Effects of Oligomeric Proanthocyanidins on Cadmium-Induced Extracellular Matrix Damage via Inhibiting the ERK1/2 Signaling Pathway in Chicken Chondrocytes
Veterinary Sciences,
Год журнала:
2025,
Номер
12(4), С. 317 - 317
Опубликована: Март 31, 2025
Cadmium
(Cd)
is
a
toxic,
non-essential
metal
that
primarily
enters
animal
bodies
through
the
digestive
and
respiratory
systems,
leading
to
damage
multiple
organs
tissues.
Cd
can
accumulate
in
cartilage
induce
chondrocytes.
Procyanidins
(PAs),
also
known
as
concentrated
tannic
acid
or
oligomeric
proanthocyanidins
(OPCs),
exhibit
diverse
biological
pharmacological
activities.
However,
mechanism
of
OPCs
alleviates
Cd-induced
chondrocytes
chickens
remains
be
further
explored
vitro.
Chondrocytes
were
isolated
from
both
ends
tibia
17-day-old
SPF
chicken
embryos,
then
subsequently
treated
with
various
concentrations
(0,
1,
2.5,
5,
10
μmol/L)
10,
20,
40
investigate
underlying
extracellular
matrix
(ECM)
degradation
damage.
reduced
cell
viability,
glycosaminoglycan
(GAG)
secretion,
ECM
by
decreasing
expression
type
II
collagen
alpha
1
(COL2A1)
aggrecan
(ACAN)
while
increasing
release
protein
(COMP),
along
elevated
levels
matrix-degrading
enzymes,
such
metalloproteinases
(MMP1),
MMP10,
MMP13,
disintegrin
metalloproteinase
thrombospondin
motifs
4
(ADAMTS4)
ADAMTS5.
induced
phosphorylation
signal-regulated
kinases
1/2
(ERK1/2)
impairing
synthesis,
an
effect
could
alleviated
ERK1/2
inhibitor
U0126.
5
μmol/L
OPCs,
it
was
found
inhibited
activation
signaling
pathway
promoting
synthesis
alleviating
chickens.
This
study
provides
theoretical
basis
for
clinical
research
on
respect
prevention
treatment
chondrogenic
diseases
poultry.
Язык: Английский
A new perspective in avian bone health: dietary supplementation with a standardized dry grape extract improves pullets’ bones’ quality through metabolic modulation
Poultry Science,
Год журнала:
2025,
Номер
unknown, С. 105270 - 105270
Опубликована: Май 1, 2025
In
laying
hens,
maintaining
optimal
bone
health
and
development
from
the
early
stages
is
crucial,
as
it
directly
affects
their
egg-laying
efficiency,
overall
welfare
productivity.
Studies
have
shown
that
grape
polyphenols
Vitis
vinifera
enhance
in
both
humans
animals.
Still,
mechanisms
behind
this
effect
remain
unclear,
given
diversity
of
varying
action.
The
objective
study
was
to
investigate
a
standardized
dry
extract
(SDGE)
on
quality
metabolome
future
hens
reared
commercial
conditions.
Therefore,
36300
day-old
pullets
were
randomly
divided
into
two
barns
same
farm
site.
Both
received
diet,
with
addition
30
mg/kg
SDGE
(Nor-Grape®,
Nor-Feed,
France)
day
1
until
week
17
supplemented
group
(SDGE).
At
end
supplementation
period,
several
parameters
analyzed
50
individuals
per
group.
Additionally,
non-targeted
metabolomics
plasma
bones
performed
uncover
impact
pullet's
metabolome.
Results
demonstrated
keel
deformity
tended
improve
following
(P
=
0.10).
Moreover,
intake
significantly
increased
mineral
content,
compared
control
<
0.05)
increase
calcium
(Ca)
0.074)
phosphorus
(P)
content
0.055).
On
other
hand,
samples
revealed
an
fatty
acids
glycerophospholipid
metabolisms
while
analysis
uncovered
pathways
related
sphingolipid
estrogen
mediated
signaling
pathways.
Overall,
suggests
novel
through
lipid
metabolism
highlights
nutritional
intervention
developmental
stage
improves
prior
entry
phase,
thus
proving
useful
tool
management.
Язык: Английский
Tibial Damage Caused by T-2 Toxin in Goslings: Bone Dysplasia, Poor Bone Quality, Hindered Chondrocyte Differentiation, and Imbalanced Bone Metabolism
Animals,
Год журнала:
2024,
Номер
14(15), С. 2281 - 2281
Опубликована: Авг. 5, 2024
T-2
toxin,
the
most
toxic
type
A
trichothecene,
is
widely
present
in
grain
and
animal
feed,
causing
growth
retardation
tissue
damage
poultry.
