Growth patterns and heat tolerance analysis of dwarf chicken with frizzled feather

Poultry Science, Год журнала: 2025, Номер 104(5), С. 104996 - 104996

Опубликована: Март 6, 2025

Chickens are covered with feathers, lack sweat glands, and sensitive to the thermal environment. Previously, our group bred a novel dwarf chicken strain frizzled feather, named as feather (DFC). The cumulative growth of body weight size were analyzed 3 mathematical models. Subsequently, chickens grouped investigate impact heat stress (HS) on their slaughter performance, histomorphological development gene mRNA change (HSP70, muscle appetite-related factors) using quantitative real-time PCR, tissue sections Western Blot. In HS group, placed at 34 ± 1°C for 8 hours (9:00 am - 17:00 pm) day lasted 2 weeks, while in control fed 26 1°C. Chicken samples collected age 120 days evaluate production histological changes, expression changes. Our results found that Gompertz model was best fitting DFC. integrity muscle, liver, spleen, small intestine tissues affected under conditions. Correspondingly, length ileum significantly decreased (P < 0.05), thigh factor MYOD1 down-regulated MSTN up-regulated 0.001). addition, jejunum VH / CD reduced 0.05). appetite-promoting factors AMPKα-1 AGRP gut-brain axis appetite-restrain CCK, GHRL, CART 0.05 or P 0.01). Moreover, intestinal transport absorption ZO1, OCLN, PepT1, SGLT1, CAT1 0.01), GLUT1 These indicated mainly impacted appetite did not disrupt nutrient function these chickens. DFC appeared be more tolerant hot environments size, low basal metabolic rate.

Язык: Английский

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Liver transcriptome response to avian pathogenic Escherichia coli infection in broilers with corticosterone treatment

Poultry Science, Год журнала: 2025, Номер 104(5), С. 105020 - 105020

Опубликована: Март 11, 2025

Avian pathogenic Escherichia coli (APEC) infection has high morbidity and mortality, multiple stressors encountered during rearing place poultry in a state of stress. However, research on how cope with APEC under stress situation is still limited. In this study, we established broiler model by corticosterone (CORT) administration subcutaneously for 7 consecutive days, followed challenge intramuscularly. CORT treatment significantly reduced body weight (BW) average daily gain (ADG) while increasing feed conversion ratio (FCR) (P < 0.01). decreased ADG elevated plasma heterophil to lymphocyte (H/L) 0.05). Additionally, aspartate aminotransferase (AST), AST alanine (AST/ALT) ratio, lactate dehydrogenase (LDH) levels increased Histopathological analysis revealed structural damage liver, indicating that induced liver injury. pretreatment broilers exhibited milder histopathological lesions lower AST, ALT, LDH 0.05) compared alone. lysozyme (LZM), total protein (TP), globulin (GLOB) 0.05), further elevating their concentrations alone, suggesting an enhanced innate immune response. Liver transcriptomic identified 768, 335, 567 differentially expressed genes (DEGs) following CORT, APEC, or both treatments, respectively, enriched cytokine-cytokine receptor interaction, PPAR signaling pathway, Toll-like MAPK steroid hormone biosynthesis arachidonic acid metabolism, phagosome etc., regulates lipid metabolism immunity, induces inflammation disrupts metabolism. Notably, may mitigate injury enhancing function. Moreover, glucocorticoid (GR) regulate DEGs expressions, thus affected response treatments. These results suggest treatment, infection, affect the growth performance, function broilers, play crucial role.

Язык: Английский

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Major Oxidative and Antioxidant Mechanisms During Heat Stress-Induced Oxidative Stress in Chickens

Antioxidants, Год журнала: 2025, Номер 14(4), С. 471 - 471

Опубликована: Апрель 15, 2025

Heat stress (HS) is one of the most important stressors in chickens, and its adverse effects are primarily caused by disturbing redox homeostasis. An increase electron leakage from mitochondrial transport chain major source free radical production under HS, which triggers other enzymatic systems to generate more radicals. As a defense mechanism, cells have non-enzymatic antioxidant that work cooperatively against The generation radicals, particularly reactive oxygen species (ROS) nitrogen (RNS), HS condition outweighs cellular capacity, resulting oxidative damage macromolecules, including lipids, carbohydrates, proteins, DNA. Understanding these detrimental processes protective mechanisms developing mitigation strategies HS. This review summarizes current understanding their molecular generating or neutralizing ROS/RNS. Importantly, this explores potential lead development heat-stressed highlighting unique behavioral physiological responses thermal stress. Further, we summarize findings associated with chickens.

