Environmental DNA: Enhancing Species Detection and Study Comparability with Synthetic DNA Tracers DOI Open Access

George Stamatoyannopoulos,

Thalia Papayannopoulou

Опубликована: Фев. 2, 2024

Environmental DNA (eDNA) sampling has emerged as a powerful approach for monitoring biodiversity in the context of diverse terrestrial and aquatic ecosystems, broad potential both environmental surveys species management. eDNA studies focusing on plant animal have capitalized analytical methodologies developed originally metagenomic microorganisms, technological improvements sample collection. However, while proliferated rapidly, efforts to standardize methods are still nascent. Critically, fundamental data concerning distribution persistence different ecological contexts lacking, complicating confidence detection comparability between studies. We propose that key deficits could be addressed by using well-designed synthetic standards or tracers, which widely used fields ranging from medical diagnostics hydrology. In-field application standardized mixture barcoded fragments varying lengths concentrations recovered concommitantly with should provide critical yet missing turnover, well an internal molecular standard enhance

Язык: Английский

The Application of eDNA for Monitoring Aquatic Non-Indigenous Species: Practical and Policy Considerations DOI Creative Commons
Vera G. Fonseca, Phil I. Davison, Véronique Creach

и другие.

Diversity, Год журнала: 2023, Номер 15(5), С. 631 - 631

Опубликована: Май 6, 2023

Aquatic non-indigenous species (NIS) threaten biodiversity, ecosystem functions, and the economy worldwide. Monitoring NIS is of immediate concern to identify newly arriving species, assess efficacy mitigation measures, report long-term indicators introduction, spread, impacts. The challenges associated with conventional methods specimen collection morphological identification have led development alternative methods, such as DNA-based which could offer rapid cost-effective detection NIS. Depending on whether a few (targeted monitoring) or many (passive are being monitored, environmental DNA (eDNA) can infer presence-absence relative abundances, enabling informed decisions actions be made based patterns detection. Compared more eDNA tools increase levels sensitivity for rare elusive even noticeable some taxa when using targeted monitoring. use not only minimizes onus taxonomic expertise reduces resource demands but also sensitive cost-efficient in detecting NIS, thus proving its value an early warning tool. As nucleic acid (DNA/RNA) advance rapidly detection, there must balance between method sensitivity, logistical requirements, costs, factored into future management decisions. While complementary reviews available, our aim emphasize importance incorporating surveys highlight available opportunities this field.

Язык: Английский

Процитировано

29
How does parasite environmental transmission stage concentration change before, during, and after disease outbreaks? DOI Creative Commons
Elizabeth S. Davenport, Marcin K. Dziuba,

L. Jacobson

и другие.

Ecology, Год журнала: 2024, Номер 105(2)

Опубликована: Янв. 7, 2024

Abstract Outbreaks of environmentally transmitted parasites require that susceptible hosts encounter transmission stages in the environment and become infected, but we also know can be without triggering disease outbreaks. One challenge understanding relationship between environmental outbreaks is distribution abundance outside their have been difficult to quantify. Thus, limited data about how changes stage influence dynamics; moreover, do not whether differs among parasite species. We used digital PCR quantify five six lakes southeastern Michigan every 2 weeks from June November 2021. At same time, quantified infection prevalence host density. Our study focused on eight zooplankton species ( Daphnia spp. Ceriodaphnia dubia ) diverse taxonomic groups (bacteria, yeast, microsporidia, oomycete) with different mechanisms. found concentration increased prior for all parasites. However, differed significantly relative timing peaks The “continuous shedder” had at time as or slightly after outbreak peaks. In contrast, relying death (“obligate killers”) before For most parasites, higher spore concentrations than those outbreaks, especially once an began; exception was a parasite, Pasteuria ramosa , very strong genotypic specificity infection. Overall, our results show are tightly linked concentration; were preceded by increases then fueled production more during itself, decreasing pre‐outbreak levels waned. tracking improves drivers reveals traits may affect these dynamics.

Язык: Английский

Процитировано

11
Consumption of trematode parasite infectious stages: from conceptual synthesis to future research agenda DOI Creative Commons
Janet Koprivnikar, David W. Thieltges, Pieter T. J. Johnson

и другие.

