MYCN/MNX1 Axis Drives NSCLC Progression by Inducing Macrophage M2 Polarization and CD8+ T Cell Apoptosis via the Wnt/β‐Catenin Pathway DOI

Chengzhang Cao,

Hong-Toan Lai,

Shi Yu-zhen

и другие.

Journal of Biochemical and Molecular Toxicology, Год журнала: 2025, Номер 39(4)

Опубликована: Апрель 1, 2025

ABSTRACT Enhanced macrophage M2 polarization and CD8 + T cell dysfunction contribute to the pathophysiology of non‐small lung cancer (NSCLC). Motor neuron pancreatic homeobox 1 (MNX1) has emerged as a potential tumor‐promoting player. Here, we clarified activity MNX1 in NSCLC. PMA‐induced THP‐1 M0‐like macrophages or cells were co‐cultured with NSCLC cells. Cell colony formation, migration, proliferation, apoptosis, invasiveness assessed by wound healing, CCK‐8, flow cytometry, transwell assays, respectively. The ratio CD206 was analyzed cytometry. Ki‐67 expression tested immunofluorescence. ChIP luciferase assays used evaluate relationship between MYCN MNX1. highly expressed NSCLC, its loss‐of‐function suppressed growth, motility, depletion also diminished apoptosis. Mechanistically, increased at transcriptional level. increase reversed impact on malignant behaviors, polarization, viability. vivo growth A549 subcutaneous xenografts. Additionally, counteracted Wnt/β‐catenin pathway. Our findings elucidate oncogenic role MYCN/MNX1/Wnt/β‐catenin pathway driving diminishing study thus uncovers novel mechanism underlying development highlights targets for combating

Язык: Английский

MALAT1: A key regulator in lung cancer pathogenesis and therapeutic targeting DOI
Asif Ahmad Bhat, Obaid Afzal, Muhammad Afzal

и другие.

Pathology - Research and Practice, Год журнала: 2023, Номер 253, С. 154991 - 154991

Опубликована: Ноя. 30, 2023

Язык: Английский

Процитировано

57

Exploring the role of lncrna neat1 knockdown in regulating apoptosis across multiple cancer types: A review DOI
Md Sadique Hussain, Gaurav Gupta, Muhammad Afzal

и другие.

Pathology - Research and Practice, Год журнала: 2023, Номер 252, С. 154908 - 154908

Опубликована: Окт. 31, 2023

Язык: Английский

Процитировано

54

Kaempferol: Paving the path for advanced treatments in aging-related diseases DOI Creative Commons
Md Sadique Hussain, Abdulmalik Saleh Alfawaz Altamimi, Muhammad Afzal

и другие.

Experimental Gerontology, Год журнала: 2024, Номер 188, С. 112389 - 112389

Опубликована: Март 8, 2024

Aging-related diseases (ARDs) are a major global health concern, and the development of effective therapies is urgently needed. Kaempferol, flavonoid found in several plants, has emerged as promising candidate for ameliorating ARDs. This comprehensive review examines Kaempferol's chemical properties, safety profile, pharmacokinetics, highlights its potential therapeutic utility against underpinned by distinctive structure, which confers antioxidative anti-inflammatory properties. Kaempferol counteracts reactive oxygen species (ROS) modulates crucial cellular pathways, thereby combating oxidative stress inflammation, hallmarks low toxicity wide margins, demonstrated preclinical clinical studies, further substantiate potential. Compelling evidence supports substantial addressing ARDs through mechanisms, notably anti-inflammatory, antioxidant, anti-apoptotic actions. exhibits versatile neuroprotective effect modulating various proinflammatory signaling including NF-kB, p38MAPK, AKT, β-catenin cascade. Additionally, it hinders formation aggregation beta-amyloid protein regulates brain-derived neurotrophic factors. In terms anticancer potential, kaempferol acts diverse inducing apoptosis, arresting cell cycle at G2/M phase, suppressing epithelial-mesenchymal transition (EMT)-related markers, affecting phosphoinositide 3-kinase/protein kinase B pathways. Subsequent studies should focus on refining dosage regimens, exploring innovative delivery systems, conducting trials to translate these findings into applications.

Язык: Английский

Процитировано

48

Unraveling NEAT1's complex role in lung cancer biology: a comprehensive review. DOI
Md Sadique Hussain, Obaid Afzal, Gaurav Gupta

и другие.

PubMed, Год журнала: 2024, Номер 23, С. 34 - 52

Опубликована: Янв. 1, 2024

This review delves into the pivotal role of long non-coding RNA NEAT1 in cancer biology, particularly lung (LC). It emphasizes NEAT1's unique subcellular localization and active involvement gene regulation chromatin remodeling. The highlights impact on LC development progression, including cell processes such as proliferation, migration, invasion, resistance to therapy, positioning it a potential diagnostic marker therapeutic target. complex web regulatory interactions with proteins microRNAs is explored, alongside challenges targeting therapeutically. concludes optimistically, suggesting future avenues for research personalized therapies, shedding light crucial LC. See also Graphical abstract(Fig. 1).

Язык: Английский

Процитировано

30

MicroRNA-21's role in PTEN suppression and PI3K/AKT activation: Implications for cancer biology DOI
Himmat Singh Chawra, Mohit Agarwal, Anurag Mishra

и другие.

Pathology - Research and Practice, Год журнала: 2024, Номер 254, С. 155091 - 155091

Опубликована: Янв. 3, 2024

Язык: Английский

Процитировано

19

Osteomyelitis and non-coding RNAS: A new dimension in disease understanding DOI
Md Sadique Hussain, Nusrat K. Shaikh, Mohit Agrawal

и другие.

Pathology - Research and Practice, Год журнала: 2024, Номер 255, С. 155186 - 155186

Опубликована: Фев. 2, 2024

Язык: Английский

Процитировано

18

Autophagy‐associated non‐coding RNAs: Unraveling their impact on Parkinson's disease pathogenesis DOI Creative Commons
Md Sadique Hussain, Ehssan Moglad, Muhammad Afzal

и другие.

CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(5)

Опубликована: Май 1, 2024

Parkinson's disease (PD) is a degenerative neurological condition marked by the gradual loss of dopaminergic neurons in substantia nigra pars compacta. The precise etiology PD remains unclear, but emerging evidence suggests significant role for disrupted autophagy-a crucial cellular process maintaining protein and organelle integrity.

Язык: Английский

Процитировано

18

From carcinogenesis to therapeutic avenues: lncRNAs and mTOR crosstalk in lung cancer DOI
Md Sadique Hussain, Abdulmalik Saleh Alfawaz Altamimi, Muhammad Afzal

и другие.

Pathology - Research and Practice, Год журнала: 2023, Номер 253, С. 155015 - 155015

Опубликована: Дек. 6, 2023

Язык: Английский

Процитировано

25

Exosomal ncRNAs in liquid biopsies for lung cancer DOI
Md Sadique Hussain, Gaurav Gupta, Nehmat Ghaboura

и другие.

Clinica Chimica Acta, Год журнала: 2024, Номер 565, С. 119983 - 119983

Опубликована: Окт. 3, 2024

Язык: Английский

Процитировано

15

Circular RNAs in the KRAS pathway: Emerging players in cancer progression DOI
Md Sadique Hussain, Ehssan Moglad, Muhammad Afzal

и другие.

Pathology - Research and Practice, Год журнала: 2024, Номер 256, С. 155259 - 155259

Опубликована: Март 11, 2024

Язык: Английский

Процитировано

12