Mining Candidate Genes and Identifying Risk Factors for Leg Disease in Broilers: A Mendelian Randomization Study DOI Open Access

Xinxin Tang,

Peihao Liu, Na Luo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8890 - 8890

Published: Aug. 15, 2024

Clinical investigations have highlighted disruptions in bone metabolic processes and abnormal fluctuations serum indicator levels during the onset of leg disease (LD) broilers. However, presence a genetic causal relationship for this association remains undetermined. Therefore, aim study is to discern risk factors underlying LD development using 1235 sequenced white-feathered We employed Mendelian randomization (MR) analysis assess associations strength (BS), mineral density (BMD), tibial weight (TBW), length (TBL), diameter (TBD), ash (BA), calcium (Ash Ca), phosphorus P), (Ca), (P), alkaline phosphatase (ALP), osteoprotegerin (OPG) with incidence LD. Compelling evidence underscores link between developing decreased BMD (odds ratio (OR) = 0.998; 95% CI: 0.983, 0.993; P < 0.001) narrower TBD (OR 0.985, 0.975, 0.994, 0.002). Additionally, OPG concentrations (OR: 0.995, 0.992, 0.999, 0.008) were associated 0.0078, CI 0.0043 0.0140, 0.001), indicating robust ALP 0.988, 0.984, 0.993, 0.0046, 0.0026, 0.0083, 0.001). Moreover, elevated Ca 0.564, 0.487, 0.655, 0.614, 0.539, 0.699, TBD. Elevated Ca, P, ALP, contribute disturbances metabolism, while are greater This discovery elucidates broilers could provide information on LDs, such as osteoporosis, humans.

Language: Английский

Effects of Oligomeric Proanthocyanidins on Cadmium-Induced Extracellular Matrix Damage via Inhibiting the ERK1/2 Signaling Pathway in Chicken Chondrocytes DOI Creative Commons
Jianhong Gu, Dan Liu,

Anqing Gong

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 317 - 317

Published: March 31, 2025

Cadmium (Cd) is a toxic, non-essential metal that primarily enters animal bodies through the digestive and respiratory systems, leading to damage multiple organs tissues. Cd can accumulate in cartilage induce chondrocytes. Procyanidins (PAs), also known as concentrated tannic acid or oligomeric proanthocyanidins (OPCs), exhibit diverse biological pharmacological activities. However, mechanism of OPCs alleviates Cd-induced chondrocytes chickens remains be further explored vitro. Chondrocytes were isolated from both ends tibia 17-day-old SPF chicken embryos, then subsequently treated with various concentrations (0, 1, 2.5, 5, 10 μmol/L) 10, 20, 40 investigate underlying extracellular matrix (ECM) degradation damage. reduced cell viability, glycosaminoglycan (GAG) secretion, ECM by decreasing expression type II collagen alpha 1 (COL2A1) aggrecan (ACAN) while increasing release protein (COMP), along elevated levels matrix-degrading enzymes, such metalloproteinases (MMP1), MMP10, MMP13, disintegrin metalloproteinase thrombospondin motifs 4 (ADAMTS4) ADAMTS5. induced phosphorylation signal-regulated kinases 1/2 (ERK1/2) impairing synthesis, an effect could alleviated ERK1/2 inhibitor U0126. 5 μmol/L OPCs, it was found inhibited activation signaling pathway promoting synthesis alleviating chickens. This study provides theoretical basis for clinical research on respect prevention treatment chondrogenic diseases poultry.

Language: Английский

Citations

0
A new perspective in avian bone health: dietary supplementation with a standardized dry grape extract improves pullets’ bones’ quality through metabolic modulation DOI Creative Commons

Fatima Zohra ABERKANE,

Paul Engler, Séverine Boisard

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 105270 - 105270

Published: May 1, 2025

Language: Английский

Citations

0
Tibial Damage Caused by T-2 Toxin in Goslings: Bone Dysplasia, Poor Bone Quality, Hindered Chondrocyte Differentiation, and Imbalanced Bone Metabolism DOI Creative Commons
Wang Gu,