Journal of Helminthology, Год журнала: 2023, Номер 97

Опубликована: Янв. 1, 2023

Abstract Given their sheer cumulative biomass and ubiquitous presence, parasites are increasingly recognized as essential components of most food webs. Beyond influence consumers host tissue, many also have free-living infectious stages that may be ingested by non-host organisms, with implications for energy nutrient transfer, well pathogen transmission disease dynamics. This has been particularly well-documented the cercaria stage digenean trematode within Phylum Platyhelminthes. Here, we aim to synthesize current state knowledge regarding cercariae consumption examining: (a) approaches studying consumption; (b) range prey documented thus far; (c) factors influencing likelihood (d) consequences individual predators (e.g. viability a source); (e) entire communities ecosystems transmission, cycling influences on other prey). We detected 121 unique consumer-by-cercaria combinations spanned 60 species consumer 35 species. Meaningful reductions in were seen 31 36 considered this; however, separate studies same sometimes showed different results. Along addressing gaps suggesting future research directions, highlight how conceptual empirical discussed here relevant pathogens, illustrating use model system help advance our general importance parasite consumption.

Язык: Английский

Процитировано

21
Building a pathway to One Health surveillance and response in Asian countries DOI Creative Commons
ROGER S. MORRIS, Shiyong Wang

Science in One Health, Год журнала: 2024, Номер 3, С. 100067 - 100067

Опубликована: Янв. 1, 2024

To detect and respond to emerging diseases more effectively, an integrated surveillance strategy needs be applied both human animal health. Current programs in Asian countries operate separately for the two sectors are principally concerned with detection of events that represent a short-term disease threat. It is not realistic either invest only efforts diseases, or rely solely on event-based surveillance. A comprehensive needed, concurrently investigating managing endemic zoonoses, studying evolving which change their character importance due influences such as demographic climatic change, enhancing understanding factors likely influence emergence new pathogens. This requires utilisation additional investigation tools have become available recent years but yet being used full effect. As there no fully formed blueprint can countries. Hence three-step pathway proposed move towards goal One Health response.

Язык: Английский

Процитировано

7
A One Health framework for exploring zoonotic interactions demonstrated through a case study DOI Creative Commons
Amélie Desvars-Larrive, A Vogl, Gavrila Amadea Puspitarani

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 15, 2024

Abstract The eco-epidemiology of zoonoses is often oversimplified to host-pathogen interactions while findings derived from global datasets are rarely directly transferable smaller-scale contexts. Through a systematic literature search, we compiled dataset naturally occurring zoonotic in Austria, spanning 1975–2022. We introduce the concept web describe complex relationships between agents, their hosts, vectors, food, and environmental sources. was explored through network analysis. After controlling for research effort, demonstrate that, within projected unipartite source-source agent sharing, most influential sources human, cattle, chicken, some meat products. Analysis One Health 3-cliques (triangular sets nodes representing animal, environment) confirms increased probability spillover at human-cattle human-food interfaces. characterise six communities which assembly patterns likely driven by highly connected infectious agents web, proximity anthropogenic activities. Additionally, report frequency emerging diseases Austria one every years. Here, present flexible network-based approach that offers insights into transmission chains, facilitating development locally-relevant strategies against zoonoses.

Язык: Английский

Процитировано

6
Gaps in parasitological research in the molecular era DOI
Tomáš Scholz

Trends in Parasitology, Год журнала: 2024, Номер 40(4), С. 283 - 291

Опубликована: Фев. 29, 2024

Язык: Английский

Процитировано

5
Development of a qPCR Duplex Assay for simultaneous detection of Fascioloides magna and Galba truncatula in eDNA samples: Monitoring beyond boundaries DOI Creative Commons
Amir Reza Varzandi, Stefania Zanet,

