Mass Spectrometry Reviews,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 24, 2024
Abstract
The
use
of
matrix‐assisted
laser
desorption/ionization
(MALDI)
mass
spectrometry
for
the
analysis
carbohydrates
and
glycoconjugates
is
a
well‐established
technique
this
review
12
th
update
original
article
published
in
1999
brings
coverage
literature
to
end
2022.
As
with
previous
review,
also
includes
few
papers
that
describe
methods
appropriate
by
MALDI,
such
as
sample
preparation,
even
though
ionization
method
not
MALDI.
follows
same
format
reviews.
It
divided
into
three
sections:
(1)
general
aspects
theory
MALDI
process,
matrices,
derivatization,
imaging,
fragmentation,
quantification
computer
software
structural
identification.
(2)
Applications
various
types
oligo‐
polysaccharides,
glycoproteins,
glycolipids,
glycosides
biopharmaceuticals,
(3)
other
areas
medicine,
industrial
processes,
natural
products
glycan
synthesis
where
extensively
used.
Much
material
relating
applications
presented
tabular
form.
still
an
ideal
carbohydrate
analysis,
particularly
its
ability
produce
single
ions
from
each
analyte
advancements
range
show
little
sign
diminishing.
Mass Spectrometry Reviews,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 29, 2025
ABSTRACT
Lipopolysaccharides
(LPS)
are
complex
molecules
embedded
in
the
outer
membrane
of
Gram‐negative
bacteria.
LPS
highly
heterogeneous
across
species
and
strains,
posing
a
significant
analytical
challenge.
This
review
article
explores
recent
advances
identification
characterization
LPS,
with
particular
focus
on
role
mass
spectrometry
(MS)
techniques.
The
highlights
how
MS,
conjunction
various
separation
methods
spanning
different
ionization
techniques
dissociation
modes,
has
enabled
more
precise
sensitive
determination
composition,
lipid
A
structure.
Finally,
emerging
trends
MS
applications
for
research
discussed
its
potential
to
provide
deeper
insights
into
development
antibiotic
resistance
among
pathogenic
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 31, 2024
Abstract
Spatial
metabolomics
using
imaging
mass
spectrometry
(MS)
enables
untargeted
and
label-free
metabolite
mapping
in
biological
samples.
Despite
the
range
of
available
MS
protocols
technologies,
our
understanding
detection
under
specific
conditions
is
limited
due
to
sparse
empirical
data
predictive
theories.
Consequently,
challenges
persist
designing
new
experiments,
accurately
annotating
interpreting
data.
In
this
study,
we
systematically
measured
detectability
172
biologically-relevant
metabolites
across
common
custom
reference
We
evaluated
24
MALDI-imaging
for
metabolomics,
demonstrated
applicability
findings
complex
samples
through
comparison
with
animal
tissue
showcased
potential
extending
results
further
analytes
by
predicting
based
on
molecular
properties.
Additionally,
interlaboratory
10
including
MALDI,
DESI,
IR-MALDESI,
showed
extensive
coverage
comparable
results,
underscoring
broad
within
community.
share
a
interactive
web
application
integrated
METASPACE.
This
resource
offers
an
catalogue
detectable
ions,
facilitating
protocol
selection,
supporting
annotation,
benefiting
future
spatial
studies.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(47), P. 17337 - 17346
Published: Oct. 27, 2023
Technologies
assessing
the
lipidomics,
genomics,
epigenomics,
transcriptomics,
and
proteomics
of
tissue
samples
at
single-cell
resolution
have
deepened
our
understanding
physiology
pathophysiology
an
unprecedented
level
detail.
However,
study
spatial
metabolomics
in
undecalcified
bones
faces
several
significant
challenges,
such
as
fragility
bone,
which
often
requires
decalcification
or
fixation
leading
to
degradation
removal
lipids
other
molecules.
As
such,
we
describe
a
method
for
performing
mass
spectrometry
imaging
on
spine
that
is
compatible
with
omics
measurements.
In
brief,
use
fresh-frozen
rat
spines
system
carboxyl
methylcellulose
embedding,
cryofilm,
polytetrafluoroethylene
rollers
maintain
integrity
while
avoiding
signal
loss
from
variations
laser
focus
artifacts
traditional
processing.
This
reveals
various
types
lipidomic
profiles
spinal
regions
10
μm
resolutions
using
matrix-assisted
desorption/ionization
imaging.
We
expect
this
be
adapted
applied
analysis
cord,
shedding
light
mechanistic
aspects
cellular
heterogeneity,
development,
disease
pathogenesis
underlying
different
bone-related
conditions
diseases.
furthers
methodology
high
adds
collective
efforts
achieve
holistic
diseases
via
multiomics.
Metabolites,
Journal Year:
2024,
Volume and Issue:
14(3), P. 173 - 173
Published: March 20, 2024
Urinary
tract
cancers,
including
those
of
the
bladder,
kidneys,
and
prostate,
represent
over
12%
all
with
significant
global
incidence
mortality
rates.
The
continuous
challenge
that
these
cancers
present
necessitates
development
innovative
diagnostic
prognostic
methods,
such
as
identifying
specific
biomarkers
indicative
cancer.
Biomarkers,
which
can
be
genes,
proteins,
metabolites,
or
lipids,
are
vital
for
various
clinical
purposes
early
detection
prognosis.
Mass
spectrometry
(MS),
particularly
soft
ionization
techniques
electrospray
(ESI)
laser
desorption/ionization
(LDI),
has
emerged
a
key
tool
in
metabolic
profiling
biomarker
discovery,
due
to
its
high
resolution,
sensitivity,
ability
analyze
complex
biological
samples.
Among
LDI
techniques,
matrix-assisted
(MALDI)
surface-assisted
(SALDI)
should
mentioned.
While
MALDI
methodology,
uses
organic
compounds
matrices,
is
effective
larger
molecules,
SALDI,
based
on
types
nanoparticles
nanostructures,
preferred
smaller
metabolites
lipids
reduced
spectral
interference.
This
study
highlights
application
along
mass
imaging
(MSI),
potential
lipid
urological
focusing
most
common
kidney,
prostate
cancers.
Mass Spectrometry Reviews,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 24, 2024
Abstract
The
use
of
matrix‐assisted
laser
desorption/ionization
(MALDI)
mass
spectrometry
for
the
analysis
carbohydrates
and
glycoconjugates
is
a
well‐established
technique
this
review
12
th
update
original
article
published
in
1999
brings
coverage
literature
to
end
2022.
As
with
previous
review,
also
includes
few
papers
that
describe
methods
appropriate
by
MALDI,
such
as
sample
preparation,
even
though
ionization
method
not
MALDI.
follows
same
format
reviews.
It
divided
into
three
sections:
(1)
general
aspects
theory
MALDI
process,
matrices,
derivatization,
imaging,
fragmentation,
quantification
computer
software
structural
identification.
(2)
Applications
various
types
oligo‐
polysaccharides,
glycoproteins,
glycolipids,
glycosides
biopharmaceuticals,
(3)
other
areas
medicine,
industrial
processes,
natural
products
glycan
synthesis
where
extensively
used.
Much
material
relating
applications
presented
tabular
form.
still
an
ideal
carbohydrate
analysis,
particularly
its
ability
produce
single
ions
from
each
analyte
advancements
range
show
little
sign
diminishing.
