AFK-PD alleviated osteoarthritis progression by chondroprotective and anti-inflammatory activity DOI Creative Commons
Zhuang Qian, Jie Xu, Lei Zhang

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Авг. 29, 2024

Osteoarthritis (OA) is the most prevalent cartilage degenerative and low-grade inflammatory disease of whole joint. However, there are currently no FDA-approved drugs or global regulatory agency-approved treatments OA modification. Therefore, it’s essential to explore novel effective therapeutic strategies for OA. In our study, we investigated effects AFK-PD, a pyridone agent, on development induced by destabilization medial meniscus (DMM) in vivo , its impact function chondrocytes treated with IL-1β vitro . Our results demonstrated AFK-PD alleviated progression through inhibiting degeneration, articular inflammation osteophyte formation. Notably, inhibited chondrocyte synovial macrophage M1 polarization, leading attenuation inflammation. Additionally, promoted anabolism while mitigating catabolism apoptosis, effectively degeneration. Mechanistically, suppressed expression key signaling molecules involved MAPK pathway, such as p-ERK1/2 p-JNK, well NF-κB molecule p-p65, IL-1β-induced chondrocytes. These findings suggest ameliorates protecting functions macrophages. Overall, study highlights promising candidate treatment

Язык: Английский

Characterization of rectus femoris lesions in knee osteoarthritis at different stages and the effect of ultrasound-guided acupotomy DOI Creative Commons
Wenying Yu, Jing Liu,

Ze-Hao Lin

и другие.

Frontiers in Physiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 23, 2025

Introduction Chronic injury to the rectus femoris muscle induces and exacerbates progression of knee osteoarthritis (KOA). However, lesion characteristics in KOA at different stages have not been fully characterized. The aim this study was analyze pattern investigate mechanism by which ultrasound-guided acupotomy operations can prevent control KOA. Methods Early, middle, late-stage rabbit models were constructed using modified Videman method. Ultrasonography used record elastic modulus cross-sectional area muscle, morphology observe ultramicroscopic changes assess degree fibrosis. Additionally, performed on model KOA, alterations key molecular markers fibrosis determined Western Blot qPCR methods. Results As disease progressed, rabbits gradually increased, decreased, increased. In contrast, improved after intervention. Conclusion These findings highlight gradual increase elasticity, decrease area, increased as progressed. Ultrasoundguided shown a protective effect cartilage delay ameliorating pathological muscle. may involve reducing chronic protecting joint homeostasis attenuating

Язык: Английский

Процитировано

1
LIPUS Promotes Calcium Oscillation and Enhances Calcium Dependent Autophagy of Chondrocytes to Alleviate Osteoarthritis DOI Creative Commons
Mengtong Guan, Xiaoyu Han, Bo Liao

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Abstract Osteoarthritis (OA) is a degenerative disease which places an enormous burden on society, effective treatments are still limited. As non‐invasive and safe physical therapy, low‐intensity pulsed ultrasound (LIPUS) can alleviate OA progression, but the underlying mechanism not fully understood, especially mechanical transduction between LIPUS organism. In this pioneering study, biomechanical effects of living mice chondrocytes body zebrafish investigate by using fluorescence imaging technology, to dynamically “visualize” its invisible stimuli in form calcium oscillations. It also confirmed that maintains cartilage homeostasis promoting chondrocyte autophagy calcium‐dependent manner. addition, ion channels screened scRNA‐seq confirm mechanosensitive channel transient receptor potential vanilloid 4 (TRPV4) mediated biological chondrocytes. Finally, it found combination pharmacologically induced LIPUS‐induced Ca 2+ influx enhances cartilage‐protective effect LIPUS, may provide new insights for optimizing treatment OA.

Язык: Английский

Процитировано

0
Aberrant anabolism hinders constructive metabolism of chondrocytes by pharmacotherapy in osteoarthritis DOI Creative Commons
Raquel Largo, Aránzazu Mediero, Cristina Villa-Gómez

и другие.

Bone and Joint Research, Год журнала: 2025, Номер 14(3), С. 199 - 207

Опубликована: Март 5, 2025

Osteoarthritis (OA) is a highly prevalent and disabling disease with an unmet therapeutic need. The characteristic cartilage loss alteration of other joint structures result from complex interaction multiple risk factors, mechanical overload consistently playing central role. This generates inflammatory response in the due to activation innate immune chondrocytes, which occurs through various cellular mechanisms. Moreover, factors associated obesity, being overweight, metabolic syndrome enhance both locally systemically. OA only cells present articular cartilage, are therefore inflamed initiate anabolic process attempt repair damaged tissue, ultimately results aberrant dysfunctional process. Under these circumstances, where continues be subjected chronic stress, proposing treatment that stimulates chondrocytes' restore tissue structure does not appear target high likelihood success. In fact, drugs proposed for have yet demonstrate efficacy. By contrast, strategies focused on pharmacologically managing component, at systemic levels, shown promise. Therefore, prioritizing control pro-inflammatory pathways presents most viable promising strategy effective management OA. As research continues, this approach may offer best opportunity alleviate burden incapacitating disease.