Elisa Rubele

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 916, С. 170338 - 170338

Опубликована: Янв. 23, 2024

Parasites constitute a significant economic burden and highly impact environmental, public, animal health. The emergence of many parasitic diseases is environmentally mediated they share the same biogeography with humans both domestic wild animals. American liver fluke, Fascioloides magna – trematode parasite ungulates an example anthropogenic introduction “invasive alien species” in Italy Europe. Multiple introductions to Europe have led biogeographical expansion across Danube region mainly provided by presence suitable habitats for all hosts involved parasite's life cycle, human-assisted transport, drastic environmental events such as flooding. In Italy, it was introduced established La Mandria Regional Park (LMRP) near Turin 1865 along imported wapitis (Cervus elaphus canadensis) from North America (Bassi, 1875), but no reported surrounding areas. LMRP isolated F. focus, poses important threat possible since enclosed area vulnerable occasional incursions roe deer. Additionally, tributary rivers Po river system, traversing area, could further bolster possibility spread. this study, we developed duplex qPCR assay its principal intermediate host Galba truncatula optimized testing eDNA samples meet needs surveillance parasite. Moreover, validated natura derived filtered water sediments inside outside LMRP's fenced-off area. Our findings first time demonstrate magna's park's internal

Язык: Английский

Процитировано

4
Nanoplasmonic biosensors for environmental sustainability and human health DOI
Wenpeng Liu, Kyungwha Chung, Subin Yu

и другие.

Chemical Society Reviews, Год журнала: 2024, Номер 53(21), С. 10491 - 10522

Опубликована: Янв. 1, 2024

Monitoring the health conditions of environment and humans is essential for ensuring human well-being, promoting global health, achieving sustainability. Innovative biosensors are crucial in accurately monitoring conditions, uncovering hidden connections between understanding how environmental factors trigger autoimmune diseases, neurodegenerative infectious diseases. This review evaluates use nanoplasmonic that can monitor diseases according to target analytes different sizes scales, providing valuable insights preventive medicine. We begin by explaining fundamental principles mechanisms biosensors. investigate potential techniques detecting various biological molecules, extracellular vesicles (EVs), pathogens, cells. also explore possibility wearable physiological network healthy connectivity humans, animals, plants, organisms. will guide design next-generation advance sustainable healthcare environment, planet.

Язык: Английский

Процитировано

4
Preserving Darwin’s fox: genomic tools for the conservation of South America’s most endangered canid DOI Creative Commons

Cristóbal Valenzuela-Turner,

José Horacio Grau,

Jörns Fickel

и другие.

Frontiers in Conservation Science, Год журнала: 2025, Номер 6

Опубликована: Янв. 23, 2025

Advances in high-throughput sequencing (HTS) have made it a powerful resource for the conservation of threatened species, providing information at both population and individual levels to inform management decisions. In South America, however, application HTS has been limited, primarily due challenges funding access advanced genomic equipment analytical expertise. Darwin’s fox ( Lycalopex fulvipes ), endemic Chile’s Valdivian Temperate Rainforest, is most endangered canid America with small declining estimated less than 1000 mature individuals. Despite its status, significant knowledge gaps remain. Here we highlight potential address these challenges, such as clarifying taxonomy, demographic history, geographic distribution, structure, genetic diversity, pathogen exposure. Integrating molecular data into planning will be pivotal ensuring long-term survival by identifying priorities targeted interventions, highlighting areas critical habitat conservation, guiding rescue efforts enhance diversity resilience.

Язык: Английский

Процитировано

0
Unveiling the Threat of Disease X: Preparing for the Next Global Pandemic DOI Open Access
Mengyuan Zhao,

Luping Lei,

Yinghan Jiang

и другие.

Journal of Medical Virology, Год журнала: 2025, Номер 97(2)

Опубликована: Фев. 1, 2025

ABSTRACT The term “Disease X”, first introduced by the World Health Organization (WHO) in 2018, symbolizes threat of an unknown pathogen capable causing a global pandemic. Classified as “priority pathogens,” Disease X stands alongside well‐known threats like SARS, Ebola, and ZIKV due to its potential for widespread outbreaks. SARS‐CoV‐2 is considered X” fulfill this prediction, demonstrating devastating impact such pathogens can have. A future could pose even greater threat, with catastrophic consequences. This paper examines origins pathogens, drawing lessons from outbreaks MERS, SARS‐CoV‐2. It also highlights strategic approaches detect, prevent, respond effectively mitigate risk pandemics.

Язык: Английский

Процитировано

0