Identification of Novel miRNA Biomarkers for Epilepsy: A qRT-PCR-Based Profiling Study
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
Abstract
Epilepsy
is
a
widespread
neurological
disorder
affecting
over
70
million
individuals
globally,
with
approximately
4.9
new
cases
diagnosed
annually.
Despite
extensive
research,
the
underlying
mechanisms
of
epilepsy
remain
incompletely
elucidated.
MicroRNAs
(miRNAs)
are
being
increasingly
utilized
as
crucial
biomarkers
in
various
disorders,
and
their
targeting
proteins
involved
disease
pathogenesis
represents
promising
therapeutic
strategy.
This
study
aimed
to
identify
novel
candidate
miRNAs
associated
evaluate
diagnostic
potential.
The
expression
profiles
84
were
analyzed
plasma
samples
from
20
patients
25
controls
using
qRT-PCR
on
BioMarkTM
96.96
Dynamic
Array
(Fluidigm
Corporation)
system.
In
group,
total
44
exhibited
decreased
compared
control
which
24
statistically
significant.
Notably,
18
demonstrated
highly
significant
reduction
levels
(
p<
0.0001).
These
include
hsa-let-7e-5p,
hsa-miR-105-5p,
hsa-miR-106b-5p,
hsa-miR-107,
hsa-miR-125b-5p,
hsa-miR-128,
hsa-miR-135b-5p,
hsa-miR-138-5p,
hsa-miR-152,
hsa-miR-15a-5p,
hsa-miR-193b-3p,
hsa-miR-203a,
hsa-miR-339-5p,
hsa-miR-381-3p,
hsa-miR-409-3p,
hsa-miR-511,
hsa-miR-539-5p,
hsa-miR-7-5p.
40
increased
among
28
23
increase
(
p
<
hsa-miR-101-3p,
hsa-miR-130a-3p,
hsa-miR-132-3p,
hsa-miR-146a-5p,
hsa-miR-146b-5p,
hsa-miR-181a-5p,
hsa-miR-212-3p,
hsa-miR-27a-3p,
hsa-miR-28-5p,
hsa-miR-302b-5p,
hsa-miR-337-3p,
hsa-miR-338-3p,
hsa-miR-34a-5p,
hsa-miR-455-5p,
hsa-miR-485-5p,
hsa-miR-487a,
hsa-miR-499a-5p,
hsa-miR-509-3p,
hsa-miR-512-3p,
hsa-miR-518b,
hsa-miR-652-3p,
hsa-miR-9-3p,
hsa-miR-95.
findings
suggest
that
hold
potential
innovative,
non-invasive
for
early
detection
diagnosis
epilepsy,
offering
avenue
advancing
clinical
practices.
Язык: Английский
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