Evaluation of mesenchymal stem cells as an in vitro model for inherited retinal diseases DOI Creative Commons

Maria S. Dodina,

Dzerassa Gurtsieva, Alexander Karabelsky

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Ноя. 15, 2024

Retinal pathologies are major causes of vision impairment and blindness in humans, inherited retinal diseases (IRDs), such as retinitis pigmentosa, Leber congenital amaurosis, Stargardt disease, greatly contribute to this problem. In vitro disease modeling can be used for understanding the development pathology screening therapeutic pharmaceutical compounds. preclinical research phase, models complement vivo by reducing animal studies, decreasing costs, shortening timelines. Additionally, may not always accurately replicate human phenotype. This review examines types cells that create IRD models, including retina-specific cell lines, primary cells, induced pluripotent stem (iPSCs), more. Special attention is given mesenchymal (MSCs), which characterized various isolation sources, relative ease isolation, straightforward differentiation. MSCs derived from bone marrow (BM), adipose tissue (AT), dental (DT), umbilical cord (UC), other sources differentiate into photoreceptor pigment epithelial (RPE) dysfunction most commonly associated with IRDs. Subsequent differentiation carried out via methods: culturing induction media supplemented certain growth factors, co-culturing or their conditioned media, regulating gene expression viral vector-delivered transcription factors (TFs) microRNAs (miRNAs). Compared popular iPSCs, example, MSC-based significantly cheaper faster obtain, making them more feasible large-scale drug screening. Nevertheless, existing methods need further optimization promising platform receive success it deserves.

Язык: Английский

Advancements and Challenges in Stem Cell Transplantation for Regenerative Medicine DOI Creative Commons

Lingxi Wei,

Wenqi Yan,

Wahid Shah

и другие.

Heliyon, Год журнала: 2024, Номер 10(16), С. e35836 - e35836

Опубликована: Авг. 1, 2024

Stem cell transplantation has emerged as a promising avenue in regenerative medicine, potentially facilitating tissue repair degenerative diseases and injuries. This review comprehensively examines recent developments challenges stem transplantation. It explores the identification isolation of various types, including embryonic, induced pluripotent, adult cells derived from multiple sources. Additionally, highlights tissue-specific applications these cells, focusing on bone cartilage regeneration, treatment neurological disorders, management hematological conditions. Future advancements effective resolution current will be crucial fully realizing potential medicine. With responsible ethical practices, field can transform disease injury treatment, ultimately improving quality life for countless individuals.

Язык: Английский

Процитировано

12
Organoid models: applications and research advances in colorectal cancer DOI Creative Commons

Yijie Wu,

Sha Yu,

Xingpo Guo

и другие.

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Фев. 7, 2025

This review summarizes the applications and research progress of organoid models in colorectal cancer research. First, high incidence mortality rates are introduced, emphasizing importance organoids as a model. Second, this provides detailed introduction to concept, biological properties, organoids, including their strengths mimicking structural functional aspects organs. article further analyzes adult stem cell-derived pluripotent discusses advancements for basic research, drug development, personalized treatment evaluation prediction, regenerative medicine. Finally, prospects applying technology its significant value improving patient survival rates. In conclusion, systematically explains highlighting tremendous potential promising

Язык: Английский

Процитировано

2
Preparation and characterization of multilayered microcapsules of Lactobacillus rhamnosus encapsulated with sodium alginate, hyaluronic acid and carrageenan and their protective effects on the retina DOI
Xiaodong Wang, Wenqing Lv, Chunxue Zhai

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141104 - 141104

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Revealing the neurodevelopmental toxicity of face mask-derived microplastics to humans based on neural organoids DOI
Minghui Li, Xue Gao,

Yingying Lan

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер unknown, С. 138084 - 138084

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
CRB1 mutations cause structural and molecular defects in patient-derived retinal pigment epithelium cells DOI
Yini Wang, Yuqin Liang,

Yalan Zhou

и другие.

Experimental Eye Research, Год журнала: 2025, Номер 257, С. 110445 - 110445

Опубликована: Май 22, 2025

Язык: Английский

Процитировано

0
Exploiting induced pluripotent stem cell-derived retinal pigment epithelium to unravel host-pathogen interaction in ocular tuberculosis: a reverse translational in vitro model DOI Creative Commons
Ikhwanuliman Putera,

Sanne van de Meerendonk,

Nicole M. A. Nagtzaam

и другие.

Frontiers in Ophthalmology, Год журнала: 2025, Номер 5

Опубликована: Июнь 4, 2025

Mycobacterium tuberculosis ( Mtb ) can infect the retinal pigment epithelium (RPE) cells. Current in vitro research models for ocular (OTB) only rely on RPE cell culture approaches. Until now it remains unclear why a minority of patients with active systemic (TB) develops concurrent OTB. There is significant variation clinical manifestations OTB, which potentially influenced by ethnic differences and diversity mycobacterial strains. To better understand immunopathobiology particularly an individual’s susceptibility to -infection specific host response, systems utilizing induced pluripotent stem cells (iPSC)-derived offer promising model mimic disease. With this technology, be generated from interest, enabling test hypotheses bench bedside or reverse manner. In current study, we explore utility iPSC-derived as Such approach would overcome drawbacks associated currently commonly used “general” lines disease model. The application offers options identification novel biomarkers study individualized drug screening methods host-directed therapy order restore maintain vision OTB sight-threatening

Язык: Английский

Процитировано

0
The application of retinal organoids in ophthalmic regenerative medicine: A mini-review DOI Creative Commons

Xinmei Lan,

Huixia Jiang,

Qian Wang

и другие.

