Chemosensors,
Год журнала:
2025,
Номер
13(1), С. 26 - 26
Опубликована: Янв. 20, 2025
Cardiac
troponin
I
(cTnI)
is
a
crucial
biomarker
for
the
early
detection
of
acute
myocardial
infarction
(AMI),
playing
significant
role
in
cardiac
health
assessment.
Molecularly
imprinted
polymers
(MIPs)
are
valued
their
stability,
ease
fabrication,
reusability,
and
selectivity.
However,
using
analyte
as
template
can
be
costly,
especially
if
expensive.
In
such
cases,
dummy
(DT)
with
similar
chemico-physical
properties
useful.
This
study
aimed
to
design
DT-MIP
cTnI
cytochrome
c
(Cyt
c)
template,
combining
computational
experimental
approaches.
Molecular
docking
identified
binding
sites
on
Cyt
poly(o-phenylenediamine)
(5PoPD)
pentamers.
Interactions
energies
were
examined
all-atom
molecular
dynamics
(MDs)
simulations
structural
interaction
fingerprint
(SIFt)
calculations.
A
DT-MIP-modified
electrode
was
prepared
by
electropolymerizing
o-PD
presence
template.
Electrochemical
techniques
monitored
electropolymerization,
removal,
target
analyte.
The
results
showed
that
DT-MIPs
exhibited
high
affinity
cTnI,
consistent
observed
MD
simulations.
satisfactory
correlation
between
validated
our
model-based
approach
rational
molecularly
polymers.
LWT,
Год журнала:
2024,
Номер
206, С. 116567 - 116567
Опубликована: Июль 29, 2024
Rapid
and
accurate
detection
of
pathogenic
bacteria
is
crucial
for
ensuring
food
safety
public
health.
In
this
study,
we
aimed
to
develop
a
novel
molecularly
imprinted
polymer
sensor
based
on
screen-printed
electrodes
the
specific
Salmonella
typhimurium.
The
was
constructed
by
electropolymerizing
dopamine
in
presence
typhimurium
electrode
surface,
followed
removal
create
binding
cavities.
demonstrated
excellent
specificity
typhimurium,
with
limit
approximately
101
CFU/ml
time
only
4
min,
accurately
differentiating
from
other
common
foodborne
pathogens
such
as
Escherichia
coli
Listeria
monocytogenes.
performance
validated
using
real
samples,
including
pork
milk,
showing
its
well
suited
rapid
on-site
bacteria.
development
electrochemical
represents
significant
advancement
field
biosensors,
offering
promising
tool
monitoring
health
protection.
Chemosensors,
Год журнала:
2025,
Номер
13(1), С. 26 - 26
Опубликована: Янв. 20, 2025
Cardiac
troponin
I
(cTnI)
is
a
crucial
biomarker
for
the
early
detection
of
acute
myocardial
infarction
(AMI),
playing
significant
role
in
cardiac
health
assessment.
Molecularly
imprinted
polymers
(MIPs)
are
valued
their
stability,
ease
fabrication,
reusability,
and
selectivity.
However,
using
analyte
as
template
can
be
costly,
especially
if
expensive.
In
such
cases,
dummy
(DT)
with
similar
chemico-physical
properties
useful.
This
study
aimed
to
design
DT-MIP
cTnI
cytochrome
c
(Cyt
c)
template,
combining
computational
experimental
approaches.
Molecular
docking
identified
binding
sites
on
Cyt
poly(o-phenylenediamine)
(5PoPD)
pentamers.
Interactions
energies
were
examined
all-atom
molecular
dynamics
(MDs)
simulations
structural
interaction
fingerprint
(SIFt)
calculations.
A
DT-MIP-modified
electrode
was
prepared
by
electropolymerizing
o-PD
presence
template.
Electrochemical
techniques
monitored
electropolymerization,
removal,
target
analyte.
The
results
showed
that
DT-MIPs
exhibited
high
affinity
cTnI,
consistent
observed
MD
simulations.
satisfactory
correlation
between
validated
our
model-based
approach
rational
molecularly
polymers.