Язык: Английский

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Biogenic Selenium Nanoparticles Synthesized with Alginate Oligosaccharides Alleviate Heat Stress-Induced Oxidative Damage to Organs in Broilers through Activating Nrf2-Mediated Anti-Oxidation and Anti-Ferroptosis Pathways

Antioxidants, Год журнала: 2023, Номер 12(11), С. 1973 - 1973

Опубликована: Ноя. 6, 2023

Selenium (Se) is an essential trace element for maintaining health due to its ideal antioxidant properties. We previously prepared a new type of biogenic selenium nanoparticles based on alginate oligosaccharides (SeNPs-AOS), and this study aimed investigate the protective effects SeNPs-AOS (Se particle size = 80 nm, Se content 8%) organ in broilers challenged with HS. A total 192 21-day-old Arbor Acres were randomly divided into four groups according 2 × experimental design, including thermoneutral zone group (TN, raised under 23 ± 1.5 °C); TN + (TN supplemented 5 mg/kg SeNPS-AOS); HS (HS, 33 °C 10 h/day); (HS SeNPS-AOS). There six replicates each (eight per replicate). The results showed that improved splenic histomorphology, enhanced activity catalase (CAT) glutathione peroxidase (GSH-Px) spleen, as well upregulating mRNA expression antioxidant-related genes In addition, reversed pathological changes bursa caused by increased GST, GSH-Px, CAT upregulated Nrf2 heat-stressed broilers. dietary hepatic damage, GSH-Px liver, while downregulating Keap1 gene liver during Moreover, anti-ferroptosis-related conclusion, could relieve HS-induced oxidative damage Fabricius Nrf2-mediated also upregulate Nrf2-related anti-ferroptosis These findings are beneficial development nano-antioxidants

Язык: Английский

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Nano-Se exhibits limited protective effect against heat stress induced poor breast muscle meat quality of broilers compared with other selenium sources

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 8, 2024

At present, heat stress (HS) has become a key factor that impairs broiler breeding industry, which causes growth restriction and poor meat quality of broilers. Selenium (Se) is an excellent antioxidant plays unique role in improvement. Recent years, nano-selenium (NanoSe) received tremendous attention livestock production, due to its characteristic good antibacterial performance vitro. Here, we developed the stressed-broiler model investigate protective effects NanoSe on broilers compare whether there are differences with other Se sources (Sodium selenite, SS; Selenoyeast, SeY; Selenomethionine, SeMet). HS jeopardized caused breast muscle broilers, were accompanied by lowered capacity, increased glycolysis, anaerobic metabolism pyruvate, mitochondrial abnormal tricarboxylic acid (TCA) cycle. All supplementation exhibited effects, concentration promoted expression selenoproteins, improved homeostasis TCA cycle aerobic thus exposed HS. However, unlike three sources, effect stressed-broilers was not ideal, limited impact pH value, drip loss cooking muscle. Compared showed lowest levels slow MyHC, highest fast MyHC glycogen, mRNA glycolysis-related genes (PFKM PKM), protein HSP60 CLPP, enzyme activities GSH-Px, citroyl synthetase (CS) isocitrate dehydrogenase (ICD) Consistent SS, deposition lower than SeY or SeMet. Besides, regulatory efficiency selenoproteins (such as SELENOS) also worse sources. Through comparing quality, deposition, fiber type conversion, homeostasis, cycle-related indicators stressed found organic (SeY SeMet) better inorganic (SS) NanoSe. As new source, though some compared

Язык: Английский

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Effects of sodium lactate injection on meat quality and lactate content in broiler chickens: emphasis on injection method and dosage

Poultry Science, Год журнала: 2024, Номер 103(10), С. 104084 - 104084

Опубликована: Июль 10, 2024

This study aims to develop an experimental model of high lactate levels in broilers mimic the condition birds under stress or diseases and evaluate its consequent effects on meat quality. The injection sites dosage were compared separately 2 experiments. Experiment 1 includes 3 sites: intraperitoneal injection, intramuscular subcutaneous injection. was a experiment based results 1: sodium group with 1.5, 3, 6 mM concentration. showed that injecting intraperitoneally, intramuscularly, subcutaneously all significantly decreased body weight breast muscle while elevating lactic acid both blood broilers. Moreover, methods caused significant reduction pH

Язык: Английский

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Effects of chronic heat stress on Ca2+ homeostasis, apoptosis, and protein carbonylation profiles in the breast muscle of broilers

Poultry Science, Год журнала: 2024, Номер 103(12), С. 104342 - 104342

Опубликована: Сен. 19, 2024

Язык: Английский

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Effects of cardamonin on the growth performance, intestinal barrier function and intestinal microbiota of Danzhou chickens under heat stress

Poultry Science, Год журнала: 2024, Номер 103(12), С. 104362 - 104362

Опубликована: Сен. 26, 2024

Язык: Английский

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