Regenerative Therapy, Год журнала: 2024, Номер 26, С. 382 - 386

Опубликована: Июнь 1, 2024

Retinal organoids are three-dimensional (3D) microscopic tissues that induced and differentiated from stem cells or progenitor in vitro have a highly similar structure to the retina. With optimization development of 3D retinal culture system improvement differentiation technology, broad application prospects development, regenerative medicine, biomaterial evaluation, disease mechanism investigation, drug screening. In this review we summarize recent their applications ophthalmic medicine. particular, highlight promise challenges use modeling discovery.

Язык: Английский

Процитировано

2
Genetic and Cellular Basis of Impaired Phagocytosis and Photoreceptor Degeneration in CLN3 Disease DOI Creative Commons
Jimin Han,

Sueanne Chear,

Jana Talbot

и другие.

Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(13), С. 23 - 23

Опубликована: Ноя. 13, 2024

Purpose: CLN3 Batten disease (also known as juvenile neuronal ceroid lipofuscinosis) is a lysosomal storage disorder that typically initiates with retinal degeneration but followed by seizure onset, motor decline and premature death. Patient-derived induced pluripotent stem cell–RPE cells show defective phagocytosis of photoreceptor outer segment (POS). Because modifier genes are implicated in disease, our goal here was to investigate direct link between mutation POS defect. Methods: Isogenic control mutant cell lines were generated CRISPR-Cas9-mediated biallelic deletion exons 7 8. A transgenic CLN3Δ7–8/Δ7–8 (CLN3) Yucatan miniswine also used study the impact on phagocytosis. cultured RPE analyzed Western blotting immunohistochemistry. Electroretinogram, optical coherence tomography histological analysis wild-type eyes carried out at 6, 36, or 48 months age. Results: (CLN3 RPE) displayed decreased binding consequently uptake compared isogenic cells. Furthermore, phagocytosed less efficiently than POS. Consistent phagocytosis, lipofuscin/autofluorescence 36 age almost complete loss photoreceptors Conclusions: CLN3Δ7 – 8/ Δ7 8 (which affects ≤85% patients) both leads disease. primary dysfunction independently contribute impaired

Язык: Английский

Процитировано

1
Inherited Retinal Degenerations and Non-Neovascular Age-Related Macular Degeneration: Progress and Unmet Needs DOI Creative Commons
John S. Duncan, Angela Bowman,

Amy Laster

и другие.

Translational Vision Science & Technology, Год журнала: 2024, Номер 13(12), С. 28 - 28

Опубликована: Дек. 17, 2024

Inherited retinal degeneration (IRD) disease and age-related macular (AMD) are leading causes of irreversible vision loss blindness. Although significant progress has advanced the field in past 5 years, challenges remain. The current article reviews accomplishments research advances that have fueled development treatments for patients with IRD AMD, including first approved gene-augmentation treatment RPE65-related complement inhibition therapies to slow progression geographic atrophy (GA) AMD. outlines opportunities address gaps unmet needs should lead additional toward IRDs non-neovascular AMD future.

Язык: Английский

Процитировано

1
Genetic and cellular basis of impaired phagocytosis and photoreceptor degeneration in CLN3 disease DOI Open Access
Jimin Han,

Sueanne Chear,

Jana Talbot

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 9, 2024

Abstract Purpose CLN3 Batten disease (also known as Juvenile Neuronal Ceroid Lipofuscinosis; JNCL) is a lysosomal storage disorder that typically initiates with retinal degeneration but followed by seizure onset, motor decline and premature death. Patient-derived iPSC-RPE cells show defective phagocytosis of photoreceptor outer segments (POSs). Because modifier genes are implicated in disease, our goal here was to investigate direct link between mutation POS defect. Methods Isogenic control mutant stem cell lines were generated CRISPR-Cas9­mediated biallelic deletion exons 7 8. A transgenic Δ 7-8/ 7-8 ( ) Yucatan miniswine also used study the impact on phagocytosis. cultured RPE analyzed Western blotting immunohistochemistry. Electroretinogram, optical coherence tomography histological analysis 7/8 wild-type eyes carried out at 6-, 36-, or 48-month age. Results RPE) displayed reduced binding consequently decreased uptake compared isogenic cells. Furthermore, phagocytosed less efficiently than POS. Consistent phagocytosis, lipofuscin/autofluorescence 36 months-of-age almost complete loss photoreceptors 48 months Conclusions (that affects up 85% patients) both POSs leads disease. primary dysfunction independently contribute impaired

Язык: Английский

Процитировано

0