Protection of Broiler Chickens Against Necrotic Enteritis by Intrapulmonary Delivery of a Live Clostridium perfringens Vaccine Exploiting the Gut-Lung–Axis Concept

Avian Diseases, Год журнала: 2024, Номер 68(3)

Опубликована: Авг. 21, 2024

(CP)-induced necrotic enteritis (NE) is an economically important disease in the broiler chicken industry. The incidence of NE common 3-to-6-wk-old chickens, once maternal antibodies start declining. Developing effective vaccination strategy against NE, preferably delivering a single dose vaccine at hatch to protect chickens without booster vaccine, enormous challenge. objective this study was induce mucosal immunity intestines by intrapulmonary (IPL) delivery live CP hatch, exploiting gut-lung-axis (GLA) concept following

Язык: Английский

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Effects of challenge with Clostridium perfringens, Eimeria and both on ileal microbiota of yellow feather broilers

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Дек. 1, 2022

In the poultry industry worldwide, Clostridium perfringens has been causing major economic loss as it can cause necrotic enteritis (NE). The coccidial infection considered most important predisposing factor of NE caused by C. perfringens. this study, we aimed to advance our knowledge on ileal microbiota yellow feather broilers under and/or Eimeria challenge. Total 80 healthy day old were randomly assigned four groups including: Control, challenge group (C. Per), (Cocc), and plus (Comb). On 14, Cocc Comb orally gavaged 1 ml PBS solution containing 25,000 oocysts brunetti maxima. Starting 17, Per 10 mL per bird (4 × 107 CFU/mL, ATCC® 13124™ Strain) every for 6 days. 16S rRNA gene sequencing was performed extracted DNA digesta samples. results showed that alone did not affect alpha diversity microbiome in but co-infection with significantly decreased microbiota. also relative abundance beneficial bacteria including Bacteroidetes at phylum level Faecalibacterium genus level. At species level, Candidatus Arthromitus challenged groups. This microbial shift information Perfringens provide reference data development therapeutic approaches yellow-feather broiler chickens.

Язык: Английский

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Immune Response Evaluation in Commercial Turkeys Affected with Clostridial Dermatitis

Avian Diseases, Год журнала: 2023, Номер 67(1)

Опубликована: Март 15, 2023

Evaluación de la respuesta inmune en pavos comerciales afectados por dermatitis clostridial. La clostridial (CD), causada Clostridium septicum y perfringens, es una enfermedad emergente económicamente importante los caracterizada muerte súbitas necrótica. Se conoce poco acerca las respuestas inmunitarias En el presente estudio, se aisló C. durante un brote reciente, tejidos (piel, músculo bazo) recolectaron analizaron para determinar expresión genes inmunitarios junto con muestras aves clínicamente sanas. Los resultados mostraron que tenían niveles significativamente más altos transcritos IL-1β, IL-6, IFNγ, and iNOS piel, músculos bazo comparación también transcripción elevada del gene receptor tipo toll (TLR21) piel bazo, lo sugiere papel este reconocimiento inmunitario. IL-4 e IL-13 fue mayor afectadas. Aves adicionales mismas granjas afectadas sanas fueron examinadas serología revelaron anticuerpos séricos IgM IgY. Además, estimulación in vitro macrófagos MQ-NCSU condujo a regulación transcripcional al alza IL-1β mientras IL-10 reguló baja. superficial proteína MHC-II producción celular óxido nítrico aumentaron estimulados septicum, indica activación celular. Colectivamente, estos hallazgos sugieren huésped implican inflamatoria robusta, así como mediada citoquinas IL4/IL-13 pueden ayudar inmunidad anticuerpos.

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Buffered formic acid and a monoglyceride blend improve performance and modulate gut bacteria and immunity gene expression in broilers under necrotic enteritis challenge

Poultry Science, Год журнала: 2023, Номер 102(10), С. 102978 - 102978

Опубликована: Авг. 2, 2023

Due to the removal of antibiotics from animal feed, alternatives have been sought control necrotic enteritis (NE) in broilers. The current study investigated effects buffered formic acid (Amasil NA) and monoglycerides short- medium-chain fatty acids (Balangut LS P) on performance gut health broilers challenged with NE. A total 816 as-hatched 1-d-old chicks (Cobb 500) were randomly assigned 6 treatments 8 replicates. Treatments were: T1) nonchallenged control; T2) NE T3) Amasil NA (challenge plus NA, 0.3% throughout all phases); T4) Balangut P P, 0.5%, 0.3%, 0.2% starter, grower finisher phases, respectively; T5) Combined combination T3 T4); T6) Antibiotic Zn bacitracin, 0.05 % phases). Birds orally gavaged live Eimeria vaccine species (d 9) Clostridium perfringens 14 15). On d 16, birds sampled evaluate permeability, microbiota, mRNA abundance jejunum. data analyzed JMP software using one-way ANOVA Tukey's test separate means, Kruskal-Wallis was used for non-normally-distributed parameters. Results showed that decreased (P<0.05) feed conversion ratio compared ones at end study. reduced (P < 0.05) level cecal Bacteriods group, whereas shifted levels ileal Bifidobacteria, Enterobacteriaceae, Lactobacillus towards > 0.05). challenge upregulated 0.001) expression IL-21R, zeta chain T cell receptor (ZAP70), dual specificity phosphatase 4 (DUSP4) birds, an intermediate pattern these genes antibiotic groups. In conclusion, has potential be as additive improve broiler chickens, especially under challenging conditions such infections.

Язык: Английский

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Mucosal and systemic lymphoid immune responses against Clostridium perfringens strains with variable virulence in the production of necrotic enteritis in broiler chickens

Avian Pathology, Год журнала: 2022, Номер 52(2), С. 108 - 118

Опубликована: Дек. 1, 2022

Necrotic enteritis (NE), caused by Clostridium perfringens, is an economically important disease of chickens. Although NE pathogenesis moderately well studied, the host immune responses against C. perfringens are poorly understood. The present study used experimental model to characterize lymphoid in caecal tonsils (CT), bursa Fabricius, Harderian gland (HG) and spleen tissues broiler chickens infected with four netB+ strains (CP1, CP5, CP18, CP26), which CP18 CP26 also carried tpeL gene. gross histopathological lesions revealed CP5 be avirulent, while CP1, were virulent being "very virulent". Gene expression analysis showed that, induced a significantly upregulated pro-inflammatory IL-1β gene CT, CP26-infected birds had higher CT transcription IFNγ IL-6 genes compared CP5-infected or uninfected Furthermore, infection led increased bursal HG anti-inflammatory/regulatory genes, IL-10 TGFβ, control, CP1 groups. Additionally, splenic pro- anti-inflammatory transcriptional changes observed only An antibody-mediated response, as characterized IL-4 and/or IL-13 elevated IgM levels strains, particularly group controls, was evident. Collectively, our findings suggest that during spatially regulated such inflammatory depend on virulence strain.

Язык: Английский

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