Geese
are
more
sensitive
to
toxin
than
chickens
ducks.
Although
has
been
reported
cause
tibial
plate
(TGP)
chondrodysplasia
chickens,
caused
by
geese
not
fully
demonstrated.
This
study
aims
investigate
adverse
effects
of
on
bone
development,
quality,
chondrocyte
differentiation,
metabolism.
Here,
forty-eight
one-day-old
male
Yangzhou
goslings
were
randomly
divided
into
four
groups
daily
gavaged
with
at
concentrations
0,
0.5,
1.0,
2.0
mg/kg
body
weight
for
21
days,
respectively.
The
development
gosling
size
was
determined
weighing
taking
measurements
after
exposure
different
toxin.
Changes
characteristics
radiographic
examination,
phenotypic
measurements,
quality
composition
analyses.
Chondrocyte
differentiation
TGP
metabolism
characterized
cell
morphology,
gene-specific
expression,
serum
marker
levels.
Results
showed
that
treatment
resulted
a
lower
weight,
volume,
length,
middle
width,
circumference
tibia
dose-dependent
manner
(
Язык: Английский
Mining Candidate Genes and Identifying Risk Factors for Leg Disease in Broilers: A Mendelian Randomization Study
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(16), С. 8890 - 8890
Опубликована: Авг. 15, 2024
Clinical
investigations
have
highlighted
disruptions
in
bone
metabolic
processes
and
abnormal
fluctuations
serum
indicator
levels
during
the
onset
of
leg
disease
(LD)
broilers.
However,
presence
a
genetic
causal
relationship
for
this
association
remains
undetermined.
Therefore,
aim
study
is
to
discern
risk
factors
underlying
LD
development
using
1235
sequenced
white-feathered
We
employed
Mendelian
randomization
(MR)
analysis
assess
associations
strength
(BS),
mineral
density
(BMD),
tibial
weight
(TBW),
length
(TBL),
diameter
(TBD),
ash
(BA),
calcium
(Ash
Ca),
phosphorus
P),
(Ca),
(P),
alkaline
phosphatase
(ALP),
osteoprotegerin
(OPG)
with
incidence
LD.
Compelling
evidence
underscores
link
between
developing
decreased
BMD
(odds
ratio
(OR)
=
0.998;
95%
CI:
0.983,
0.993;
P
<
0.001)
narrower
TBD
(OR
0.985,
0.975,
0.994,
0.002).
Additionally,
OPG
concentrations
(OR:
0.995,
0.992,
0.999,
0.008)
were
associated
0.0078,
CI
0.0043
0.0140,
0.001),
indicating
robust
ALP
0.988,
0.984,
0.993,
0.0046,
0.0026,
0.0083,
0.001).
Moreover,
elevated
Ca
0.564,
0.487,
0.655,
0.614,
0.539,
0.699,
TBD.
Elevated
Ca,
P,
ALP,
contribute
disturbances
metabolism,
while
are
greater
This
discovery
elucidates
broilers
could
provide
information
on
LDs,
such
as
osteoporosis,
humans.
Язык: Английский
Phosvitin phosphopeptides and peptides-calcium chelate promote calcium deposition in a three-cell co-culture system by modulating the OPG/RANKL/RANK signaling pathway
Food Bioscience,
Год журнала:
2024,
Номер
62, С. 105065 - 105065
Опубликована: Сен. 6, 2024
Язык: Английский
Oligomeric Proanthocyanidins Ameliorate Cadmium-Induced Senescence of Osteocytes Through Combating Oxidative Stress and Inflammation
Antioxidants,
Год журнала:
2024,
Номер
13(12), С. 1515 - 1515
Опубликована: Дек. 12, 2024
Osteocyte
senescence
is
associated
with
skeletal
dysfunction,
but
how
to
prevent
bone
loss
and
find
the
effective
therapeutic
targets
a
potential
scientific
concern.
Cadmium
(Cd)
widespread
environmental
contaminant
that
causes
substantial
damage
in
both
animals
humans.
Oligomeric
proanthocyanidins
(OPC)
are
naturally
polyphenolic
substances
found
various
plants
demonstrate
significant
anti-senescence
potential.
Here,
we
investigated
protective
effects
of
OPC
against
Cd-induced
osteocytes
identify
regulatory
mechanisms.
alleviated
by
attenuating
cell
cycle
arrest,
reducing
ROS
accumulation,
suppressing
pro-inflammatory
responses
vitro.
Furthermore,
effectively
prevented
breakdown
dendritic
synapses
Correspondingly,
ameliorated
through
activity
vivo.
Taken
together,
our
results
establish
as
promising
agent
ameliorates
osteocyte
mitigating
oxidative
stress
inflammatory
responses.
Язык: Английский