistry
(IHC)
of
a
few
marker
proteins
is
currently
main
clinical
focus.
Today,
spatial
imaging
MSI
or
IRI
an
emerging
diagnostic
technology
for
identification
classification
various
types.
However,
to
conserve
tissue-specific
metabolomic
states,
fast,
reliable,
precise
preparation
fresh-frozen
(FF)
sections
crucial.
Such
often
incompatible
practice,
since
metabolomics
routine
histopathology
biopsies
require
two
FF
sampling,
respectively.
Therefore,
we
developed
device
corresponding
laboratory
computational
workflows
multimodal
fresh-frozen,
longitudinally
sectioned
accompany
standard
same
core.
As
proof-of-concept,
analyzed
surgical
human
liver
specimens
co-registration
and,
following
processing,
by
sequential
pathology
This
workflow
allowed
comparison
between
different
spectral
profiles
histology,
as
well
direct
histological
diagnosis
without
need
extra
biopsy.
Clinical and Translational Medicine,
Год журнала:
2025,
Номер
15(4)
Опубликована: Март 28, 2025
Abstract
Atherosclerosis
is
a
chronic
inflammatory
condition
of
the
arteries,
marked
by
development
plaques
within
arterial
intima.
The
rupture
unstable
can
lead
to
thrombosis,
downstream
vessel
occlusion
and
serious
clinical
events.
composition
atherosclerotic
complex
highly
heterogeneous,
posing
challenges
for
their
study.
current
pathology
histological
subtype
classification
may
fail
fully
encompass
microscopic
molecular
components
in
tissue,
disease
progress
various
stages
atherosclerosis
potential
mechanism
plaque
rupture.
However,
spatial
mapping
heterogeneity
tissue
enhance
our
understanding
these
lesions.
Despite
considerable
made
traditional
omics
field
research,
its
status
as
an
indispensable
technology,
there
remain
inherent
limitations
investigation
minute
molecular.
In
recent
years,
techniques
have
advanced
significantly,
enabling
visualisation
analysis
specific
that
serve
causal
targets
associated
with
progression.
effective
application
both
research
settings
represents
promising
area
further
exploration.
This
review
focuses
on
advancements
findings
related
study
extracranial
carotid
cerebrovascular
disease.
Spatial
facilitate
detection
biomarkers
diagnostic
significance
or
relevance
disease,
offering
new
methods
insights
into
diagnosis
complications.
Mass Spectrometry Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 29, 2025
ABSTRACT
Lipopolysaccharides
(LPS)
are
complex
molecules
embedded
in
the
outer
membrane
of
Gram‐negative
bacteria.
LPS
highly
heterogeneous
across
species
and
strains,
posing
a
significant
analytical
challenge.
This
review
article
explores
recent
advances
identification
characterization
LPS,
with
particular
focus
on
role
mass
spectrometry
(MS)
techniques.
The
highlights
how
MS,
conjunction
various
separation
methods
spanning
different
ionization
techniques
dissociation
modes,
has
enabled
more
precise
sensitive
determination
composition,
lipid
A
structure.
Finally,
emerging
trends
MS
applications
for
research
discussed
its
potential
to
provide
deeper
insights
into
development
antibiotic
resistance
among
pathogenic
Analytical Chemistry,
Год журнала:
2023,
Номер
95(47), С. 17337 - 17346
Опубликована: Окт. 27, 2023
Technologies
assessing
the
lipidomics,
genomics,
epigenomics,
transcriptomics,
and
proteomics
of
tissue
samples
at
single-cell
resolution
have
deepened
our
understanding
physiology
pathophysiology
an
unprecedented
level
detail.
However,
study
spatial
metabolomics
in
undecalcified
bones
faces
several
significant
challenges,
such
as
fragility
bone,
which
often
requires
decalcification
or
fixation
leading
to
degradation
removal
lipids
other
molecules.
As
such,
we
describe
a
method
for
performing
mass
spectrometry
imaging
on
spine
that
is
compatible
with
omics
measurements.
In
brief,
use
fresh-frozen
rat
spines
system
carboxyl
methylcellulose
embedding,
cryofilm,
polytetrafluoroethylene
rollers
maintain
integrity
while
avoiding
signal
loss
from
variations
laser
focus
artifacts
traditional
processing.
This
reveals
various
types
lipidomic
profiles
spinal
regions
10
μm
resolutions
using
matrix-assisted
desorption/ionization
imaging.
We
expect
this
be
adapted
applied
analysis
cord,
shedding
light
mechanistic
aspects
cellular
heterogeneity,
development,
disease
pathogenesis
underlying
different
bone-related
conditions
diseases.
furthers
methodology
high
adds
collective
efforts
achieve
holistic
diseases
via
multiomics.
Cancers,
Год журнала:
2023,
Номер
15(10), С. 2676 - 2676
Опубликована: Май 10, 2023
The
complex
molecular
alterations
that
underlie
cancer
pathophysiology
are
studied
in
depth
with
omics
methods
using
bulk
tissue
extracts.
For
spatially
resolved
diagnostics
needle
biopsy
cores,
however,
histopathological
analysis
stained
FFPE
and
the
immunohistochemistry
(IHC)
of
a
few
marker
proteins
is
currently
main
clinical
focus.
Today,
spatial
imaging
MSI
or
IRI
an
emerging
diagnostic
technology
for
identification
classification
various
types.
However,
to
conserve
tissue-specific
metabolomic
states,
fast,
reliable,
precise
preparation
fresh-frozen
(FF)
sections
crucial.
Such
often
incompatible
practice,
since
metabolomics
routine
histopathology
biopsies
require
two
FF
sampling,
respectively.
Therefore,
we
developed
device
corresponding
laboratory
computational
workflows
multimodal
fresh-frozen,
longitudinally
sectioned
accompany
standard
same
core.
As
proof-of-concept,
analyzed
surgical
human
liver
specimens
co-registration
and,
following
processing,
by
sequential
pathology
This
workflow
allowed
comparison
between
different
spectral
profiles
histology,
as
well
direct
histological
diagnosis
without
need
extra
biopsy.