Lie Hou,

Qiang Bao

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(15), P. 2281 - 2281

Published: Aug. 5, 2024

T-2 toxin, the most toxic type A trichothecene, is widely present in grain and animal feed, causing growth retardation tissue damage poultry. Geese are more sensitive to toxin than chickens ducks. Although has been reported cause tibial plate (TGP) chondrodysplasia chickens, caused by geese not fully demonstrated. This study aims investigate adverse effects of on bone development, quality, chondrocyte differentiation, metabolism. Here, forty-eight one-day-old male Yangzhou goslings were randomly divided into four groups daily gavaged with at concentrations 0, 0.5, 1.0, 2.0 mg/kg body weight for 21 days, respectively. The development gosling size was determined weighing taking measurements after exposure different toxin. Changes characteristics radiographic examination, phenotypic measurements, quality composition analyses. Chondrocyte differentiation TGP metabolism characterized cell morphology, gene-specific expression, serum marker levels. Results showed that treatment resulted a lower weight, volume, length, middle width, circumference tibia dose-dependent manner (

Language: Английский

Citations

2
Phosvitin phosphopeptides and peptides-calcium chelate promote calcium deposition in a three-cell co-culture system by modulating the OPG/RANKL/RANK signaling pathway DOI
Jiaojiao Chen, Songming Li, Mahmoud Abou-Elsoud

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 62, P. 105065 - 105065

Published: Sept. 6, 2024

Language: Английский

Citations

0
Oligomeric Proanthocyanidins Ameliorate Cadmium-Induced Senescence of Osteocytes Through Combating Oxidative Stress and Inflammation DOI Creative Commons

Gengsheng Yu,

Zehao Wang,

Anqing Gong

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1515 - 1515

Published: Dec. 12, 2024

Osteocyte senescence is associated with skeletal dysfunction, but how to prevent bone loss and find the effective therapeutic targets a potential scientific concern. Cadmium (Cd) widespread environmental contaminant that causes substantial damage in both animals humans. Oligomeric proanthocyanidins (OPC) are naturally polyphenolic substances found various plants demonstrate significant anti-senescence potential. Here, we investigated protective effects of OPC against Cd-induced osteocytes identify regulatory mechanisms. alleviated by attenuating cell cycle arrest, reducing ROS accumulation, suppressing pro-inflammatory responses vitro. Furthermore, effectively prevented breakdown dendritic synapses Correspondingly, ameliorated through activity vivo. Taken together, our results establish as promising agent ameliorates osteocyte mitigating oxidative stress inflammatory responses.

Language: Английский

Citations

0
Mining Candidate Genes and Identifying Risk Factors for Leg Disease in Broilers: A Mendelian Randomization Study DOI Open Access

Xinxin Tang,

Peihao Liu, Na Luo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8890 - 8890

Published: Aug. 15, 2024

Clinical investigations have highlighted disruptions in bone metabolic processes and abnormal fluctuations serum indicator levels during the onset of leg disease (LD) broilers. However, presence a genetic causal relationship for this association remains undetermined. Therefore, aim study is to discern risk factors underlying LD development using 1235 sequenced white-feathered We employed Mendelian randomization (MR) analysis assess associations strength (BS), mineral density (BMD), tibial weight (TBW), length (TBL), diameter (TBD), ash (BA), calcium (Ash Ca), phosphorus P), (Ca), (P), alkaline phosphatase (ALP), osteoprotegerin (OPG) with incidence LD. Compelling evidence underscores link between developing decreased BMD (odds ratio (OR) = 0.998; 95% CI: 0.983, 0.993; P < 0.001) narrower TBD (OR 0.985, 0.975, 0.994, 0.002). Additionally, OPG concentrations (OR: 0.995, 0.992, 0.999, 0.008) were associated 0.0078, CI 0.0043 0.0140, 0.001), indicating robust ALP 0.988, 0.984, 0.993, 0.0046, 0.0026, 0.0083, 0.001). Moreover, elevated Ca 0.564, 0.487, 0.655, 0.614, 0.539, 0.699, TBD. Elevated Ca, P, ALP, contribute disturbances metabolism, while are greater This discovery elucidates broilers could provide information on LDs, such as osteoporosis, humans.

Language: Английский

Citations

0