A GDP-mannose-1-phosphate guanylyltransferase as a potential HIGS target against Sclerotinia sclerotiorum

PLoS Pathogens, Год журнала: 2025, Номер 21(5), С. e1013129 - e1013129

Опубликована: Май 2, 2025

Sclerotinia stem rot is a devastating disease affecting vegetables and oil crops worldwide. It caused by the necrotrophic ascomycete ( S. ) sclerotiorum. Host-induced gene silencing (HIGS) has shown promise in control against insects fungal pathogens, but effective HIGS target genes sclerotiorum remain limited. In this study, we identified GDP-mannose pyrophosphorylase (GMPP) SsMPG2 through forward genetic analysis. Ssmpg2 mutants exhibit abnormal sclerotia compound appressoria, along with defective cell wall integrity attenuated virulence. Meanwhile, knocking out reduced GMPP activity glycosylation of proteins. addition, interacts SsMPG1, which essential . Downstream SsMPG1-SsMPG2 complex, SsPMT4 , encodes an O-mannosyltransferase, also critical for appressoria formation Notably, MPG2 virulence several other pathogens such as Botrytis cinerea Magnaporthe oryzae Fusarium graminearum Furthermore, expressing hairpin RNAs SsMPG1 Nicotiana benthamiana Arabidopsis thaliana significantly symptoms Collectively, our findings demonstrate roles phytopathogenic fungi suggest that MPGs are promising HlGS targets controlling

Язык: Английский

Ornithine enhances common bean growth and defense against white mold disease via interfering with SsOAH and diminishing the biosynthesis of oxalic acid in Sclerotinia sclerotiorum

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Апрель 4, 2025

The necrotrophic fungal phytopathogen, Sclerotinia sclerotiorum (Lib.) de Bary, employs a multilayered strategy to infect wide range of host plants. current study proposed the diamine L -ornithine, non-proteinogenic amino acid that promotes synthesis other essential acids, as an alternative management boost molecular, physiological, and biochemical responses common bean ( Phaseolus vulgaris L.) against white mold disease caused by S. . In vitro experiments showed -ornithine significantly inhibited mycelial growth in dose-dependent manner. Moreover, it markedly diminished severity under greenhouse conditions. stimulated treated plants suggesting tested concentration has no phytotoxicity on Additionally, enhanced non-enzymatic antioxidants (total soluble phenolics flavonoids), enzymatic (CAT, POX, PPO), upregulated gene expression three antioxidant-associated genes PvCAT1 , PvSOD PvGR ). silico analysis genome possesses putative oxaloacetate acetylhydrolase SsOAH ) protein is highly similar its functional analysis, conserved domains, topology with OAH from Aspergillus fijiensis AfOAH Penicillium lagena PlOAH Interestingly, addition potato dextrose broth (PDB) medium down-regulated mycelium Likewise, exogenous application mycelia collected Finally, secretion oxalic PDB well infected leaves. Collectively, plays pivotal role maintaining redox status, boosting defense provides insights may lead innovative eco-friendly approaches for controlling mitigating impact cultivation particularly, crops general.

Язык: Английский

Plant’s cellular response to biotic stress: A review

Physiological and Molecular Plant Pathology, Год журнала: 2025, Номер unknown, С. 102718 - 102718

Опубликована: Май 1, 2025

Язык: Английский