Язык: Английский

Процитировано

0
Cathepsin K Inhibitors as Potential Drugs for the Treatment of Osteoarthritis DOI Open Access

Leyre Brizuela,

René Buchet, Carole Bougault

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2896 - 2896

Опубликована: Март 22, 2025

Links between cathepsin K and the pathophysiology of osteoarthritis (OA) can be established, not least because overabundance in serum OA patients upregulation degraded cartilage animal models OA. Chondrocytes, chondroclasts, or osteoclasts contribute to accumulated at diseased osteochondral junction. After a general presentation physiology, as well its degradation processes, we describe function effect on via type II collagen cleavage. An overview most promising inhibitors is then presented, together with their vitro effects. Although intensive research initially focused bone resorption, there growing interest potential these drugs prevent degradation. In this review, summarize pre-clinical clinical trials that support use treatment To date, no molecules are commercially available, although few have undergone trials, but believe development could broaden therapeutic arsenal for

Язык: Английский

Процитировано

0
SHP2-mediated ROS activation induces chondrocyte paraptosis in osteoarthritis and is attenuated by low-intensity pulsed ultrasound DOI Creative Commons
Wenjie Hou,

Xingru Shang,

Xiaoxia Hao

и другие.

Journal of Orthopaedic Translation, Год журнала: 2025, Номер 52, С. 233 - 248

Опубликована: Апрель 25, 2025

Язык: Английский

Процитировано

0
TNF induces catabolism in human cartilaginous endplate cells in 3D agarose culture under dynamic compression DOI Creative Commons
Katherine B. Crump, Exarchos Kanelis, Maria Segarra-Queralt

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Май 6, 2025

Abstract Intervertebral disc (IVD) degeneration is the leading cause of low back pain in young adults, and cartilaginous endplate (CEP) likely to play a key role early IVD degeneration. To elucidate effects pro-inflammatory cytokines on mechanobiology CEP, human CEP cells were seeded into 2% agarose, dynamically compressed up 7%, stimulated with tumor necrosis factor (TNF). It was hypothesized that dynamic compression would be sufficient induce anabolism, while stimulation TNF catabolism. catabolic, time-dependent response through downregulation anabolic gene expression increased secretion proteins associated herniated discs, bacteria inhibition, pain. However, 7% strain or scaffold material, may not lead full activation integrins pathways, demonstrated part unchanged integrin subunits α 5 β 1 .

Язык: Английский

Процитировано

0
A wearable knee rehabilitation system based on graphene textile composite sensor: Implementation and validation DOI
Cheng Shen, Zhongcai Pei, Weihai Chen

и другие.

Engineering Applications of Artificial Intelligence, Год журнала: 2024, Номер 136, С. 108954 - 108954

Опубликована: Июль 13, 2024

Язык: Английский

Процитировано

2
Association between sarcopenic obesity and osteoarthritis: The potential mediating role of insulin resistance DOI Creative Commons
Zijian Li,

S. Yin,

Gang Zhao

и другие.

Experimental Gerontology, Год журнала: 2024, Номер 197, С. 112611 - 112611

Опубликована: Окт. 21, 2024

Sarcopenic obesity (SO) and osteoarthritis (OA) are highly prevalent musculoskeletal conditions that significantly impair health-related quality of life.

Язык: Английский

Процитировано

2
Transcriptome combined with single cell to explore hypoxia-related biomarkers in osteoarthritis DOI
Xingyu Liu, Guangdi Li,

Riguang Liu

и другие.

Journal of Chromatography B, Год журнала: 2024, Номер 1246, С. 124274 - 124274

Опубликована: Авг. 15, 2024

Язык: Английский

Процитировано

1
Inhibitory Effects of Reynoutria japonica Houtt. on Pain and Cartilage Breakdown in Osteoarthritis Based on Its Multifaceted Anti-Inflammatory Activity: An In Vivo and In Vitro Approach DOI Open Access
Hee-Geun Jo,

Chae Yun Baek,

Juni Lee

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10647 - 10647

Опубликована: Окт. 3, 2024

In the past 30 years, number of years lived with disability due to osteoarthritis (OA) has doubled, making it an increasing global health burden. To address this issue, interventions that inhibit progressive pathology driven by age-related low-grade inflammation, primary mechanism OA, are being actively pursued. Recent investigations have focused on modulating inflammatory disease as a therapeutic target. However, no agent successfully halted disease’s progression or reversed its irreversible course. Reynoutria japonica Houtt. (RJ), promising East Asian herbal medicine, been utilized for several diseases potent anti-inflammatory activity. This study aims determine RJ’s capacity OA symptoms and associated exploring potential further development. vivo in vitro experiments demonstrated anti-OA activity modulation multifaceted targets. RJ significantly inhibited pain, gait deterioration, cartilage destruction monosodium iodoacetate-induced rat model, analgesic effect confirmed acetic acid-induced writhing model. exhibited consistent against multiple targets serum model lipopolysaccharide-induced RAW 264.7 cells. The inhibition cytokines, including interleukin-1β, interleukin-6, matrix metalloproteinase-13, tumor necrosis factor-α, nitric oxide synthase 2, suggests alleviation manifestations relates These results indicate merits investigation disease-modifying drug candidate targeting OA’s pathology. characterize pharmacological properties RJ, future studies expanded designs warranted.

Язык: Английский

Процитировано

1